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Q5JET4

- RADA_THEKO

UniProt

Q5JET4 - RADA_THEKO

Protein

DNA repair and recombination protein RadA

Gene

radA

Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. damaged DNA binding Source: InterPro
    3. DNA-dependent ATPase activity Source: InterPro
    4. endonuclease activity Source: UniProtKB-KW

    GO - Biological processi

    1. DNA repair Source: InterPro
    2. intein-mediated protein splicing Source: InterPro
    3. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, Intron homing

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciTKOD69014:GH72-1936-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair and recombination protein RadA
    Cleaved into the following chain:
    Gene namesi
    Name:radA
    Ordered Locus Names:TK1899
    OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
    Taxonomic identifieri69014 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    ProteomesiUP000000536: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 149149DNA repair and recombination protein RadA, 1st partSequence AnalysisPRO_0000030164Add
    BLAST
    Chaini150 – 631482Pko RadA inteinSequence AnalysisPRO_0000030165Add
    BLAST
    Chaini632 – 836205DNA repair and recombination protein RadA, 2nd partSequence AnalysisPRO_0000030166Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Protein-protein interaction databases

    IntActiQ5JET4. 1 interaction.
    MINTiMINT-8377210.
    STRINGi69014.TK1899.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5JET4.
    SMRiQ5JET4. Positions 41-149, 632-836.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini285 – 410126DOD-type homing endonucleaseAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1372.
    HOGENOMiHOG000154068.
    KOiK04483.
    OMAiSGKCFAK.

    Family and domain databases

    Gene3Di2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPiMF_00348. RadA.
    InterProiIPR013632. DNA_recomb/repair_Rad51_C.
    IPR011938. DNA_recomb/repair_RadA.
    IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    IPR020587. RecA_monomer-monomer_interface.
    [Graphical view]
    PfamiPF14528. LAGLIDADG_3. 1 hit.
    PF08423. Rad51. 2 hits.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00278. HhH1. 2 hits.
    SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF47794. SSF47794. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 2 hits.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR02236. recomb_radA. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS50162. RECA_2. 2 hits.
    PS50163. RECA_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5JET4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARKKKVEDE VKELEEFEEL DVEESLSSSD KQSKPEKKIS ALEDLPGVGP    50
    ATAEKLREAG YDTIEAIAVA SPLELKEIAG ISEGAALKII QAAREAANIG 100
    TFMRADEYMK RRTTIGKIST GSKALDKLLG GGIETQAITE VFGEFGSGKC 150
    FAKDTKVYYE NDTLVHFESI EDMYHKYASL GREVPFDNGY AVPLETVSVY 200
    TFDPKTGEVK RTKASYIYRE KVEKLAEIRL SNGYLLRITL LHPVLVFRNG 250
    LQWVPAGMIK PGDLIVGIRS VPANAATIEE SEAYFLGLFV AEGTSNPLSI 300
    TTGSEELKDF IVSFIEDHDG YTPTVEVRRG LYRILFRKKT AEWLGELATS 350
    NASTKVVPER VLNAGESAIA AFLAGYLDGD GYLTESIVEL VTKSRELADG 400
    LVFLLKRLGI TPRISQKTIE GSVYYRIYIT GEDRKTFEKV LEKSRIKPGE 450
    MNEGGVGRYP PALGKFLGKL YSEFRLPKRD NETAYHILTR SRNVWFTEKT 500
    LSRIEEYFRE ALEKLSEARK ALEMGDKPEL PFPWTAITKY GFTDRQVANY 550
    RTRGLPKRPE LKEKVVSALL KEIERLEGVA KLALETIELA RRLEFHEVSS 600
    VEVVDYNDWV YDLVIPETHN FIAPNGLVLH NTQLAHTLAV MVQKPPEEGG 650
    LGGSVIWIDT ENTFRPERIK QIAENRGLDP EETLKNIYVA RAFNSNHQML 700
    LVEKAEEIIK EKAESDRPVK LLVVDSLMAH FRAEYVGRGT LAERQQKLAK 750
    HLADLHRLAD LYDIAVFVTN QVQAKPDAFF GDPTRPVGGH ILAHSATLRV 800
    YLRKGKAGKR VARLIDSPHL PEGEAVFRIT EKGVED 836
    Length:836
    Mass (Da):93,707
    Last modified:February 15, 2005 - v1
    Checksum:i57387B98C8317F04
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006878 Genomic DNA. Translation: BAD86088.1.
    RefSeqiYP_184312.1. NC_006624.1.

    Genome annotation databases

    EnsemblBacteriaiBAD86088; BAD86088; TK1899.
    GeneIDi3233960.
    KEGGitko:TK1899.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006878 Genomic DNA. Translation: BAD86088.1 .
    RefSeqi YP_184312.1. NC_006624.1.

    3D structure databases

    ProteinModelPortali Q5JET4.
    SMRi Q5JET4. Positions 41-149, 632-836.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q5JET4. 1 interaction.
    MINTi MINT-8377210.
    STRINGi 69014.TK1899.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD86088 ; BAD86088 ; TK1899 .
    GeneIDi 3233960.
    KEGGi tko:TK1899.

    Phylogenomic databases

    eggNOGi COG1372.
    HOGENOMi HOG000154068.
    KOi K04483.
    OMAi SGKCFAK.

    Enzyme and pathway databases

    BioCyci TKOD69014:GH72-1936-MONOMER.

    Family and domain databases

    Gene3Di 2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPi MF_00348. RadA.
    InterProi IPR013632. DNA_recomb/repair_Rad51_C.
    IPR011938. DNA_recomb/repair_RadA.
    IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    IPR020587. RecA_monomer-monomer_interface.
    [Graphical view ]
    Pfami PF14528. LAGLIDADG_3. 1 hit.
    PF08423. Rad51. 2 hits.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00278. HhH1. 2 hits.
    SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47794. SSF47794. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 2 hits.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR02236. recomb_radA. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS50162. RECA_2. 2 hits.
    PS50163. RECA_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
      Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
      Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-918 / JCM 12380 / KOD1.

    Entry informationi

    Entry nameiRADA_THEKO
    AccessioniPrimary (citable) accession number: Q5JET4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The intein interrupts the ATP-binding site.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3