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Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene

Gcnt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathway:iprotein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • immunoglobulin production in mucosal tissue Source: MGI
  • intestinal absorption Source: MGI
  • kidney morphogenesis Source: MGI
  • protein O-linked glycosylation Source: MGI
  • tissue morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.102. 3474.
2.4.1.150. 3474.
ReactomeiREACT_278772. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.102, EC:2.4.1.150)
Alternative name(s):
C2GnT-mucin type
Short name:
C2GnT-M
Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
Gene namesi
Name:Gcnt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1919327. Gcnt3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 3018Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini31 – 437407LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3PRO_0000288546Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 227By similarity
Disulfide bondi161 ↔ 381By similarity
Disulfide bondi182 ↔ 209By similarity
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi390 ↔ 422By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ5JCT0.
PRIDEiQ5JCT0.

PTM databases

PhosphoSiteiQ5JCT0.

Expressioni

Gene expression databases

BgeeiQ5JCT0.
CleanExiMM_GCNT3.
GenevisibleiQ5JCT0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034751.

Structurei

3D structure databases

ProteinModelPortaliQ5JCT0.
SMRiQ5JCT0. Positions 67-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253565.
GeneTreeiENSGT00760000119183.
HOGENOMiHOG000293251.
HOVERGENiHBG051711.
InParanoidiQ5JCT0.
KOiK09662.
OMAiNITMFTG.
OrthoDBiEOG74N5HW.
PhylomeDBiQ5JCT0.
TreeFamiTF315534.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5JCT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSWQRLCWH YRLWTLGCYM LLAILALKLS LRLKCDFDAM DLDSEEFQSQ
60 70 80 90 100
YCRDLLYKTL KLPAKSSINC SGVIRGEQKA VTQALLNNLE IKKKQQLFTE
110 120 130 140 150
ADYLRMTADC EHFKTKRKFI QVPLSKEEAS FPIAYSMVVH EKIENFERLL
160 170 180 190 200
RAVYTPQNVY CVHMDQKSSE PFKQAVRAIV SCFPNVFIAS KLVSVVYASW
210 220 230 240 250
SRVQADLNCM EDLLQSPVPW KYLLNTCGTD FPIKTNAEMV KALKLLKGQN
260 270 280 290 300
SMESEVPPPH KKSRWKYHYE VTDTLHMTSK RKTPPPNNLT MFTGNAYMVA
310 320 330 340 350
SRDFIEHVFS NSKARQLIEW VKDTYSPDEH LWATLQRASW MPGSDPLHRK
360 370 380 390 400
FDLSDMRAIA RLTKWYDHEG DIENGAPYTS CSGIHQRAVC VYGSGDLHWI
410 420 430
LQNHHLLANK FDPKVDDNVL QCLEEYLRHK AIYGTEL
Length:437
Mass (Da):50,698
Last modified:May 29, 2007 - v2
Checksum:iFF0C1EC8F25320F1
GO

Sequence cautioni

The sequence AAH18297.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB25548.1 differs from that shown. Reason: Erroneous termination at position 35. Translated as Cys.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti101 – 1011A → G in AAK72480 (Ref. 1) Curated
Sequence conflicti353 – 3531L → M in BAB25548 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY039027 mRNA. Translation: AAK72480.1.
EF202835 mRNA. Translation: ABM91120.1.
AK008234 mRNA. Translation: BAB25548.1. Sequence problems.
BC018297 mRNA. Translation: AAH18297.1. Different initiation.
CCDSiCCDS40678.1.
RefSeqiNP_082363.2. NM_028087.2.
XP_006511526.1. XM_006511463.2.
XP_006511528.1. XM_006511465.2.
XP_011241117.1. XM_011242815.1.
XP_011241118.1. XM_011242816.1.
XP_011241119.1. XM_011242817.1.
UniGeneiMm.195555.

Genome annotation databases

EnsembliENSMUST00000034751; ENSMUSP00000034751; ENSMUSG00000032226.
GeneIDi72077.
KEGGimmu:72077.
UCSCiuc009qnt.1. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

core 2 beta 6 GlcNAc T3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY039027 mRNA. Translation: AAK72480.1.
EF202835 mRNA. Translation: ABM91120.1.
AK008234 mRNA. Translation: BAB25548.1. Sequence problems.
BC018297 mRNA. Translation: AAH18297.1. Different initiation.
CCDSiCCDS40678.1.
RefSeqiNP_082363.2. NM_028087.2.
XP_006511526.1. XM_006511463.2.
XP_006511528.1. XM_006511465.2.
XP_011241117.1. XM_011242815.1.
XP_011241118.1. XM_011242816.1.
XP_011241119.1. XM_011242817.1.
UniGeneiMm.195555.

3D structure databases

ProteinModelPortaliQ5JCT0.
SMRiQ5JCT0. Positions 67-432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034751.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

PTM databases

PhosphoSiteiQ5JCT0.

Proteomic databases

MaxQBiQ5JCT0.
PRIDEiQ5JCT0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034751; ENSMUSP00000034751; ENSMUSG00000032226.
GeneIDi72077.
KEGGimmu:72077.
UCSCiuc009qnt.1. mouse.

Organism-specific databases

CTDi9245.
MGIiMGI:1919327. Gcnt3.

Phylogenomic databases

eggNOGiNOG253565.
GeneTreeiENSGT00760000119183.
HOGENOMiHOG000293251.
HOVERGENiHBG051711.
InParanoidiQ5JCT0.
KOiK09662.
OMAiNITMFTG.
OrthoDBiEOG74N5HW.
PhylomeDBiQ5JCT0.
TreeFamiTF315534.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.102. 3474.
2.4.1.150. 3474.
ReactomeiREACT_278772. O-linked glycosylation of mucins.

Miscellaneous databases

ChiTaRSiGcnt3. mouse.
NextBioi335378.
PROiQ5JCT0.
SOURCEiSearch...

Gene expression databases

BgeeiQ5JCT0.
CleanExiMM_GCNT3.
GenevisibleiQ5JCT0. MM.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Core 2 beta-1,6-N-acetylglucosaminyltransferase II (Core2-GlcNAcT-II)."
    Yeh J.-C., Ong E., Fukuda M.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Mucin biosynthesis: molecular cloning and expression of mouse mucus-type core 2 beta-1,6 N-acetylglucosaminyltransferase."
    Hashimoto M., Tan S., Mori N., Cheng H., Cheng P.-W.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Colon.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Small intestine.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.

Entry informationi

Entry nameiGCNT3_MOUSE
AccessioniPrimary (citable) accession number: Q5JCT0
Secondary accession number(s): A2TIK7, Q8VCX9, Q9D8A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: July 22, 2015
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.