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Q5J4W4 (MPK2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 2

Short name=MAP kinase 2
EC=2.7.11.24
Gene names
Name:MPK2
Ordered Locus Names:Os08g0157000, LOC_Os08g06060
ORF Names:P0438H08.24
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Induction

By infection with rice blast fungus (M.grisea). Ref.3

Post-translational modification

The phosphorylation on Tyr-221 activates the enzyme By similarity. A conserved Thr, which must also be phosphorylated to activate the enzyme in closely related sequences, is replaced by Met-219 in this sequence.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence BAC99508.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcortical microtubule organization

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

hyperosmotic response

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

hypotonic salinity response

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

jasmonic acid and ethylene-dependent systemic resistance

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

male meiosis cytokinesis

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

pollen development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to abscisic acid

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to cold

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to fungus

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to indolebutyric acid

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

systemic acquired resistance, salicylic acid mediated signaling pathway

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentcytosol

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

protein tyrosine kinase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394Mitogen-activated protein kinase 2
PRO_0000239745

Regions

Domain61 – 347287Protein kinase
Nucleotide binding67 – 759ATP By similarity
Compositional bias5 – 4238Gly-rich

Sites

Active site1871Proton acceptor By similarity
Binding site901ATP By similarity

Amino acid modifications

Modified residue2211Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5J4W4 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: AC224C9134227481

FASTA39444,585
        10         20         30         40         50         60 
MRMEGGGGGG HGHHGGGGGG HGHHGGIGGG EAQIKGTLTH GGRYVQYNVY GNLFEVSSKY 

        70         80         90        100        110        120 
VPPIRPVGRG ACGIICAVVN AQTRQEVAIK KIGNAFDNQI DAKRTLREIK LLRHMDHDNV 

       130        140        150        160        170        180 
ISIKDIIRPP RRENFNDVYI VYELMDTDLH HLLRSNQPLT DDHCQYFLYQ VLRGLKYVHS 

       190        200        210        220        230        240 
ANVLHRDLRP SNLLLNAKCD LKIGDFGLAR TTNETDFMME YVVTRWYRAP ELLLNCSEYT 

       250        260        270        280        290        300 
AAIDIWSVGC ILGEIVTREP LFPGKDYVHQ LRLITELIGS PDDSSLGFLR SDNARRYVRS 

       310        320        330        340        350        360 
LPQYPKQQFR ARFPTMSSGA MDLLERMLVF DPSKRITVDE ALCHPYLASL HEIYDEPVCP 

       370        380        390 
APFSFDFEQP SLTEEDIKEI IWREALKFNP EPIH 

« Hide

References

« Hide 'large scale' references
[1]"A novel protein kinase OsMAPK-like involved in the developmental regulation for rice plants."
Wang Y.D., Chen X., Ye Z.
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
Reyna N.S., Yang Y.
Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY506572 mRNA. Translation: AAS79349.1.
AP004460 Genomic DNA. Translation: BAC99508.1. Different initiation.
RefSeqNP_001061028.2. NM_001067563.2.
UniGeneOs.16795.

3D structure databases

ProteinModelPortalQ5J4W4.
SMRQ5J4W4. Positions 49-391.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS08T0157000-00; OS08T0157000-00; OS08G0157000.
GeneID4344698.
KEGGosa:4344698.

Organism-specific databases

GrameneQ5J4W4.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
KOK04371.
OMAWREALKF.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK2_ORYSJ
AccessionPrimary (citable) accession number: Q5J4W4
Secondary accession number(s): Q6ZD93
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: February 15, 2005
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations