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Protein

1,4-alpha-glucan branching enzyme TK1436

Gene

TK1436

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of branch points in alpha-glucans by cleavage of an alpha-1,4 glycosidic bond and subsequent transfer of the cleaved-off oligosaccharide to a new alpha-1,6 position. The branch chain-length distribution of the reaction products shows degree of polymerization (DP) of 5 to 30, with two local maxima at DP 6 and DP 11. Exhibits an alpha-retaining catalytic mechanism. Does not display alpha-galactosidase or pullulanase activity, since melibiose and pullulan are not substrates. Is not able to catalyze the hydrolysis or transglycosylation of maltoheptaose, suggesting that the TK1436 protein contains neither alpha-amylase nor 4-alpha-glucanotransferase activity.1 Publication

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Temperature dependencei

Optimum temperature is 70 degrees Celsius. Is thermostable up to 90 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei183Nucleophile1 Publication1
Sitei233Transition state stabilizerCurated1
Binding sitei261Substrate1
Binding sitei278Substrate; via amide nitrogen1
Active sitei354Proton donor1 Publication1
Binding sitei407Substrate1
Binding sitei467Substrate1
Binding sitei476Substrate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciTKOD69014:GH72-1462-MONOMER.
BRENDAi2.4.1.18. 5246.

Protein family/group databases

CAZyiGH57. Glycoside Hydrolase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme TK1436 (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Branching enzyme
Short name:
BE
Gene namesi
Ordered Locus Names:TK1436
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004139731 – 6751,4-alpha-glucan branching enzyme TK1436Add BLAST675

Expressioni

Inductioni

Up-regulated by maltodextrin.1 Publication

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

STRINGi69014.TK1436.

Structurei

Secondary structure

1675
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 11Combined sources8
Beta strandi22 – 25Combined sources4
Helixi26 – 35Combined sources10
Helixi37 – 49Combined sources13
Beta strandi56 – 60Combined sources5
Helixi62 – 68Combined sources7
Helixi71 – 94Combined sources24
Helixi99 – 118Combined sources20
Turni119 – 121Combined sources3
Helixi123 – 132Combined sources10
Beta strandi135 – 141Combined sources7
Helixi148 – 150Combined sources3
Helixi154 – 172Combined sources19
Beta strandi177 – 180Combined sources4
Helixi182 – 184Combined sources3
Beta strandi189 – 193Combined sources5
Helixi195 – 197Combined sources3
Beta strandi199 – 202Combined sources4
Helixi205 – 210Combined sources6
Turni211 – 213Combined sources3
Beta strandi216 – 219Combined sources4
Helixi221 – 225Combined sources5
Beta strandi231 – 234Combined sources4
Beta strandi245 – 247Combined sources3
Beta strandi249 – 251Combined sources3
Beta strandi257 – 260Combined sources4
Helixi263 – 270Combined sources8
Turni272 – 274Combined sources3
Helixi276 – 278Combined sources3
Turni290 – 292Combined sources3
Beta strandi301 – 304Combined sources4
Helixi306 – 308Combined sources3
Helixi314 – 343Combined sources30
Beta strandi348 – 354Combined sources7
Helixi355 – 357Combined sources3
Turni359 – 361Combined sources3
Helixi365 – 378Combined sources14
Helixi386 – 391Combined sources6
Beta strandi398 – 400Combined sources3
Helixi409 – 411Combined sources3
Turni414 – 416Combined sources3
Turni419 – 423Combined sources5
Helixi424 – 442Combined sources19
Helixi447 – 463Combined sources17
Helixi467 – 473Combined sources7
Helixi478 – 505Combined sources28
Helixi510 – 519Combined sources10
Helixi529 – 531Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N8TX-ray2.40A1-562[»]
3N92X-ray2.89A1-562[»]
3N98X-ray1.87A1-562[»]
ProteinModelPortaliQ5JDJ7.
SMRiQ5JDJ7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5JDJ7.

