Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5JET4 (RADA_THEKO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA repair and recombination protein RadA

Cleaved into the following chain:

  1. Pko RadA intein
Gene names
Name:radA
Ordered Locus Names:TK1899
OrganismThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]
Taxonomic identifier69014 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length836 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules By similarity. HAMAP-Rule MF_00348

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00348

Miscellaneous

The intein interrupts the ATP-binding site.

Sequence similarities

Belongs to the eukaryotic RecA-like protein family.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 149149DNA repair and recombination protein RadA, 1st part Potential
PRO_0000030164
Chain150 – 631482Pko RadA intein Potential
PRO_0000030165
Chain632 – 836205DNA repair and recombination protein RadA, 2nd part Potential
PRO_0000030166

Regions

Domain285 – 410126DOD-type homing endonuclease

Sequences

Sequence LengthMass (Da)Tools
Q5JET4 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 57387B98C8317F04

FASTA83693,707
        10         20         30         40         50         60 
MARKKKVEDE VKELEEFEEL DVEESLSSSD KQSKPEKKIS ALEDLPGVGP ATAEKLREAG 

        70         80         90        100        110        120 
YDTIEAIAVA SPLELKEIAG ISEGAALKII QAAREAANIG TFMRADEYMK RRTTIGKIST 

       130        140        150        160        170        180 
GSKALDKLLG GGIETQAITE VFGEFGSGKC FAKDTKVYYE NDTLVHFESI EDMYHKYASL 

       190        200        210        220        230        240 
GREVPFDNGY AVPLETVSVY TFDPKTGEVK RTKASYIYRE KVEKLAEIRL SNGYLLRITL 

       250        260        270        280        290        300 
LHPVLVFRNG LQWVPAGMIK PGDLIVGIRS VPANAATIEE SEAYFLGLFV AEGTSNPLSI 

       310        320        330        340        350        360 
TTGSEELKDF IVSFIEDHDG YTPTVEVRRG LYRILFRKKT AEWLGELATS NASTKVVPER 

       370        380        390        400        410        420 
VLNAGESAIA AFLAGYLDGD GYLTESIVEL VTKSRELADG LVFLLKRLGI TPRISQKTIE 

       430        440        450        460        470        480 
GSVYYRIYIT GEDRKTFEKV LEKSRIKPGE MNEGGVGRYP PALGKFLGKL YSEFRLPKRD 

       490        500        510        520        530        540 
NETAYHILTR SRNVWFTEKT LSRIEEYFRE ALEKLSEARK ALEMGDKPEL PFPWTAITKY 

       550        560        570        580        590        600 
GFTDRQVANY RTRGLPKRPE LKEKVVSALL KEIERLEGVA KLALETIELA RRLEFHEVSS 

       610        620        630        640        650        660 
VEVVDYNDWV YDLVIPETHN FIAPNGLVLH NTQLAHTLAV MVQKPPEEGG LGGSVIWIDT 

       670        680        690        700        710        720 
ENTFRPERIK QIAENRGLDP EETLKNIYVA RAFNSNHQML LVEKAEEIIK EKAESDRPVK 

       730        740        750        760        770        780 
LLVVDSLMAH FRAEYVGRGT LAERQQKLAK HLADLHRLAD LYDIAVFVTN QVQAKPDAFF 

       790        800        810        820        830 
GDPTRPVGGH ILAHSATLRV YLRKGKAGKR VARLIDSPHL PEGEAVFRIT EKGVED 

« Hide

References

[1]"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006878 Genomic DNA. Translation: BAD86088.1.
RefSeqYP_184312.1. NC_006624.1.

3D structure databases

ProteinModelPortalQ5JET4.
SMRQ5JET4. Positions 41-149, 632-836.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ5JET4. 1 interaction.
MINTMINT-8377210.
STRING69014.TK1899.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD86088; BAD86088; TK1899.
GeneID3233960.
KEGGtko:TK1899.

Phylogenomic databases

eggNOGCOG1372.
HOGENOMHOG000154068.
KOK04483.
OMASGKCFAK.

Enzyme and pathway databases

BioCycTKOD69014:GH72-1936-MONOMER.

Family and domain databases

Gene3D2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPMF_00348. RadA.
InterProIPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamPF14528. LAGLIDADG_3. 1 hit.
PF08423. Rad51. 2 hits.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF47794. SSF47794. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02236. recomb_radA. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS50162. RECA_2. 2 hits.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRADA_THEKO
AccessionPrimary (citable) accession number: Q5JET4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries