Q5J8M3 (EMC4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ER membrane protein complex subunit 4

Alternative name(s):
Cell proliferation-inducing gene 17 protein
Transmembrane protein 85

Gene names

Name:	EMC4
Synonyms:	TMEM85
ORF Names:	HSPC184, PIG17

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May mediate anti-apoptotic activity. Ref.8
Subunit structure	Component of the ER membrane protein complex (EMC). Ref.11
Subcellular location	Membrane; Multi-pass membrane protein Ref.11.
Tissue specificity	Isoform 1 is expressed in brain and heart. Isoform 2 is expressed in heart. Ref.8
Sequence similarities	Belongs to the EMC4 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ER membrane protein complex Inferred from direct assay Ref.11. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5J8M3-1)

Also known as: TMEM85v1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5J8M3-2)

Also known as: TMEM85v2;

The sequence of this isoform differs from the canonical sequence as follows:
     119-149: TFKMLESSSQKFLQGLVYLIGNLMGLALAVY → KNGVQWWRTAFVNMRKQRLVPMYLGLIYILL
     150-183: Missing.

Isoform 3 (identifier: Q5J8M3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     68-136: RCWDIALGPL...SQKFLQGLVY → VQRYRCYRSP...KNRRCLEHKV
     137-183: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	ER membrane protein complex subunit 4		PRO_0000251914
Regions
	Transmembrane	84 – 104	21	Helical; Potential
	Transmembrane	130 – 150	21	Helical; Potential
Natural variations
	Alternative sequence	68 – 136	69	RCWDI…QGLVY → VQRYRCYRSPWASPELHTHP LLLGGVNVDSSYPEDLETKL LEAGISVFYISATKVLNNLK NRRCLEHKV in isoform 3.		VSP_037374
	Alternative sequence	119 – 149	31	TFKML…ALAVY → KNGVQWWRTAFVNMRKQRLV PMYLGLIYILL in isoform 2.		VSP_020798
	Alternative sequence	137 – 183	47	Missing in isoform 3.		VSP_037375
	Alternative sequence	150 – 183	34	Missing in isoform 2.		VSP_020799
	Natural variant	98	1	P → T. Corresponds to variant rs11544437 [ dbSNP | Ensembl ].		VAR_053775
Experimental info
	Sequence conflict	47	1	Y → H in AAR24622. Ref.1
	Sequence conflict	129	1	K → R in AAR24622. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (TMEM85v1) [UniParc]. Last modified October 3, 2006. Version 2. Checksum: 96888D46FF22DEDE	FASTA	183	20,087
	10 20 30 40 50 60 MTAQGGLVAN RGRRFKWAIE LSGPGGGSRG RSDRGSGQGD SLYPVGYLDK QVPDTSVQET 70 80 90 100 110 120 DRILVEKRCW DIALGPLKQI PMNLFIMYMA GNTISIFPTM MVCMMAWRPI QALMAISATF 130 140 150 160 170 180 KMLESSSQKF LQGLVYLIGN LMGLALAVYK CQSMGLLPTH ASDWLAFIEP PERMEFSGGG LLL
	Isoform 2 (TMEM85v2) [UniParc]. Checksum: D23EDF429CD88EBF	FASTA	149	16,745
	10 20 30 40 50 60 MTAQGGLVAN RGRRFKWAIE LSGPGGGSRG RSDRGSGQGD SLYPVGYLDK QVPDTSVQET 70 80 90 100 110 120 DRILVEKRCW DIALGPLKQI PMNLFIMYMA GNTISIFPTM MVCMMAWRPI QALMAISAKN 130 140 GVQWWRTAFV NMRKQRLVPM YLGLIYILL
	Isoform 3 [UniParc]. Checksum: A76B65D99C017AF7	FASTA	136	15,088
	10 20 30 40 50 60 MTAQGGLVAN RGRRFKWAIE LSGPGGGSRG RSDRGSGQGD SLYPVGYLDK QVPDTSVQET 70 80 90 100 110 120 DRILVEKVQR YRCYRSPWAS PELHTHPLLL GGVNVDSSYP EDLETKLLEA GISVFYISAT 130 KVLNNLKNRR CLEHKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a proliferation-inducing gene." Kim J.W. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Umbilical cord blood.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Brain, Placenta and Thalamus.
[4]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"Transmembrane protein 85 from both human (TMEM85) and yeast (YGL231c) inhibit hydrogen peroxide mediated cell death in yeast." Ring G., Khoury C.M., Solar A.J., Yang Z., Mandato C.A., Greenwood M.T. FEBS Lett. 582:2637-2642(2008) [PubMed] [Europe PMC] [Abstract] Cited for: POSSIBLE FUNCTION, TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"Defining human ERAD networks through an integrative mapping strategy." Christianson J.C., Olzmann J.A., Shaler T.A., Sowa M.E., Bennett E.J., Richter C.M., Tyler R.E., Greenblatt E.J., Harper J.W., Kopito R.R. Nat. Cell Biol. 14:93-105(2012) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE EMC COMPLEX, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY336092 mRNA. Translation: AAR24622.1. AF151018 mRNA. Translation: AAF36104.1. AK075227 mRNA. Translation: BAC11486.1. AK290524 mRNA. Translation: BAF83213.1. AK296184 mRNA. Translation: BAG58916.1. AC079203 Genomic DNA. No translation available. CH471125 Genomic DNA. Translation: EAW92292.1. BC002583 mRNA. Translation: AAH02583.1.
IPI	IPI00009320. IPI00031499. IPI00791782.
RefSeq	NP_057538.1. NM_016454.2.
UniGene	Hs.250905.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5J8M3. 1 interaction.
STRING	9606.ENSP00000267750.
PTM databases
PhosphoSite	Q5J8M3.
Polymorphism databases
DMDM	115502869.
Proteomic databases
PaxDb	Q5J8M3.
PeptideAtlas	Q5J8M3.
PRIDE	Q5J8M3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000249209; ENSP00000249209; ENSG00000128463. ENST00000267750; ENSP00000267750; ENSG00000128463.
GeneID	51234.
KEGG	hsa:51234.
UCSC	uc001zhq.3. human. uc001zhs.3. human. uc010ucb.1. human.
Organism-specific databases
CTD	51234.
GeneCards	GC15P034519.
HGNC	HGNC:28032. EMC4.
HPA	HPA053440.
neXtProt	NX_Q5J8M3.
PharmGKB	PA143485634.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG308755.
HOGENOM	HOG000163282.
HOVERGEN	HBG056123.
InParanoid	Q5J8M3.
OMA	KSWDLAL.
OrthoDB	EOG405S2H.
PhylomeDB	Q5J8M3.
Gene expression databases
ArrayExpress	Q5J8M3.
Bgee	Q5J8M3.
CleanEx	HS_TMEM85.
Genevestigator	Q5J8M3.
GermOnline	ENSG00000128463. Homo sapiens.
Family and domain databases
InterPro	IPR009445. DUF1077_TMEM85. [Graphical view]
PANTHER	PTHR19315. PTHR19315. 1 hit.
Pfam	PF06417. DUF1077. 1 hit. [Graphical view]
PIRSF	PIRSF017207. UCP017207_TM-p85. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	51234.
NextBio	54345.

Entry information

Entry name

EMC4_HUMAN

Accession

Primary (citable) accession number: Q5J8M3
Secondary accession number(s): A8K3A9 , B4DJQ4, Q96KX9, Q9BUI5, Q9P0T9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	October 3, 2006
Last modified:	May 1, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.