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Q5IGR7

- EXT1B_DANRE

UniProt

Q5IGR7 - EXT1B_DANRE

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Protein

Exostosin-1b

Gene

ext1b

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

Manganese.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei513 – 5131SubstrateBy similarity
Metal bindingi562 – 5621Manganese; catalyticBy similarity
Binding sitei590 – 5901SubstrateBy similarity
Active sitei649 – 6491By similarity

GO - Molecular functioni

  1. glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Source: UniProtKB
  2. N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. heparan sulfate proteoglycan biosynthetic process Source: UniProtKB
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-1b (EC:2.4.1.224, EC:2.4.1.225)
Alternative name(s):
Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase 1b
Multiple exostoses protein 1 homolog b
Gene namesi
Name:ext1b
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Unplaced

Organism-specific databases

ZFINiZDB-GENE-050211-4. ext1b.

Subcellular locationi

GO - Cellular componenti

  1. integral component of endoplasmic reticulum membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 741741Exostosin-1bPRO_0000149660Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi325 – 3251N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi647 ↔ 699By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Developmental stagei

Expressed both maternally and zygotically. During gastrulation, expressed in the embryonic midline and in the involuting mesendoderm of the germ ring. During somatogenesis, expressed in the somitic mesoderm. At the 16-somite stage, expression is highest in the tailbud region, the eye and the dorsal somites. At 24 hours-post-fertilization (hpf), expressed in the neural tube, tailbud, posterior somites and forming fin bud. At 48 hpf, expressed in the fin buds and brain.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ5IGR7.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Topological domaini29 – 741713LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni539 – 5446Substrate bindingBy similarity
Regioni560 – 5623Substrate bindingBy similarity
Regioni645 – 6495Substrate bindingBy similarity
Regioni683 – 69614Substrate bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG003459.
InParanoidiQ5IGR7.
PhylomeDBiQ5IGR7.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004263. Exostosin.
IPR027670. Exostosin-1.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11062. PTHR11062. 1 hit.
PTHR11062:SF45. PTHR11062:SF45. 1 hit.
PfamiPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5IGR7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQAKKRYLIS LLTGAFLVLL IYLGGGGVPG PAAPGSRSRT HGYNRPDQPW
60 70 80 90 100
PHFSDPLQHF SPWDHSDTED YNVHISPRQK RDVNSSVYKG KRCRMQSCFD
110 120 130 140 150
FSLCQRNGFK VYVYPQQKGE KISESYQNIL STIEGSRFYT SDPGQACVFV
160 170 180 190 200
LSLDTLDRDQ LSPQYVHNLK TKVQSLALWN NGRNHLIFNL YSGTWPDYTE
210 220 230 240 250
DLGFDIGQAM LAKASISTEN FRPNFDISIP LFSKEHPRTG GDRGYLKYNT
260 270 280 290 300
IPPFRKYMLV FKGKRYLTGI GSDTRNALYH VHNAEDVVLL TTCKHGKDWQ
310 320 330 340 350
KHKDARCDKD NAEYDKYDYR EMLHNSTFCL VPRGRRLGSF RFLEALQAAC
360 370 380 390 400
VPVMLSNGWE LPFSEVIDWN TAAVIGDERL LLQIPSTVRS IHQDKILALR
410 420 430 440 450
QQTQFLWEAY FSSVEKIVLT TLEIIQDRVL QQSSRSSVMW NSHPGGLFSL
460 470 480 490 500
PQYSSYLGDL PFFYAKLGIK PSPKFTAVIH AVTPLVSQSQ PILKLIVSVA
510 520 530 540 550
RSQYCAQIIV LWNCDKPLPA KQRWPATAVP IIVIEGENKV MSSRFQPYES
560 570 580 590 600
LISDAVLSLD EDTVLSTTEV DFAFTVWQSF PERIVGYPAR SHFWDNNKER
610 620 630 640 650
WGYTSKWTND YSMVLTGAAI YHRYYHFLYT HFLPSSLKSM VDQLANCEDI
660 670 680 690 700
LMNFLVSAVT KLPPVKVTQK KQYKETMMGQ SSRASRWADP DHFAQRQTCM
710 720 730 740
NKFASWFGGM PLVHSQMRLD PVLFKDQVSI LRKKYREIER L
Length:741
Mass (Da):85,121
Last modified:February 15, 2005 - v1
Checksum:iC2793E62F5378393
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY734456 mRNA. Translation: AAW29034.1.
UniGeneiDr.84943.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY734456 mRNA. Translation: AAW29034.1 .
UniGenei Dr.84943.

3D structure databases

ProteinModelPortali Q5IGR7.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ZFINi ZDB-GENE-050211-4. ext1b.

Phylogenomic databases

HOVERGENi HBG003459.
InParanoidi Q5IGR7.
PhylomeDBi Q5IGR7.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR004263. Exostosin.
IPR027670. Exostosin-1.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR11062. PTHR11062. 1 hit.
PTHR11062:SF45. PTHR11062:SF45. 1 hit.
Pfami PF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling."
    Siekmann A.F., Brand M.
    Dev. Dyn. 232:498-505(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiEXT1B_DANRE
AccessioniPrimary (citable) accession number: Q5IGR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 15, 2005
Last modified: October 29, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3