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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADUniRule annotation1
Binding sitei61NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei125NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi153Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi168IronUniRule annotation1
Binding sitei216NAD; via amide nitrogenUniRule annotation1
Binding sitei226GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 54NADUniRule annotation2
Nucleotide bindingi151 – 153NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciTKOD69014:GH72-443-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:TK0434
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001539541 – 251Thiamine thiazole synthaseAdd BLAST251

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi69014.TK0434.

Structurei

3D structure databases

ProteinModelPortaliQ5JD25.
SMRiQ5JD25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
InParanoidiQ5JD25.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiPOG093Z09PU.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5JD25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREIEISRAI VEAYFNDLLQ NLQLDIAIVG AGPSGMVAGY YLAKGGAKVA
60 70 80 90 100
IFEKKLSVGG GIWGGAMGFN RVVVQESARE ILDEFGVDYS QVGNGLYVLD
110 120 130 140 150
SIELASTLAS KAVKAGAKIF NMVEVEDLVV KDGRVSGLVI NWTPVMMTGL
160 170 180 190 200
HVDPLTVEAK FVVDSTGHGA QISQHLLKRG LIKAIPGEGP MWAEKGEELT
210 220 230 240 250
VEHTREVFPG LYATGMAANA LAGAPRMGPI FGGMLLSGRK AALEILQKLG

K
Length:251
Mass (Da):26,641
Last modified:February 15, 2005 - v1
Checksum:iE43CCF608C7CB1D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA. Translation: BAD84623.1.
RefSeqiWP_011249389.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD84623; BAD84623; TK0434.
GeneIDi3234250.
KEGGitko:TK0434.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006878 Genomic DNA. Translation: BAD84623.1.
RefSeqiWP_011249389.1. NC_006624.1.

3D structure databases

ProteinModelPortaliQ5JD25.
SMRiQ5JD25.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi69014.TK0434.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD84623; BAD84623; TK0434.
GeneIDi3234250.
KEGGitko:TK0434.

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
InParanoidiQ5JD25.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiPOG093Z09PU.

Enzyme and pathway databases

UniPathwayiUPA00060.
BioCyciTKOD69014:GH72-443-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_THEKO
AccessioniPrimary (citable) accession number: Q5JD25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: March 15, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Despite the fact that this protein belongs to a subfamily proposed to have ribose 1,5-bisphosphate isomerase activity (PubMed:15375115), the recombinant protein does not possess this activity (PubMed:17303759). In contrast, another protein from P.kodakaraensis has been shown to display this activity (TK0185).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.