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Q5IGR8

- EXT1A_DANRE

UniProt

Q5IGR8 - EXT1A_DANRE

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Protein

Exostosin-1a

Gene

ext1a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

Manganese.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei502 – 5021SubstrateBy similarity
Metal bindingi551 – 5511Manganese; catalyticBy similarity
Binding sitei579 – 5791SubstrateBy similarity
Active sitei638 – 6381By similarity

GO - Molecular functioni

  1. glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Source: UniProtKB
  2. N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. heparan sulfate proteoglycan biosynthetic process Source: UniProtKB
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_226880. HS-GAG biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-1a (EC:2.4.1.224, EC:2.4.1.225)
Alternative name(s):
Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase 1a
Multiple exostoses protein 1 homolog a
Gene namesi
Name:ext1a
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 16

Organism-specific databases

ZFINiZDB-GENE-050211-3. ext1a.

Subcellular locationi

GO - Cellular componenti

  1. integral component of endoplasmic reticulum membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 730730Exostosin-1aPRO_0000149659Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi636 ↔ 688By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Developmental stagei

Expressed both maternally and zygotically. During gastrulation, expressed in the neuroectoderm. At the 3-somite stage, expressed in the prospective forebrain, hindbrain and tailbud. From the 5-somite stage, expressed in the somitic mesoderm. At the 16-somite stage, also expressed in the dorsal neural tube, the eye and the Kupffer's vesicle. At 48 hours-post-fertilization (hpf), expression continues in the dorsal diencephalon and cerebellum and is observed in the otic vesicle.1 Publication

Gene expression databases

BgeeiQ5IGR8.

Structurei

3D structure databases

ProteinModelPortaliQ5IGR8.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Topological domaini28 – 730703LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni528 – 5336Substrate bindingBy similarity
Regioni549 – 5513Substrate bindingBy similarity
Regioni634 – 6385Substrate bindingBy similarity
Regioni672 – 68514Substrate bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG269038.
GeneTreeiENSGT00550000074496.
HOGENOMiHOG000266990.
HOVERGENiHBG003459.
InParanoidiQ5IGR8.
OMAiQTETEDY.
OrthoDBiEOG7RBZ7Z.
PhylomeDBiQ5IGR8.
TreeFamiTF314231.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004263. Exostosin.
IPR027670. Exostosin-1.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11062. PTHR11062. 1 hit.
PTHR11062:SF45. PTHR11062:SF45. 1 hit.
PfamiPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5IGR8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQAKKRYLIL FSAGVCLILL FYLQGPASRR TPKRGDDPHP HWPHFSDPLR
60 70 80 90 100
AFIPWDQTET EDYNVRASPR HKRDDSTGAD KCRMDSCFDF ELCKRNGFKV
110 120 130 140 150
YVYPQQKGEK ISESYQNILS SIEGSRFYTS DPGQACLFVL NLDTLDRDQL
160 170 180 190 200
SPQYVHNLKT KIQNLNLWNN GRNHLIFNLY SGTWPDYTED LGFDIGQAML
210 220 230 240 250
AKASISTESF RPNFDISIPL FSKDHPRTGG ERGFLKYNTI PPFRKYMLVF
260 270 280 290 300
KGKRYLTGIG SDTRNALYHI HNAEDVVLLT TCKHGKDWQK HKDARCDKDN
310 320 330 340 350
AEYDRYDYKE MLHNSTFCLV PRGRRLGSFR FLEALQAACV PVMLSNGWEL
360 370 380 390 400
PFSEIIDWRT AAVIGDERLL LQIPSTVRSI HQDRILSLRQ QTQFLWEAYF
410 420 430 440 450
SSVEKIVLTT LEIIQDRVLQ HSAHSTLMWN RLPGGLFTLP QYSSYLGDFP
460 470 480 490 500
FFYALLGIKP HQKFTAVIHA VTPLVSQSQP IFKLLVAVAK SQFCAQIMVL
510 520 530 540 550
WNCDKPLPSK HRWPATSVPV IVIEGESKVM SSRFLPYENI ITDAVLSLDE
560 570 580 590 600
DTVLSTTEVD FAFTVWQSFP ERIVGYPARS HFWDSNKERW GYTSKWTNDY
610 620 630 640 650
SMVLTGAAFY HRYYNYLYTH YLPGSLKGLV DQLSNCEDIL MNFLVSAVTK
660 670 680 690 700
MPPIKVTQKK QYKETMMGQT SRASRWADPD HFAQRQTCMN KFASWFGTMP
710 720 730
LVHSQMRLDP VLFRDQVSIL RKKYRDIERL
Length:730
Mass (Da):84,618
Last modified:February 15, 2005 - v1
Checksum:i6BBBF8923E42E49C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY734455 mRNA. Translation: AAW29033.1.
UniGeneiDr.79578.

Genome annotation databases

EnsembliENSDART00000006439; ENSDARP00000003397; ENSDARG00000020373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY734455 mRNA. Translation: AAW29033.1 .
UniGenei Dr.79578.

3D structure databases

ProteinModelPortali Q5IGR8.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000006439 ; ENSDARP00000003397 ; ENSDARG00000020373 .

Organism-specific databases

ZFINi ZDB-GENE-050211-3. ext1a.

Phylogenomic databases

eggNOGi NOG269038.
GeneTreei ENSGT00550000074496.
HOGENOMi HOG000266990.
HOVERGENi HBG003459.
InParanoidi Q5IGR8.
OMAi QTETEDY.
OrthoDBi EOG7RBZ7Z.
PhylomeDBi Q5IGR8.
TreeFami TF314231.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_226880. HS-GAG biosynthesis.

Miscellaneous databases

PROi Q5IGR8.

Gene expression databases

Bgeei Q5IGR8.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR004263. Exostosin.
IPR027670. Exostosin-1.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR11062. PTHR11062. 1 hit.
PTHR11062:SF45. PTHR11062:SF45. 1 hit.
Pfami PF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling."
    Siekmann A.F., Brand M.
    Dev. Dyn. 232:498-505(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiEXT1A_DANRE
AccessioniPrimary (citable) accession number: Q5IGR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 15, 2005
Last modified: October 29, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3