Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3

Gene

B3gnt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides. Also acts as a core1-1,3-N-acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6-sulfo sialyl Lewis x on extended core1 O-glycans.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->3)-(N-acetyl-D-glucosaminyl-(1->6))-N-acetyl-D-galactosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-(N-acetyl-D-glucosaminyl-(1->6))-N-acetyl-D-galactosaminyl-R.By similarity
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.
R-MMU-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3 (EC:2.4.1.149By similarity)
Alternative name(s):
Core 1 extending beta-1,3-N-acetylglucosaminyltransferaseBy similarity (EC:2.4.1.146By similarity)
Core1-beta3GlcNAcT
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
Short name:
BGnT-3
Short name:
Beta-1,3-Gn-T3
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 3
Short name:
Beta3Gn-T3
Gene namesi
Name:B3gnt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2152535. B3gnt3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10CytoplasmicSequence analysis10
Transmembranei11 – 31Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini32 – 372LumenalSequence analysisAdd BLAST341

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191731 – 372N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3Add BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi64N-linked (GlcNAc...)Sequence analysis1
Glycosylationi184N-linked (GlcNAc...)Sequence analysis1
Glycosylationi202N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ5JCS9.
PaxDbiQ5JCS9.
PRIDEiQ5JCS9.

PTM databases

PhosphoSitePlusiQ5JCS9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031803.
GenevisibleiQ5JCS9. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034260.

Structurei

3D structure databases

ProteinModelPortaliQ5JCS9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000232195.
HOVERGENiHBG050653.
InParanoidiQ5JCS9.
KOiK07970.
OMAiYKHCRDF.
OrthoDBiEOG091G0AXM.
PhylomeDBiQ5JCS9.
TreeFamiTF318639.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5JCS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLPRQSPYE ILLLVLVALL VLLLLLTSKS PPSCSAPERS KEPEDNPGWA
60 70 80 90 100
TGHPRTHPAR CRANLSVSSH PDFAGLPLHV RDFLFYRHCR DFPVLREPRV
110 120 130 140 150
TKCAEPVFLL LAIKSSPANY GRRQMLRTTW ARERRVRGAP LRRLFLVGSD
160 170 180 190 200
RDPQQARKYN RLLELEAQKY GDILQWDFHD SFFNLTLKQV LFLEWQLTYC
210 220 230 240 250
TNASFVLNGD DDVFAHTDNM VTYLQDHDPD QHLFVGHLIQ NVGPIRVPWS
260 270 280 290 300
KYFIPALVMA EDRYPPYCGG GGFLLSRFTV AALRRAARVL PMFPIDDVFL
310 320 330 340 350
GMCLQQQGLA PGTHSGVRTA GVFPPSPRVS SFDPCFYRDL LLVHRFLPFE
360 370
MLLMWDALNQ PQLLCGRQSP AY
Length:372
Mass (Da):42,586
Last modified:February 15, 2005 - v1
Checksum:i484771C8054DE265
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256A → T in AAH26418 (PubMed:15489334).Curated1
Sequence conflicti283L → I in AAK68855 (Ref. 1) Curated1
Sequence conflicti283L → I in BAB25824 (PubMed:16141072).Curated1
Sequence conflicti295I → V in AAK68855 (Ref. 1) Curated1
Sequence conflicti295I → V in BAB25824 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037785 mRNA. Translation: AAK68855.1.
AY039028 mRNA. Translation: AAK72481.1.
AK008674 mRNA. Translation: BAB25824.1.
BC026418 mRNA. Translation: AAH26418.1.
CCDSiCCDS22405.1.
RefSeqiNP_082465.3. NM_028189.3.
XP_006509815.1. XM_006509752.3.
XP_006509816.1. XM_006509753.3.
UniGeneiMm.290837.

Genome annotation databases

EnsembliENSMUST00000034260; ENSMUSP00000034260; ENSMUSG00000031803.
GeneIDi72297.
KEGGimmu:72297.
UCSCiuc009mer.3. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

beta 3 GlcNAc-T III

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY037785 mRNA. Translation: AAK68855.1.
AY039028 mRNA. Translation: AAK72481.1.
AK008674 mRNA. Translation: BAB25824.1.
BC026418 mRNA. Translation: AAH26418.1.
CCDSiCCDS22405.1.
RefSeqiNP_082465.3. NM_028189.3.
XP_006509815.1. XM_006509752.3.
XP_006509816.1. XM_006509753.3.
UniGeneiMm.290837.

3D structure databases

ProteinModelPortaliQ5JCS9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034260.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

PTM databases

PhosphoSitePlusiQ5JCS9.

Proteomic databases

MaxQBiQ5JCS9.
PaxDbiQ5JCS9.
PRIDEiQ5JCS9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034260; ENSMUSP00000034260; ENSMUSG00000031803.
GeneIDi72297.
KEGGimmu:72297.
UCSCiuc009mer.3. mouse.

Organism-specific databases

CTDi10331.
MGIiMGI:2152535. B3gnt3.

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000232195.
HOVERGENiHBG050653.
InParanoidiQ5JCS9.
KOiK07970.
OMAiYKHCRDF.
OrthoDBiEOG091G0AXM.
PhylomeDBiQ5JCS9.
TreeFamiTF318639.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.
R-MMU-913709. O-linked glycosylation of mucins.

Miscellaneous databases

PROiQ5JCS9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031803.
GenevisibleiQ5JCS9. MM.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB3GN3_MOUSE
AccessioniPrimary (citable) accession number: Q5JCS9
Secondary accession number(s): Q8R0U2, Q9D7Z2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.