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Protein

Ribonuclease Z

Gene

rnz

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.

Catalytic activityi

Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61Zinc 1; catalytic1
Metal bindingi63Zinc 1; catalytic1
Active sitei65Proton acceptor1
Metal bindingi65Zinc 2; catalytic1
Metal bindingi66Zinc 2; catalytic1
Metal bindingi137Zinc 1; catalytic1
Metal bindingi207Zinc 1; catalytic1
Metal bindingi207Zinc 2; catalytic1
Metal bindingi263Zinc 2; catalytic1

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processtRNA processing
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease Z (EC:3.1.26.11)
Short name:
RNase Z
Alternative name(s):
tRNA 3 endonuclease
tRNase Z
Gene namesi
Name:rnz
Ordered Locus Names:TK1114
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001559351 – 314Ribonuclease ZAdd BLAST314

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiQ5JE70. 1 interactor.
MINTiMINT-8376877.
STRINGi69014.TK1114.

Structurei

3D structure databases

ProteinModelPortaliQ5JE70.
SMRiQ5JE70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase Z family.

Phylogenomic databases

eggNOGiarCOG00501. Archaea.
COG1234. LUCA.
HOGENOMiHOG000272419.
InParanoidiQ5JE70.
KOiK00784.
OMAiGTQRQMM.
OrthoDBiPOG093Z04VY.

Family and domain databases

CDDicd07717. RNaseZ_ZiPD-like_MBL-fold. 1 hit.
Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN. 1 hit.
InterProiView protein in InterPro
IPR001279. Metallo-B-lactamas.
IPR036866. Metallo-hydrolase/OxRdtase.
IPR013471. RNase_Z/BN.
PfamiView protein in Pfam
PF12706. Lactamase_B_2. 2 hits.
SMARTiView protein in SMART
SM00849. Lactamase_B. 1 hit.
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5JE70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEVFFLGTG GIMPTRERNV PAIALRYKGE IILFDAGEGT IRQMNTAKLS
60 70 80 90 100
PMKVDKIFIT HFHGDHYLGI PALIQTMNLW DRQKPLHIYG PKYTFQFVQN
110 120 130 140 150
LLNSGFFRPG FDIHVHELGE TRLKFGDYEI WSFKVEHGIP ALGYVFKEKD
160 170 180 190 200
RRGKFLKEKL REYGLEEGPI LGKLEREGKI EWNGRIIRLE DVTGPRRKGL
210 220 230 240 250
KIVYTGDTEP CERVKLFSER ADLLIHEATY LNPGDRGDSY HSTVEEACDT
260 270 280 290 300
ARRAKVKLLA LFHRAFRYSY EDYVEEALKI CESLGVRAVV PRDFDVITFK
310
SGTWELRNLL EERE
Length:314
Mass (Da):36,419
Last modified:February 15, 2005 - v1
Checksum:i87CB40F0F29E3684
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA. Translation: BAD85303.1.
RefSeqiWP_011250065.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD85303; BAD85303; TK1114.
GeneIDi3234746.
KEGGitko:TK1114.
PATRICifig|69014.16.peg.1090.

Similar proteinsi

Entry informationi

Entry nameiRNZ_THEKO
AccessioniPrimary (citable) accession number: Q5JE70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: October 25, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families