Reviewed, UniProtKB/Swiss-Prot Q5IZM4 (CP51_MYCVP)

Names and origin

Protein names

Recommended name:
    Lanosterol 14-alpha demethylase
    EC=1.14.13.70
Alternative name(s):
    Cytochrome P450 51
    CYPLI
    P450-LIA1
    Sterol 14-alpha demethylase
    P450-14DM

Gene names

Name:	cyp51
Ordered Locus Names:	Mvan_5161

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Its precise biological substrate is not known. Catalyzes C14-demethylation of lanosterol, 24,25-dihydrolanosterol and obtusifoliol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Lanosterol 14-alpha demethylase		PRO_0000052018
Sites
	Metal binding	395	1	Iron (heme axial ligand)

Sequences

	Sequence		Length	Mass (Da)
	Q5IZM4-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 716ED23C210A2ADC	FASTA	452	51,681
	10 20 30 40 50 60 MTAVKEVPRV SGGEEEHGHL EEFRTDPIGL MKRVREECGD VGWFQLADKQ VILLSGAEAN 70 80 90 100 110 120 EFFFRSSDSE LNQAEAYPFM TPIFGEGVVF DADPERRAEM LHNTALRGEH MKGHATTIEA 130 140 150 160 170 180 EVRKMIEGWG ESGEIDLLEF FAELTIYTST ACLIGLKFRN QLDSRFANYY HLLERGTDPL 190 200 210 220 230 240 CYVDPYLPIE SFRIRDEARA GLVELVQDVM HGRIANPPKD KSDRDMLDVL VSIKDEDGNP 250 260 270 280 290 300 RFTANEITGM FISLMFAGHH TSSGTSSWTL IELLRHPEFY AKVQQELDDL YADGQEVSFH 310 320 330 340 350 360 ALRQIPSLDN ALKETLRLHP PLIILMRVAQ DEFEVAGYPI HKGQMVAASP AISNRIPEDF 370 380 390 400 410 420 PNPDDFDPDR YEKPRQEDLI NRWTWIPFGA GKHRCVGAAF AQMQIKAIFS VLLREYEFEM 430 440 450 AQPPESYQND HSKMVVQLAR PAKVRYRRRV RD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cytochromes p450 from PAH-degrading strain Mycobacterium vanbaalenii PYR-1." Brezna B., Stingley R.L., Freeman J.P., Khan A.A., Cerniglia C.E. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Mycobacterium vanbaalenii PYR-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY575951 Genomic DNA. Translation: AAT40578.1. CP000511 Genomic DNA. Translation: ABM15932.1.
RefSeq	YP_955938.1.
3D structure databases
SMR	Q5IZM4. Positions 7-449.
ModBase	Search...
Genome annotation databases
GeneID	4647445.
GenomeReviews	Gene locus Mvan_5161 in contig CP000511_GR.
KEGG	mva:Mvan_5161.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q5IZM4. MEQVTGG.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_MYCVP

Accession

Primary (citable) accession number: Q5IZM4
Secondary accession number(s): A1TFI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	February 15, 2005
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)