Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ADP-dependent ribose-1-phosphate kinase

Gene

TK2029

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in nucleoside degradation. Phosphorylates ribose 1-phosphate (R1P) to ribose 1,5-bisphosphate. Can also act on deoxyribose 1-phosphate (dR1P), but is most active with R1P. ADP is the most preferred phosphate donor, followed by GDP and UDP.1 Publication

Catalytic activityi

ADP + alpha-D-ribose-1-phosphate = AMP + alpha-D-ribose 1,5-bisphosphate.1 Publication

Cofactori

Mg2+1 Publication, Ca2+1 PublicationNote: Can use Mg2+ and Ca2+ with equal efficiency in vitro, and to a lesser extent, Co2+.1 Publication

Kineticsi

kcat is 485 sec(-1) with ribose 1-phosphate as substrate. kcat is 73 sec(-1) with deoxyribose 1-phosphate as substrate. kcat is 612 sec(-1) with ADP as substrate. kcat is 189 sec(-1) with GDP as substrate.1 Publication
  1. KM=0.53 mM for ribose 1-phosphate (in the presence of Mg2+)1 Publication
  2. KM=12 mM for deoxyribose 1-phosphate (in the presence of Mg2+)1 Publication
  3. KM=7.3 mM for ADP (in the presence of Mg2+)1 Publication
  4. KM=19 mM for GDP (in the presence of Mg2+)1 Publication
  1. Vmax=912 µmol/min/mg enzyme with ribose 1-phosphate as substrate (in the presence of Mg2+)1 Publication
  2. Vmax=137 µmol/min/mg enzyme with deoxyribose 1-phosphate as substrate (in the presence of Mg2+)1 Publication
  3. Vmax=1150 µmol/min/mg enzyme toward ADP (in the presence of Mg2+)1 Publication
  4. Vmax=355 µmol/min/mg enzyme toward GDP (in the presence of Mg2+)1 Publication

pH dependencei

High levels of activity within a broad pH range of 5.0-8.0.1 Publication

Temperature dependencei

Optimum temperature is 85 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei232Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi200 – 205ADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Calcium, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-19660
TKOD69014:G1G2A-2043-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-dependent ribose-1-phosphate kinase1 Publication (EC:2.7.1.2121 Publication)
Short name:
ADP-R1P kinase1 Publication
Alternative name(s):
ADP:alpha-D-ribose-1-phosphate 5-phosphotransferaseCurated
Alpha-D-ribose-1-phosphate 5-kinase (ADP)Curated
Gene namesi
Ordered Locus Names:TK2029Imported
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004416691 – 294ADP-dependent ribose-1-phosphate kinaseAdd BLAST294

Interactioni

Protein-protein interaction databases

STRINGi69014.TK2029

Structurei

3D structure databases

ProteinModelPortaliQ5JDG9
SMRiQ5JDG9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family.Curated

Phylogenomic databases

eggNOGiarCOG00014 Archaea
COG0524 LUCA
HOGENOMiHOG000235950
InParanoidiQ5JDG9
KOiK21056
OMAiCALIVLQ
OrthoDBiPOG093Z05NI

Family and domain databases

Gene3Di3.40.1190.20, 1 hit
InterProiView protein in InterPro
IPR002173 Carboh/pur_kinase_PfkB_CS
IPR011611 PfkB_dom
IPR002139 Ribo/fructo_kinase
IPR029056 Ribokinase-like
PfamiView protein in Pfam
PF00294 PfkB, 1 hit
PRINTSiPR00990 RIBOKINASE
SUPFAMiSSF53613 SSF53613, 1 hit
PROSITEiView protein in PROSITE
PS00584 PFKB_KINASES_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q5JDG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLDVIGIGN LNYDIIFTLE RFPEFHEKIN ARGAHFGLGG AAANTISWLA
60 70 80 90 100
HFGLKTGYIG AVGNDDVGEM HIKYFQGIGV DTGGIDVVEE PSGVAVAMVA
110 120 130 140 150
GDDKRIVKYP GANLRRRFKP EYASRAKFLH LSSNPPELIE EAVNFASQRG
160 170 180 190 200
IKVSLDIGEA PLPRELESKV DYLMMNEDEY RRKYGSLDPS LCRAKNLVVT
210 220 230 240 250
LNGGGALVRE GDNVFEVRGL SAKVVDSTGA GDSFDAGVIY GVLNGWSLLD
260 270 280 290
SAKLGMLLAY LTVQKVGARS AIVPLEEVKR IAREVGLDLP FNRT
Length:294
Mass (Da):31,915
Last modified:February 15, 2005 - v1
Checksum:i49323269CB1C1BA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA Translation: BAD86218.1
RefSeqiWP_011250979.1, NC_006624.1

Genome annotation databases

EnsemblBacteriaiBAD86218; BAD86218; TK2029
GeneIDi3235391
KEGGitko:TK2029
PATRICifig|69014.16.peg.1983

Similar proteinsi

Entry informationi

Entry nameiR1PK_THEKO
AccessioniPrimary (citable) accession number: Q5JDG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2017
Last sequence update: February 15, 2005
Last modified: April 25, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health