SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5JET4

- RADA_THEKO

UniProt

Q5JET4 - RADA_THEKO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA repair and recombination protein RadA

Gene
radA, TK1899
Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules By similarity.UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. damaged DNA binding Source: InterPro
  3. DNA-dependent ATPase activity Source: InterPro
  4. endonuclease activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA repair Source: InterPro
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, Intron homing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTKOD69014:GH72-1936-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair and recombination protein RadA
Cleaved into the following chain:
Gene namesi
Name:radA
Ordered Locus Names:TK1899
OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000000536: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 149149DNA repair and recombination protein RadA, 1st part Reviewed predictionPRO_0000030164Add
BLAST
Chaini150 – 631482Pko RadA intein Reviewed predictionPRO_0000030165Add
BLAST
Chaini632 – 836205DNA repair and recombination protein RadA, 2nd part Reviewed predictionPRO_0000030166Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Reviewed prediction.UniRule annotation

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

IntActiQ5JET4. 1 interaction.
MINTiMINT-8377210.
STRINGi69014.TK1899.

Structurei

3D structure databases

ProteinModelPortaliQ5JET4.
SMRiQ5JET4. Positions 41-149, 632-836.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini285 – 410126DOD-type homing endonucleaseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000154068.
KOiK04483.
OMAiSGKCFAK.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00348. RadA.
InterProiIPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF08423. Rad51. 2 hits.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02236. recomb_radA. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS50162. RECA_2. 2 hits.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5JET4-1 [UniParc]FASTAAdd to Basket

« Hide

MARKKKVEDE VKELEEFEEL DVEESLSSSD KQSKPEKKIS ALEDLPGVGP    50
ATAEKLREAG YDTIEAIAVA SPLELKEIAG ISEGAALKII QAAREAANIG 100
TFMRADEYMK RRTTIGKIST GSKALDKLLG GGIETQAITE VFGEFGSGKC 150
FAKDTKVYYE NDTLVHFESI EDMYHKYASL GREVPFDNGY AVPLETVSVY 200
TFDPKTGEVK RTKASYIYRE KVEKLAEIRL SNGYLLRITL LHPVLVFRNG 250
LQWVPAGMIK PGDLIVGIRS VPANAATIEE SEAYFLGLFV AEGTSNPLSI 300
TTGSEELKDF IVSFIEDHDG YTPTVEVRRG LYRILFRKKT AEWLGELATS 350
NASTKVVPER VLNAGESAIA AFLAGYLDGD GYLTESIVEL VTKSRELADG 400
LVFLLKRLGI TPRISQKTIE GSVYYRIYIT GEDRKTFEKV LEKSRIKPGE 450
MNEGGVGRYP PALGKFLGKL YSEFRLPKRD NETAYHILTR SRNVWFTEKT 500
LSRIEEYFRE ALEKLSEARK ALEMGDKPEL PFPWTAITKY GFTDRQVANY 550
RTRGLPKRPE LKEKVVSALL KEIERLEGVA KLALETIELA RRLEFHEVSS 600
VEVVDYNDWV YDLVIPETHN FIAPNGLVLH NTQLAHTLAV MVQKPPEEGG 650
LGGSVIWIDT ENTFRPERIK QIAENRGLDP EETLKNIYVA RAFNSNHQML 700
LVEKAEEIIK EKAESDRPVK LLVVDSLMAH FRAEYVGRGT LAERQQKLAK 750
HLADLHRLAD LYDIAVFVTN QVQAKPDAFF GDPTRPVGGH ILAHSATLRV 800
YLRKGKAGKR VARLIDSPHL PEGEAVFRIT EKGVED 836
Length:836
Mass (Da):93,707
Last modified:February 15, 2005 - v1
Checksum:i57387B98C8317F04
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006878 Genomic DNA. Translation: BAD86088.1.
RefSeqiYP_184312.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD86088; BAD86088; TK1899.
GeneIDi3233960.
KEGGitko:TK1899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006878 Genomic DNA. Translation: BAD86088.1 .
RefSeqi YP_184312.1. NC_006624.1.

3D structure databases

ProteinModelPortali Q5JET4.
SMRi Q5JET4. Positions 41-149, 632-836.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q5JET4. 1 interaction.
MINTi MINT-8377210.
STRINGi 69014.TK1899.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD86088 ; BAD86088 ; TK1899 .
GeneIDi 3233960.
KEGGi tko:TK1899.

Phylogenomic databases

eggNOGi COG1372.
HOGENOMi HOG000154068.
KOi K04483.
OMAi SGKCFAK.

Enzyme and pathway databases

BioCyci TKOD69014:GH72-1936-MONOMER.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPi MF_00348. RadA.
InterProi IPR013632. DNA_recomb/repair_Rad51_C.
IPR011938. DNA_recomb/repair_RadA.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view ]
Pfami PF14528. LAGLIDADG_3. 1 hit.
PF08423. Rad51. 2 hits.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00278. HhH1. 2 hits.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF47794. SSF47794. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02236. recomb_radA. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS50162. RECA_2. 2 hits.
PS50163. RECA_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
    Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
    Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Entry informationi

Entry nameiRADA_THEKO
AccessioniPrimary (citable) accession number: Q5JET4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the ATP-binding site.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi