Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5JCT0

- GCNT3_MOUSE

UniProt

Q5JCT0 - GCNT3_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene

Gcnt3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathwayi

GO - Molecular functioni

  1. acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: MGI
  2. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  3. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. immunoglobulin production in mucosal tissue Source: MGI
  2. intestinal absorption Source: MGI
  3. kidney morphogenesis Source: MGI
  4. protein glycosylation Source: UniProtKB-UniPathway
  5. tissue morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_198517. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.102, EC:2.4.1.150)
Alternative name(s):
C2GnT-mucin type
Short name:
C2GnT-M
Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
Gene namesi
Name:Gcnt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1919327. Gcnt3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 3018Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini31 – 437407LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. Golgi apparatus Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3PRO_0000288546Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 227By similarity
Disulfide bondi161 ↔ 381By similarity
Disulfide bondi182 ↔ 209By similarity
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi390 ↔ 422By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ5JCT0.
PRIDEiQ5JCT0.

PTM databases

PhosphoSiteiQ5JCT0.

Expressioni

Gene expression databases

BgeeiQ5JCT0.
CleanExiMM_GCNT3.
GenevestigatoriQ5JCT0.

Structurei

3D structure databases

ProteinModelPortaliQ5JCT0.
SMRiQ5JCT0. Positions 67-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253565.
GeneTreeiENSGT00760000119183.
HOGENOMiHOG000293251.
HOVERGENiHBG051711.
InParanoidiQ5JCT0.
KOiK09662.
OMAiMTADCEH.
OrthoDBiEOG74N5HW.
PhylomeDBiQ5JCT0.
TreeFamiTF315534.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5JCT0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSWQRLCWH YRLWTLGCYM LLAILALKLS LRLKCDFDAM DLDSEEFQSQ
60 70 80 90 100
YCRDLLYKTL KLPAKSSINC SGVIRGEQKA VTQALLNNLE IKKKQQLFTE
110 120 130 140 150
ADYLRMTADC EHFKTKRKFI QVPLSKEEAS FPIAYSMVVH EKIENFERLL
160 170 180 190 200
RAVYTPQNVY CVHMDQKSSE PFKQAVRAIV SCFPNVFIAS KLVSVVYASW
210 220 230 240 250
SRVQADLNCM EDLLQSPVPW KYLLNTCGTD FPIKTNAEMV KALKLLKGQN
260 270 280 290 300
SMESEVPPPH KKSRWKYHYE VTDTLHMTSK RKTPPPNNLT MFTGNAYMVA
310 320 330 340 350
SRDFIEHVFS NSKARQLIEW VKDTYSPDEH LWATLQRASW MPGSDPLHRK
360 370 380 390 400
FDLSDMRAIA RLTKWYDHEG DIENGAPYTS CSGIHQRAVC VYGSGDLHWI
410 420 430
LQNHHLLANK FDPKVDDNVL QCLEEYLRHK AIYGTEL
Length:437
Mass (Da):50,698
Last modified:May 29, 2007 - v2
Checksum:iFF0C1EC8F25320F1
GO

Sequence cautioni

The sequence AAH18297.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB25548.1 differs from that shown. Reason: Erroneous termination at position 35. Translated as Cys.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti101 – 1011A → G in AAK72480. 1 PublicationCurated
Sequence conflicti353 – 3531L → M in BAB25548. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY039027 mRNA. Translation: AAK72480.1.
EF202835 mRNA. Translation: ABM91120.1.
AK008234 mRNA. Translation: BAB25548.1. Sequence problems.
BC018297 mRNA. Translation: AAH18297.1. Different initiation.
CCDSiCCDS40678.1.
RefSeqiNP_082363.2. NM_028087.2.
XP_006511526.1. XM_006511463.1.
XP_006511527.1. XM_006511464.1.
XP_006511528.1. XM_006511465.1.
UniGeneiMm.195555.

Genome annotation databases

EnsembliENSMUST00000034751; ENSMUSP00000034751; ENSMUSG00000032226.
GeneIDi72077.
KEGGimmu:72077.
UCSCiuc009qnt.1. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

core 2 beta 6 GlcNAc T3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY039027 mRNA. Translation: AAK72480.1 .
EF202835 mRNA. Translation: ABM91120.1 .
AK008234 mRNA. Translation: BAB25548.1 . Sequence problems.
BC018297 mRNA. Translation: AAH18297.1 . Different initiation.
CCDSi CCDS40678.1.
RefSeqi NP_082363.2. NM_028087.2.
XP_006511526.1. XM_006511463.1.
XP_006511527.1. XM_006511464.1.
XP_006511528.1. XM_006511465.1.
UniGenei Mm.195555.

3D structure databases

ProteinModelPortali Q5JCT0.
SMRi Q5JCT0. Positions 67-432.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT14. Glycosyltransferase Family 14.

PTM databases

PhosphoSitei Q5JCT0.

Proteomic databases

MaxQBi Q5JCT0.
PRIDEi Q5JCT0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034751 ; ENSMUSP00000034751 ; ENSMUSG00000032226 .
GeneIDi 72077.
KEGGi mmu:72077.
UCSCi uc009qnt.1. mouse.

Organism-specific databases

CTDi 9245.
MGIi MGI:1919327. Gcnt3.

Phylogenomic databases

eggNOGi NOG253565.
GeneTreei ENSGT00760000119183.
HOGENOMi HOG000293251.
HOVERGENi HBG051711.
InParanoidi Q5JCT0.
KOi K09662.
OMAi MTADCEH.
OrthoDBi EOG74N5HW.
PhylomeDBi Q5JCT0.
TreeFami TF315534.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_198517. O-linked glycosylation of mucins.

Miscellaneous databases

ChiTaRSi Gcnt3. mouse.
NextBioi 335378.
PROi Q5JCT0.
SOURCEi Search...

Gene expression databases

Bgeei Q5JCT0.
CleanExi MM_GCNT3.
Genevestigatori Q5JCT0.

Family and domain databases

InterProi IPR003406. Glyco_trans_14.
[Graphical view ]
Pfami PF02485. Branch. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Core 2 beta-1,6-N-acetylglucosaminyltransferase II (Core2-GlcNAcT-II)."
    Yeh J.-C., Ong E., Fukuda M.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Mucin biosynthesis: molecular cloning and expression of mouse mucus-type core 2 beta-1,6 N-acetylglucosaminyltransferase."
    Hashimoto M., Tan S., Mori N., Cheng H., Cheng P.-W.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Colon.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Small intestine.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.

Entry informationi

Entry nameiGCNT3_MOUSE
AccessioniPrimary (citable) accession number: Q5JCT0
Secondary accession number(s): A2TIK7, Q8VCX9, Q9D8A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: November 26, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3