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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Caution

Despite the fact that this protein belongs to a subfamily proposed to have ribose 1,5-bisphosphate isomerase activity (PubMed:15375115), the recombinant protein does not possess this activity (PubMed:17303759). In contrast, another protein from P.kodakaraensis has been shown to display this activity (TK0185).1 Publication

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADUniRule annotation1
Binding sitei61NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei125NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi153Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi168IronUniRule annotation1
Binding sitei216NAD; via amide nitrogenUniRule annotation1
Binding sitei226GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 54NADUniRule annotation2
Nucleotide bindingi151 – 153NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciTKOD69014:G1G2A-430-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:TK0434
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001539541 – 251Thiamine thiazole synthaseAdd BLAST251

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi69014.TK0434

Structurei

3D structure databases

ProteinModelPortaliQ5JD25
SMRiQ5JD25
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
InParanoidiQ5JD25
KOiK03146
OMAiMWGGGMM
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

Q5JD25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREIEISRAI VEAYFNDLLQ NLQLDIAIVG AGPSGMVAGY YLAKGGAKVA
60 70 80 90 100
IFEKKLSVGG GIWGGAMGFN RVVVQESARE ILDEFGVDYS QVGNGLYVLD
110 120 130 140 150
SIELASTLAS KAVKAGAKIF NMVEVEDLVV KDGRVSGLVI NWTPVMMTGL
160 170 180 190 200
HVDPLTVEAK FVVDSTGHGA QISQHLLKRG LIKAIPGEGP MWAEKGEELT
210 220 230 240 250
VEHTREVFPG LYATGMAANA LAGAPRMGPI FGGMLLSGRK AALEILQKLG

K
Length:251
Mass (Da):26,641
Last modified:February 15, 2005 - v1
Checksum:iE43CCF608C7CB1D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA Translation: BAD84623.1
RefSeqiWP_011249389.1, NC_006624.1

Genome annotation databases

EnsemblBacteriaiBAD84623; BAD84623; TK0434
GeneIDi3234250
KEGGitko:TK0434
PATRICifig|69014.16.peg.426

Similar proteinsi

Entry informationi

Entry nameiTHI4_THEKO
AccessioniPrimary (citable) accession number: Q5JD25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: March 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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