Family & Domainsi

Domaini

The C-terminus contains two copies of a helix-hairpin-helix (HhH) motif that are dispensable for activity and thermal stability.1 Publication

Sequence similaritiesi

Belongs to the glycosyl hydrolase 57 family.Curated

Phylogenomic databases

eggNOGiarCOG03281. Archaea.
COG1543. LUCA.
HOGENOMiHOG000046905.
InParanoidiQ5JDJ7.
KOiK16149.
OMAiPECAYYE.

Family and domain databases

Gene3Di1.20.1430.10. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR015293. DUF1957.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR004300. Glyco_hydro_57_N.
[Graphical view]
PfamiPF09210. DUF1957. 1 hit.
PF03065. Glyco_hydro_57. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5JDJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGYLTFVLH THIPYVRKHG KWPFGEEWVF EAISETYIPL LMEFERLRDS
60 70 80 90 100
GVKFGIVINV TPVLAEQLTD EYMKKAFEEY MERKLKAMEE DLKSGKYDEK
110 120 130 140 150
AVSYMLNYFR KVYDYWKAIN GDIIGKLREL QDQGYVEVIT SAATHGYLPL
160 170 180 190 200
LGRDEAIRAQ IANGVATYEK HFGMKPKGIW LPECAYRPAG EWELPGGRKV
210 220 230 240 250
KRQGIEKFLE EFGLRYFFVE SRLIDEGPAS NVYGEVLIAD TEKTTLRPYW
260 270 280 290 300
IKGSNVAVFA RNRETGHQVW SAHYGYPGDF WYREFHKKAP KSGGQYWRIT
310 320 330 340 350
SKEVGLGEKE FYDPDKAMER VEEHARHFVS LVERLLREHE EKFGEKGIIV
360 370 380 390 400
APYDTELFGH WWFEGVKWLG RVLELLYQRG VETPTLSRFL EEYSGEKHEI
410 420 430 440 450
ELPEGSWGAN SDHSTWWNEE TEWTWPHIYR AEDRMVAIVS RFRGRDELTN
460 470 480 490 500
RVIEQLAREL LILEASDWQF LITTGQAKEY AKRRVLIHSR DFHRLANELV
510 520 530 540 550
RYVKIGEFDV KLLEELEERD NPFRPVVVGP YVSENPPELE EYVEPPEVPP
560 570 580 590 600
EKEETEEKPK VLTEKATSLA LAVKKVKPVK EETREVKKKA VEASKRGKRK
610 620 630 640 650
SSKSKRLPRK VSKKAPSKGP SDLLSIKGIG PKTFQKLKRA GVETIEDLKN
660 670
ANIEDLARKT GISTKRLKKF IAQVE
Length:675
Mass (Da):78,549
Last modified:February 15, 2005 - v1
Checksum:i857E34E04F23B27A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA. Translation: BAD85625.1.
RefSeqiWP_011250387.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD85625; BAD85625; TK1436.
GeneIDi3234278.
KEGGitko:TK1436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA. Translation: BAD85625.1.
RefSeqiWP_011250387.1. NC_006624.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N8TX-ray2.40A1-562[»]
3N92X-ray2.89A1-562[»]
3N98X-ray1.87A1-562[»]
ProteinModelPortaliQ5JDJ7.
SMRiQ5JDJ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi69014.TK1436.

Protein family/group databases

CAZyiGH57. Glycoside Hydrolase Family 57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD85625; BAD85625; TK1436.
GeneIDi3234278.
KEGGitko:TK1436.

Phylogenomic databases

eggNOGiarCOG03281. Archaea.
COG1543. LUCA.
HOGENOMiHOG000046905.
InParanoidiQ5JDJ7.
KOiK16149.
OMAiPECAYYE.

Enzyme and pathway databases

BioCyciTKOD69014:GH72-1462-MONOMER.
BRENDAi2.4.1.18. 5246.

Miscellaneous databases

EvolutionaryTraceiQ5JDJ7.

Family and domain databases

Gene3Di1.20.1430.10. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR015293. DUF1957.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR028995. Glyco_hydro_57/38_cen.
IPR004300. Glyco_hydro_57_N.
[Graphical view]
PfamiPF09210. DUF1957. 1 hit.
PF03065. Glyco_hydro_57. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBE_THEKO
AccessioniPrimary (citable) accession number: Q5JDJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.