Reviewed, UniProtKB/Swiss-Prot Q5IGR8 (EXT1A_DANRE)

Names and origin

Protein names

Recommended name:
    Exostosin-1a
    EC=2.4.1.224
    EC=2.4.1.225
Alternative name(s):
    Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase 1a
    Multiple exostoses protein 1 homolog a

Gene names

Name:

ext1a

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	730 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosyltransferase required for the biosynthesis of heparan-sulfate By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan. UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Developmental stage	Expressed both maternally and zygotically. During gastrulation, expressed in the neuroectoderm. At the 3-somite stage, expressed in the prospective forebrain, hindbrain and tailbud. From the 5-somite stage, expressed in the somitic mesoderm. At the 16-somite stage, also expressed in the dorsal neural tube, the eye and the Kupffer's vesicle. At 48 hours-post-fertilization (hpf), expression continues in the dorsal diencephalon and cerebellum and is observed in the otic vesicle. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 47 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	heparan sulfate proteoglycan biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 730	730	Exostosin-1a		PRO_0000149659
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Signal-anchor for type II membrane protein Potential
	Topological domain	28 – 730	703	Lumenal Potential
Amino acid modifications
	Glycosylation	314	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5IGR8-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 6BBBF8923E42E49C	FASTA	730	84,618
	10 20 30 40 50 60 MQAKKRYLIL FSAGVCLILL FYLQGPASRR TPKRGDDPHP HWPHFSDPLR AFIPWDQTET 70 80 90 100 110 120 EDYNVRASPR HKRDDSTGAD KCRMDSCFDF ELCKRNGFKV YVYPQQKGEK ISESYQNILS 130 140 150 160 170 180 SIEGSRFYTS DPGQACLFVL NLDTLDRDQL SPQYVHNLKT KIQNLNLWNN GRNHLIFNLY 190 200 210 220 230 240 SGTWPDYTED LGFDIGQAML AKASISTESF RPNFDISIPL FSKDHPRTGG ERGFLKYNTI 250 260 270 280 290 300 PPFRKYMLVF KGKRYLTGIG SDTRNALYHI HNAEDVVLLT TCKHGKDWQK HKDARCDKDN 310 320 330 340 350 360 AEYDRYDYKE MLHNSTFCLV PRGRRLGSFR FLEALQAACV PVMLSNGWEL PFSEIIDWRT 370 380 390 400 410 420 AAVIGDERLL LQIPSTVRSI HQDRILSLRQ QTQFLWEAYF SSVEKIVLTT LEIIQDRVLQ 430 440 450 460 470 480 HSAHSTLMWN RLPGGLFTLP QYSSYLGDFP FFYALLGIKP HQKFTAVIHA VTPLVSQSQP 490 500 510 520 530 540 IFKLLVAVAK SQFCAQIMVL WNCDKPLPSK HRWPATSVPV IVIEGESKVM SSRFLPYENI 550 560 570 580 590 600 ITDAVLSLDE DTVLSTTEVD FAFTVWQSFP ERIVGYPARS HFWDSNKERW GYTSKWTNDY 610 620 630 640 650 660 SMVLTGAAFY HRYYNYLYTH YLPGSLKGLV DQLSNCEDIL MNFLVSAVTK MPPIKVTQKK 670 680 690 700 710 720 QYKETMMGQT SRASRWADPD HFAQRQTCMN KFASWFGTMP LVHSQMRLDP VLFRDQVSIL 730 RKKYRDIERL

References

[1]

"Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling." Siekmann A.F., Brand M. Dev. Dyn. 232:498-505(2005) [PubMed: 15614771] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY734455 mRNA. Translation: AAW29033.1.
IPI	IPI00504905.
UniGene	Dr.79578
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5IGR8.
Protein family/group databases
CAZy	GT47. Glycosyltransferase Family 47. GT64. Glycosyltransferase Family 64.
Genome annotation databases
Ensembl	ENSDART00000006439; ENSDARP00000003397; ENSDARG00000020373; Danio rerio. [Genome view]
Organism-specific databases
ZFIN	ZDB-GENE-050211-3. ext1a.
Phylogenomic databases
HOGENOM	Q5IGR8.
HOVERGEN	Q5IGR8.
OMA	PWEQLEN.
Enzyme and pathway databases
BRENDA	2.4.1.224. 96826. 2.4.1.225. 96826.
Gene expression databases
ArrayExpress	Q5IGR8.
Bgee	Q5IGR8.
Family and domain databases
InterPro	IPR004263. Exostosin. IPR015338. HexNAc_Trfase_a. [Graphical view]
Pfam	PF03016. Exostosin. 1 hit. PF09258. Glyco_transf_64. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EXT1A_DANRE

Accession

Primary (citable) accession number: Q5IGR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	February 15, 2005
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project