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Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene

Gcnt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + O3-(beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein] = UDP + O3-(beta-D-galactosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->6))-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein].By similarity
UDP-N-acetyl-alpha-D-glucosamine + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-R = UDP + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-(beta-D-GlcNAc-(1->6))-beta-D-Gal-(1->4)-beta-D-GlcNAc-R.By similarity
UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-(N-acetyl-beta-D-glucosaminyl-(1->3))-N-acetyl-alpha-D-galactosaminyl-R.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • immunoglobulin production in mucosal tissue Source: MGI
  • intestinal absorption Source: MGI
  • kidney morphogenesis Source: MGI
  • protein O-linked glycosylation Source: MGI
  • tissue morphogenesis Source: MGI

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.102 3474
2.4.1.150 3474
ReactomeiR-MMU-913709 O-linked glycosylation of mucins
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT14 Glycosyltransferase Family 14

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.102By similarity, EC:2.4.1.148By similarity, EC:2.4.1.150By similarity)
Alternative name(s):
C2GnT-mucin type
Short name:
C2GnT-M
Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
Gene namesi
Name:Gcnt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1919327 Gcnt3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
Topological domaini31 – 437LumenalSequence analysisAdd BLAST407

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002885461 – 437Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3Add BLAST437

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 227By similarity
Disulfide bondi161 ↔ 381By similarity
Disulfide bondi182 ↔ 209By similarity
Glycosylationi288N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi390 ↔ 422By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ5JCT0
PaxDbiQ5JCT0
PeptideAtlasiQ5JCT0
PRIDEiQ5JCT0

PTM databases

PhosphoSitePlusiQ5JCT0

Expressioni

Gene expression databases

BgeeiENSMUSG00000032226
CleanExiMM_GCNT3
GenevisibleiQ5JCT0 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034751

Structurei

3D structure databases

ProteinModelPortaliQ5JCT0
SMRiQ5JCT0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0799 Eukaryota
ENOG410XQ7M LUCA
GeneTreeiENSGT00760000119183
HOGENOMiHOG000293251
HOVERGENiHBG051711
InParanoidiQ5JCT0
KOiK09662
OMAiHLLANKF
OrthoDBiEOG091G0HLZ
PhylomeDBiQ5JCT0
TreeFamiTF315534

Family and domain databases

InterProiView protein in InterPro
IPR003406 Glyco_trans_14
PfamiView protein in Pfam
PF02485 Branch, 1 hit

Sequencei

Sequence statusi: Complete.

Q5JCT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSWQRLCWH YRLWTLGCYM LLAILALKLS LRLKCDFDAM DLDSEEFQSQ
60 70 80 90 100
YCRDLLYKTL KLPAKSSINC SGVIRGEQKA VTQALLNNLE IKKKQQLFTE
110 120 130 140 150
ADYLRMTADC EHFKTKRKFI QVPLSKEEAS FPIAYSMVVH EKIENFERLL
160 170 180 190 200
RAVYTPQNVY CVHMDQKSSE PFKQAVRAIV SCFPNVFIAS KLVSVVYASW
210 220 230 240 250
SRVQADLNCM EDLLQSPVPW KYLLNTCGTD FPIKTNAEMV KALKLLKGQN
260 270 280 290 300
SMESEVPPPH KKSRWKYHYE VTDTLHMTSK RKTPPPNNLT MFTGNAYMVA
310 320 330 340 350
SRDFIEHVFS NSKARQLIEW VKDTYSPDEH LWATLQRASW MPGSDPLHRK
360 370 380 390 400
FDLSDMRAIA RLTKWYDHEG DIENGAPYTS CSGIHQRAVC VYGSGDLHWI
410 420 430
LQNHHLLANK FDPKVDDNVL QCLEEYLRHK AIYGTEL
Length:437
Mass (Da):50,698
Last modified:May 29, 2007 - v2
Checksum:iFF0C1EC8F25320F1
GO

Sequence cautioni

The sequence AAH18297 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB25548 differs from that shown. Reason: Erroneous termination at position 35. Translated as Cys.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101A → G in AAK72480 (Ref. 1) Curated1
Sequence conflicti353L → M in BAB25548 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY039027 mRNA Translation: AAK72480.1
EF202835 mRNA Translation: ABM91120.1
AK008234 mRNA Translation: BAB25548.1 Sequence problems.
BC018297 mRNA Translation: AAH18297.1 Different initiation.
CCDSiCCDS40678.1
RefSeqiNP_082363.2, NM_028087.2
XP_006511526.1, XM_006511463.3
XP_006511528.1, XM_006511465.3
XP_011241117.1, XM_011242815.2
XP_011241118.1, XM_011242816.2
XP_011241119.1, XM_011242817.2
UniGeneiMm.195555

Genome annotation databases

EnsembliENSMUST00000034751; ENSMUSP00000034751; ENSMUSG00000032226
GeneIDi72077
KEGGimmu:72077
UCSCiuc009qnt.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGCNT3_MOUSE
AccessioniPrimary (citable) accession number: Q5JCT0
Secondary accession number(s): A2TIK7, Q8VCX9, Q9D8A3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: April 25, 2018
This is version 98 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health