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Protein

Mitogen-activated protein kinase 2

Gene

MPK2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei90ATPPROSITE-ProRule annotation1
Active sitei187Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi67 – 75ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-110056 MAPK3 (ERK1) activation
R-OSA-112409 RAF-independent MAPK1/3 activation
R-OSA-112411 MAPK1 (ERK2) activation
R-OSA-198753 ERK/MAPK targets
R-OSA-198765 Signalling to ERK5
R-OSA-202670 ERKs are inactivated
R-OSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-OSA-3371453 Regulation of HSF1-mediated heat shock response
R-OSA-375165 NCAM signaling for neurite out-growth
R-OSA-442742 CREB phosphorylation through the activation of Ras
R-OSA-444257 RSK activation
R-OSA-445144 Signal transduction by L1
R-OSA-5673001 RAF/MAP kinase cascade
R-OSA-5674135 MAP2K and MAPK activation
R-OSA-5674499 Negative feedback regulation of MAPK pathway
R-OSA-6798695 Neutrophil degranulation
R-OSA-881907 Gastrin-CREB signalling pathway via PKC and MAPK

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 2 (EC:2.7.11.24)
Short name:
MAP kinase 2
Gene namesi
Name:MPK2
Ordered Locus Names:Os08g0157000, LOC_Os08g06060
ORF Names:P0438H08.24
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002397451 – 394Mitogen-activated protein kinase 2Add BLAST394

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei221PhosphotyrosineBy similarity1

Post-translational modificationi

The phosphorylation on Tyr-221 activates the enzyme (By similarity). A conserved Thr, which must also be phosphorylated to activate the enzyme in closely related sequences, is replaced by Met-219 in this sequence.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5J4W4
PRIDEiQ5J4W4

Expressioni

Inductioni

By infection with rice blast fungus (M.grisea).1 Publication

Gene expression databases

ExpressionAtlasiQ5J4W4 baseline and differential
GenevisibleiQ5J4W4 OS

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os08g06060.1

Structurei

3D structure databases

ProteinModelPortaliQ5J4W4
SMRiQ5J4W4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 347Protein kinasePROSITE-ProRule annotationAdd BLAST287

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 42Gly-richAdd BLAST38

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
HOGENOMiHOG000233024
InParanoidiQ5J4W4
KOiK20600
OMAiMSSGAMD
OrthoDBiEOG09360BXQ

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00219 TyrKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Q5J4W4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMEGGGGGG HGHHGGGGGG HGHHGGIGGG EAQIKGTLTH GGRYVQYNVY
60 70 80 90 100
GNLFEVSSKY VPPIRPVGRG ACGIICAVVN AQTRQEVAIK KIGNAFDNQI
110 120 130 140 150
DAKRTLREIK LLRHMDHDNV ISIKDIIRPP RRENFNDVYI VYELMDTDLH
160 170 180 190 200
HLLRSNQPLT DDHCQYFLYQ VLRGLKYVHS ANVLHRDLRP SNLLLNAKCD
210 220 230 240 250
LKIGDFGLAR TTNETDFMME YVVTRWYRAP ELLLNCSEYT AAIDIWSVGC
260 270 280 290 300
ILGEIVTREP LFPGKDYVHQ LRLITELIGS PDDSSLGFLR SDNARRYVRS
310 320 330 340 350
LPQYPKQQFR ARFPTMSSGA MDLLERMLVF DPSKRITVDE ALCHPYLASL
360 370 380 390
HEIYDEPVCP APFSFDFEQP SLTEEDIKEI IWREALKFNP EPIH
Length:394
Mass (Da):44,585
Last modified:February 15, 2005 - v1
Checksum:iAC224C9134227481
GO

Sequence cautioni

The sequence BAC99508 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY506572 mRNA Translation: AAS79349.1
AP004460 Genomic DNA Translation: BAC99508.1 Different initiation.
AP008214 Genomic DNA Translation: BAF22942.2
AP014964 Genomic DNA Translation: BAT03900.1
RefSeqiXP_015650444.1, XM_015794958.1

Genome annotation databases

EnsemblPlantsiOs08t0157000-00; Os08t0157000-00; Os08g0157000
GeneIDi4344698
GrameneiOs08t0157000-00; Os08t0157000-00; Os08g0157000
KEGGiosa:4344698

Similar proteinsi

Entry informationi

Entry nameiMPK2_ORYSJ
AccessioniPrimary (citable) accession number: Q5J4W4
Secondary accession number(s): Q0J7X3, Q6ZD93
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: February 15, 2005
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health