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Q5IGR8

- EXT1A_DANRE

UniProt

Q5IGR8 - EXT1A_DANRE

Protein

Exostosin-1a

Gene

ext1a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Glycosyltransferase required for the biosynthesis of heparan-sulfate.By similarity

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
    UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

    Pathwayi

    GO - Molecular functioni

    1. glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Source: UniProtKB
    2. N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. heparan sulfate proteoglycan biosynthetic process Source: UniProtKB
    2. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_226880. HS-GAG biosynthesis.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT47. Glycosyltransferase Family 47.
    GT64. Glycosyltransferase Family 64.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exostosin-1a (EC:2.4.1.224, EC:2.4.1.225)
    Alternative name(s):
    Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase 1a
    Multiple exostoses protein 1 homolog a
    Gene namesi
    Name:ext1a
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 16

    Organism-specific databases

    ZFINiZDB-GENE-050211-3. ext1a.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of endoplasmic reticulum membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 730730Exostosin-1aPRO_0000149659Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Developmental stagei

    Expressed both maternally and zygotically. During gastrulation, expressed in the neuroectoderm. At the 3-somite stage, expressed in the prospective forebrain, hindbrain and tailbud. From the 5-somite stage, expressed in the somitic mesoderm. At the 16-somite stage, also expressed in the dorsal neural tube, the eye and the Kupffer's vesicle. At 48 hours-post-fertilization (hpf), expression continues in the dorsal diencephalon and cerebellum and is observed in the otic vesicle.1 Publication

    Gene expression databases

    BgeeiQ5IGR8.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5IGR8.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicSequence Analysis
    Topological domaini28 – 730703LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG269038.
    GeneTreeiENSGT00550000074496.
    HOGENOMiHOG000266990.
    HOVERGENiHBG003459.
    InParanoidiQ5IGR8.
    OMAiQTETEDY.
    OrthoDBiEOG7RBZ7Z.
    PhylomeDBiQ5IGR8.
    TreeFamiTF314231.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR004263. Exostosin.
    IPR027670. Exostosin-1.
    IPR015338. HexNAc_Trfase_a.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR11062. PTHR11062. 1 hit.
    PTHR11062:SF45. PTHR11062:SF45. 1 hit.
    PfamiPF03016. Exostosin. 1 hit.
    PF09258. Glyco_transf_64. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5IGR8-1 [UniParc]FASTAAdd to Basket

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    MQAKKRYLIL FSAGVCLILL FYLQGPASRR TPKRGDDPHP HWPHFSDPLR    50
    AFIPWDQTET EDYNVRASPR HKRDDSTGAD KCRMDSCFDF ELCKRNGFKV 100
    YVYPQQKGEK ISESYQNILS SIEGSRFYTS DPGQACLFVL NLDTLDRDQL 150
    SPQYVHNLKT KIQNLNLWNN GRNHLIFNLY SGTWPDYTED LGFDIGQAML 200
    AKASISTESF RPNFDISIPL FSKDHPRTGG ERGFLKYNTI PPFRKYMLVF 250
    KGKRYLTGIG SDTRNALYHI HNAEDVVLLT TCKHGKDWQK HKDARCDKDN 300
    AEYDRYDYKE MLHNSTFCLV PRGRRLGSFR FLEALQAACV PVMLSNGWEL 350
    PFSEIIDWRT AAVIGDERLL LQIPSTVRSI HQDRILSLRQ QTQFLWEAYF 400
    SSVEKIVLTT LEIIQDRVLQ HSAHSTLMWN RLPGGLFTLP QYSSYLGDFP 450
    FFYALLGIKP HQKFTAVIHA VTPLVSQSQP IFKLLVAVAK SQFCAQIMVL 500
    WNCDKPLPSK HRWPATSVPV IVIEGESKVM SSRFLPYENI ITDAVLSLDE 550
    DTVLSTTEVD FAFTVWQSFP ERIVGYPARS HFWDSNKERW GYTSKWTNDY 600
    SMVLTGAAFY HRYYNYLYTH YLPGSLKGLV DQLSNCEDIL MNFLVSAVTK 650
    MPPIKVTQKK QYKETMMGQT SRASRWADPD HFAQRQTCMN KFASWFGTMP 700
    LVHSQMRLDP VLFRDQVSIL RKKYRDIERL 730
    Length:730
    Mass (Da):84,618
    Last modified:February 15, 2005 - v1
    Checksum:i6BBBF8923E42E49C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY734455 mRNA. Translation: AAW29033.1.
    UniGeneiDr.79578.

    Genome annotation databases

    EnsembliENSDART00000006439; ENSDARP00000003397; ENSDARG00000020373.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY734455 mRNA. Translation: AAW29033.1 .
    UniGenei Dr.79578.

    3D structure databases

    ProteinModelPortali Q5IGR8.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT47. Glycosyltransferase Family 47.
    GT64. Glycosyltransferase Family 64.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000006439 ; ENSDARP00000003397 ; ENSDARG00000020373 .

    Organism-specific databases

    ZFINi ZDB-GENE-050211-3. ext1a.

    Phylogenomic databases

    eggNOGi NOG269038.
    GeneTreei ENSGT00550000074496.
    HOGENOMi HOG000266990.
    HOVERGENi HBG003459.
    InParanoidi Q5IGR8.
    OMAi QTETEDY.
    OrthoDBi EOG7RBZ7Z.
    PhylomeDBi Q5IGR8.
    TreeFami TF314231.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_226880. HS-GAG biosynthesis.

    Miscellaneous databases

    PROi Q5IGR8.

    Gene expression databases

    Bgeei Q5IGR8.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR004263. Exostosin.
    IPR027670. Exostosin-1.
    IPR015338. HexNAc_Trfase_a.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR11062. PTHR11062. 1 hit.
    PTHR11062:SF45. PTHR11062:SF45. 1 hit.
    Pfami PF03016. Exostosin. 1 hit.
    PF09258. Glyco_transf_64. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling."
      Siekmann A.F., Brand M.
      Dev. Dyn. 232:498-505(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiEXT1A_DANRE
    AccessioniPrimary (citable) accession number: Q5IGR8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3