Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5IGR7 (EXT1B_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exostosin-1b

EC=2.4.1.224
EC=2.4.1.225
Alternative name(s):
Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase 1b
Multiple exostoses protein 1 homolog b
Gene names
Name:ext1b
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length741 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Glycosyltransferase required for the biosynthesis of heparan-sulfate By similarity.

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.

UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.

Developmental stage

Expressed both maternally and zygotically. During gastrulation, expressed in the embryonic midline and in the involuting mesendoderm of the germ ring. During somatogenesis, expressed in the somitic mesoderm. At the 16-somite stage, expression is highest in the tailbud region, the eye and the dorsal somites. At 24 hours-post-fertilization (hpf), expressed in the neural tube, tailbud, posterior somites and forming fin bud. At 48 hpf, expressed in the fin buds and brain. Ref.1

Sequence similarities

Belongs to the glycosyltransferase 47 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 741741Exostosin-1b
PRO_0000149660

Regions

Topological domain1 – 77Cytoplasmic Potential
Transmembrane8 – 2821Helical; Signal-anchor for type II membrane protein; Potential
Topological domain29 – 741713Lumenal Potential

Amino acid modifications

Glycosylation841N-linked (GlcNAc...) Potential
Glycosylation3251N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q5IGR7 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: C2793E62F5378393

FASTA74185,121
        10         20         30         40         50         60 
MQAKKRYLIS LLTGAFLVLL IYLGGGGVPG PAAPGSRSRT HGYNRPDQPW PHFSDPLQHF 

        70         80         90        100        110        120 
SPWDHSDTED YNVHISPRQK RDVNSSVYKG KRCRMQSCFD FSLCQRNGFK VYVYPQQKGE 

       130        140        150        160        170        180 
KISESYQNIL STIEGSRFYT SDPGQACVFV LSLDTLDRDQ LSPQYVHNLK TKVQSLALWN 

       190        200        210        220        230        240 
NGRNHLIFNL YSGTWPDYTE DLGFDIGQAM LAKASISTEN FRPNFDISIP LFSKEHPRTG 

       250        260        270        280        290        300 
GDRGYLKYNT IPPFRKYMLV FKGKRYLTGI GSDTRNALYH VHNAEDVVLL TTCKHGKDWQ 

       310        320        330        340        350        360 
KHKDARCDKD NAEYDKYDYR EMLHNSTFCL VPRGRRLGSF RFLEALQAAC VPVMLSNGWE 

       370        380        390        400        410        420 
LPFSEVIDWN TAAVIGDERL LLQIPSTVRS IHQDKILALR QQTQFLWEAY FSSVEKIVLT 

       430        440        450        460        470        480 
TLEIIQDRVL QQSSRSSVMW NSHPGGLFSL PQYSSYLGDL PFFYAKLGIK PSPKFTAVIH 

       490        500        510        520        530        540 
AVTPLVSQSQ PILKLIVSVA RSQYCAQIIV LWNCDKPLPA KQRWPATAVP IIVIEGENKV 

       550        560        570        580        590        600 
MSSRFQPYES LISDAVLSLD EDTVLSTTEV DFAFTVWQSF PERIVGYPAR SHFWDNNKER 

       610        620        630        640        650        660 
WGYTSKWTND YSMVLTGAAI YHRYYHFLYT HFLPSSLKSM VDQLANCEDI LMNFLVSAVT 

       670        680        690        700        710        720 
KLPPVKVTQK KQYKETMMGQ SSRASRWADP DHFAQRQTCM NKFASWFGGM PLVHSQMRLD 

       730        740 
PVLFKDQVSI LRKKYREIER L 

« Hide

References

[1]"Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling."
Siekmann A.F., Brand M.
Dev. Dyn. 232:498-505(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY734456 mRNA. Translation: AAW29034.1.
UniGeneDr.84943.

3D structure databases

ProteinModelPortalQ5IGR7.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

ZFINZDB-GENE-050211-4. ext1b.

Phylogenomic databases

HOVERGENHBG003459.
PhylomeDBQ5IGR7.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR004263. Exostosin.
IPR027670. Exostosin-1.
IPR015338. HexNAc_Trfase_a.
[Graphical view]
PANTHERPTHR11062. PTHR11062. 1 hit.
PTHR11062:SF7. PTHR11062:SF7. 1 hit.
PfamPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEXT1B_DANRE
AccessionPrimary (citable) accession number: Q5IGR7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 15, 2005
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways