Q5JET4 (RADA_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA repair and recombination protein RadA

Cleaved into the following chain:

1. Pko RadA intein

Gene names

Name:	radA
Ordered Locus Names:	TK1899

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus › Thermococcus kodakarensis

Protein attributes

Sequence length	836 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules By similarity. HAMAP-Rule MF_00348
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00348
Miscellaneous	The intein interrupts the ATP-binding site. HAMAP-Rule MF_00348
Sequence similarities	Belongs to the eukaryotic RecA-like protein family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	DNA damage DNA recombination Intron homing
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-dependent ATPase activity Inferred from electronic annotation. Source: InterPro damaged DNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	DNA repair and recombination protein RadA, 1st part Potential		PRO_0000030164
	Chain	150 – 631	482	Pko RadA intein Potential		PRO_0000030165
	Chain	632 – 836	205	DNA repair and recombination protein RadA, 2nd part Potential		PRO_0000030166
Regions
	Domain	285 – 410	126	DOD-type homing endonuclease

Sequences

	Sequence		Length	Mass (Da)
	Q5JET4 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 57387B98C8317F04	FASTA	836	93,707
	10 20 30 40 50 60 MARKKKVEDE VKELEEFEEL DVEESLSSSD KQSKPEKKIS ALEDLPGVGP ATAEKLREAG 70 80 90 100 110 120 YDTIEAIAVA SPLELKEIAG ISEGAALKII QAAREAANIG TFMRADEYMK RRTTIGKIST 130 140 150 160 170 180 GSKALDKLLG GGIETQAITE VFGEFGSGKC FAKDTKVYYE NDTLVHFESI EDMYHKYASL 190 200 210 220 230 240 GREVPFDNGY AVPLETVSVY TFDPKTGEVK RTKASYIYRE KVEKLAEIRL SNGYLLRITL 250 260 270 280 290 300 LHPVLVFRNG LQWVPAGMIK PGDLIVGIRS VPANAATIEE SEAYFLGLFV AEGTSNPLSI 310 320 330 340 350 360 TTGSEELKDF IVSFIEDHDG YTPTVEVRRG LYRILFRKKT AEWLGELATS NASTKVVPER 370 380 390 400 410 420 VLNAGESAIA AFLAGYLDGD GYLTESIVEL VTKSRELADG LVFLLKRLGI TPRISQKTIE 430 440 450 460 470 480 GSVYYRIYIT GEDRKTFEKV LEKSRIKPGE MNEGGVGRYP PALGKFLGKL YSEFRLPKRD 490 500 510 520 530 540 NETAYHILTR SRNVWFTEKT LSRIEEYFRE ALEKLSEARK ALEMGDKPEL PFPWTAITKY 550 560 570 580 590 600 GFTDRQVANY RTRGLPKRPE LKEKVVSALL KEIERLEGVA KLALETIELA RRLEFHEVSS 610 620 630 640 650 660 VEVVDYNDWV YDLVIPETHN FIAPNGLVLH NTQLAHTLAV MVQKPPEEGG LGGSVIWIDT 670 680 690 700 710 720 ENTFRPERIK QIAENRGLDP EETLKNIYVA RAFNSNHQML LVEKAEEIIK EKAESDRPVK 730 740 750 760 770 780 LLVVDSLMAH FRAEYVGRGT LAERQQKLAK HLADLHRLAD LYDIAVFVTN QVQAKPDAFF 790 800 810 820 830 GDPTRPVGGH ILAHSATLRV YLRKGKAGKR VARLIDSPHL PEGEAVFRIT EKGVED

References

[1]

"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006878 Genomic DNA. Translation: BAD86088.1.
RefSeq	YP_184312.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q5JET4.
SMR	Q5JET4. Positions 41-149, 632-836.
ModBase	Search...
Protein-protein interaction databases
STRING	69014.TK1899.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD86088; BAD86088; TK1899.
GeneID	3233960.
KEGG	tko:TK1899.
Phylogenomic databases
eggNOG	COG1372.
HOGENOM	HOG000154068.
OMA	HNTQLAH.
ProtClustDB	CLSK2767754.
Family and domain databases
HAMAP	MF_00348. RadA.
InterPro	IPR013632. DNA_recomb/repair_Rad51_C. IPR011938. DNA_recomb/repair_RadA. IPR020588. DNA_recomb_RecA/RadB_ATP-bd. IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR020587. RecA_monomer-monomer_interface. [Graphical view]
PANTHER	PTHR22942:SF11. PTHR22942:SF11. 1 hit.
Pfam	PF08423. Rad51. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00278. HhH1. 2 hits. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF47794. Rad51_N. 1 hit.
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR02236. recomb_radA. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS50162. RECA_2. 2 hits. PS50163. RECA_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RADA_PYRKO

Accession

Primary (citable) accession number: Q5JET4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program