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Protein

Mitogen-activated protein kinase 2

Gene

MPK2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901ATPPROSITE-ProRule annotation
Active sitei187 – 1871Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi67 – 759ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_291732. Regulation of HSF1-mediated heat shock response.
REACT_299507. ISG15 antiviral mechanism.
REACT_301619. ERK2 activation.
REACT_303121. ERKs are inactivated.
REACT_303489. ERK1 activation.
REACT_310298. Signal transduction by L1.
REACT_328758. Signaling by FGFR.
REACT_331265. CREB phosphorylation through the activation of Ras.
REACT_343002. ERK/MAPK targets.
REACT_343884. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 2 (EC:2.7.11.24)
Short name:
MAP kinase 2
Gene namesi
Name:MPK2
Ordered Locus Names:Os08g0157000, LOC_Os08g06060
ORF Names:P0438H08.24
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 8

Organism-specific databases

GrameneiQ5J4W4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Mitogen-activated protein kinase 2PRO_0000239745Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei221 – 2211PhosphotyrosineBy similarity

Post-translational modificationi

The phosphorylation on Tyr-221 activates the enzyme (By similarity). A conserved Thr, which must also be phosphorylated to activate the enzyme in closely related sequences, is replaced by Met-219 in this sequence.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

By infection with rice blast fungus (M.grisea).1 Publication

Gene expression databases

ExpressionAtlasiQ5J4W4. baseline.

Structurei

3D structure databases

SMRiQ5J4W4. Positions 49-391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 347287Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 4238Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ5J4W4.
KOiK04371.
OMAiMSSGAMD.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5J4W4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMEGGGGGG HGHHGGGGGG HGHHGGIGGG EAQIKGTLTH GGRYVQYNVY
60 70 80 90 100
GNLFEVSSKY VPPIRPVGRG ACGIICAVVN AQTRQEVAIK KIGNAFDNQI
110 120 130 140 150
DAKRTLREIK LLRHMDHDNV ISIKDIIRPP RRENFNDVYI VYELMDTDLH
160 170 180 190 200
HLLRSNQPLT DDHCQYFLYQ VLRGLKYVHS ANVLHRDLRP SNLLLNAKCD
210 220 230 240 250
LKIGDFGLAR TTNETDFMME YVVTRWYRAP ELLLNCSEYT AAIDIWSVGC
260 270 280 290 300
ILGEIVTREP LFPGKDYVHQ LRLITELIGS PDDSSLGFLR SDNARRYVRS
310 320 330 340 350
LPQYPKQQFR ARFPTMSSGA MDLLERMLVF DPSKRITVDE ALCHPYLASL
360 370 380 390
HEIYDEPVCP APFSFDFEQP SLTEEDIKEI IWREALKFNP EPIH
Length:394
Mass (Da):44,585
Last modified:February 15, 2005 - v1
Checksum:iAC224C9134227481
GO

Sequence cautioni

The sequence BAC99508.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY506572 mRNA. Translation: AAS79349.1.
AP004460 Genomic DNA. Translation: BAC99508.1. Different initiation.
RefSeqiNP_001061028.2. NM_001067563.2.
UniGeneiOs.16795.

Genome annotation databases

EnsemblPlantsiOS08T0157000-00; OS08T0157000-00; OS08G0157000.
GeneIDi4344698.
KEGGiosa:4344698.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY506572 mRNA. Translation: AAS79349.1.
AP004460 Genomic DNA. Translation: BAC99508.1. Different initiation.
RefSeqiNP_001061028.2. NM_001067563.2.
UniGeneiOs.16795.

3D structure databases

SMRiQ5J4W4. Positions 49-391.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS08T0157000-00; OS08T0157000-00; OS08G0157000.
GeneIDi4344698.
KEGGiosa:4344698.

Organism-specific databases

GrameneiQ5J4W4.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ5J4W4.
KOiK04371.
OMAiMSSGAMD.

Enzyme and pathway databases

ReactomeiREACT_291732. Regulation of HSF1-mediated heat shock response.
REACT_299507. ISG15 antiviral mechanism.
REACT_301619. ERK2 activation.
REACT_303121. ERKs are inactivated.
REACT_303489. ERK1 activation.
REACT_310298. Signal transduction by L1.
REACT_328758. Signaling by FGFR.
REACT_331265. CREB phosphorylation through the activation of Ras.
REACT_343002. ERK/MAPK targets.
REACT_343884. Signalling to ERK5.

Gene expression databases

ExpressionAtlasiQ5J4W4. baseline.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel protein kinase OsMAPK-like involved in the developmental regulation for rice plants."
    Wang Y.D., Chen X., Ye Z.
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
    Reyna N.S., Yang Y.
    Mol. Plant Microbe Interact. 19:530-540(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, NOMENCLATURE.

Entry informationi

Entry nameiMPK2_ORYSJ
AccessioniPrimary (citable) accession number: Q5J4W4
Secondary accession number(s): Q6ZD93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: February 15, 2005
Last modified: April 1, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.