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Q5IGR7

- EXT1B_DANRE

UniProt

Q5IGR7 - EXT1B_DANRE

Protein

Exostosin-1b

Gene

ext1b

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Glycosyltransferase required for the biosynthesis of heparan-sulfate.By similarity

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
    UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

    Pathwayi

    GO - Molecular functioni

    1. glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Source: UniProtKB
    2. N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. heparan sulfate proteoglycan biosynthetic process Source: UniProtKB
    2. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT47. Glycosyltransferase Family 47.
    GT64. Glycosyltransferase Family 64.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exostosin-1b (EC:2.4.1.224, EC:2.4.1.225)
    Alternative name(s):
    Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase 1b
    Multiple exostoses protein 1 homolog b
    Gene namesi
    Name:ext1b
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Unplaced

    Organism-specific databases

    ZFINiZDB-GENE-050211-4. ext1b.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of endoplasmic reticulum membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 741741Exostosin-1bPRO_0000149660Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi325 – 3251N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Developmental stagei

    Expressed both maternally and zygotically. During gastrulation, expressed in the embryonic midline and in the involuting mesendoderm of the germ ring. During somatogenesis, expressed in the somitic mesoderm. At the 16-somite stage, expression is highest in the tailbud region, the eye and the dorsal somites. At 24 hours-post-fertilization (hpf), expressed in the neural tube, tailbud, posterior somites and forming fin bud. At 48 hpf, expressed in the fin buds and brain.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ5IGR7.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77CytoplasmicSequence Analysis
    Topological domaini29 – 741713LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG003459.
    PhylomeDBiQ5IGR7.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR004263. Exostosin.
    IPR027670. Exostosin-1.
    IPR015338. HexNAc_Trfase_a.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR11062. PTHR11062. 1 hit.
    PTHR11062:SF45. PTHR11062:SF45. 1 hit.
    PfamiPF03016. Exostosin. 1 hit.
    PF09258. Glyco_transf_64. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5IGR7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAKKRYLIS LLTGAFLVLL IYLGGGGVPG PAAPGSRSRT HGYNRPDQPW    50
    PHFSDPLQHF SPWDHSDTED YNVHISPRQK RDVNSSVYKG KRCRMQSCFD 100
    FSLCQRNGFK VYVYPQQKGE KISESYQNIL STIEGSRFYT SDPGQACVFV 150
    LSLDTLDRDQ LSPQYVHNLK TKVQSLALWN NGRNHLIFNL YSGTWPDYTE 200
    DLGFDIGQAM LAKASISTEN FRPNFDISIP LFSKEHPRTG GDRGYLKYNT 250
    IPPFRKYMLV FKGKRYLTGI GSDTRNALYH VHNAEDVVLL TTCKHGKDWQ 300
    KHKDARCDKD NAEYDKYDYR EMLHNSTFCL VPRGRRLGSF RFLEALQAAC 350
    VPVMLSNGWE LPFSEVIDWN TAAVIGDERL LLQIPSTVRS IHQDKILALR 400
    QQTQFLWEAY FSSVEKIVLT TLEIIQDRVL QQSSRSSVMW NSHPGGLFSL 450
    PQYSSYLGDL PFFYAKLGIK PSPKFTAVIH AVTPLVSQSQ PILKLIVSVA 500
    RSQYCAQIIV LWNCDKPLPA KQRWPATAVP IIVIEGENKV MSSRFQPYES 550
    LISDAVLSLD EDTVLSTTEV DFAFTVWQSF PERIVGYPAR SHFWDNNKER 600
    WGYTSKWTND YSMVLTGAAI YHRYYHFLYT HFLPSSLKSM VDQLANCEDI 650
    LMNFLVSAVT KLPPVKVTQK KQYKETMMGQ SSRASRWADP DHFAQRQTCM 700
    NKFASWFGGM PLVHSQMRLD PVLFKDQVSI LRKKYREIER L 741
    Length:741
    Mass (Da):85,121
    Last modified:February 15, 2005 - v1
    Checksum:iC2793E62F5378393
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY734456 mRNA. Translation: AAW29034.1.
    UniGeneiDr.84943.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY734456 mRNA. Translation: AAW29034.1 .
    UniGenei Dr.84943.

    3D structure databases

    ProteinModelPortali Q5IGR7.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT47. Glycosyltransferase Family 47.
    GT64. Glycosyltransferase Family 64.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    ZFINi ZDB-GENE-050211-4. ext1b.

    Phylogenomic databases

    HOVERGENi HBG003459.
    PhylomeDBi Q5IGR7.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR004263. Exostosin.
    IPR027670. Exostosin-1.
    IPR015338. HexNAc_Trfase_a.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR11062. PTHR11062. 1 hit.
    PTHR11062:SF45. PTHR11062:SF45. 1 hit.
    Pfami PF03016. Exostosin. 1 hit.
    PF09258. Glyco_transf_64. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling."
      Siekmann A.F., Brand M.
      Dev. Dyn. 232:498-505(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiEXT1B_DANRE
    AccessioniPrimary (citable) accession number: Q5IGR7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3