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Protein

Exostosin-1b

Gene

ext1b

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei513SubstrateBy similarity1
Metal bindingi562Manganese; catalyticBy similarity1
Binding sitei590SubstrateBy similarity1
Active sitei649By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-1b (EC:2.4.1.224, EC:2.4.1.225)
Alternative name(s):
Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase 1b
Multiple exostoses protein 1 homolog b
Gene namesi
Name:ext1b
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-050211-4. ext1b.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 741LumenalSequence analysisAdd BLAST713

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001496601 – 741Exostosin-1bAdd BLAST741

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi84N-linked (GlcNAc...)Sequence analysis1
Glycosylationi325N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi647 ↔ 699By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5IGR7.
PRIDEiQ5IGR7.

Expressioni

Developmental stagei

Expressed both maternally and zygotically. During gastrulation, expressed in the embryonic midline and in the involuting mesendoderm of the germ ring. During somatogenesis, expressed in the somitic mesoderm. At the 16-somite stage, expression is highest in the tailbud region, the eye and the dorsal somites. At 24 hours-post-fertilization (hpf), expressed in the neural tube, tailbud, posterior somites and forming fin bud. At 48 hpf, expressed in the fin buds and brain.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000108198.

Structurei

3D structure databases

ProteinModelPortaliQ5IGR7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni539 – 544Substrate bindingBy similarity6
Regioni560 – 562Substrate bindingBy similarity3
Regioni645 – 649Substrate bindingBy similarity5
Regioni683 – 696Substrate bindingBy similarityAdd BLAST14

Sequence similaritiesi

Belongs to the glycosyltransferase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1021. Eukaryota.
ENOG410XTFH. LUCA.
HOVERGENiHBG003459.
InParanoidiQ5IGR7.
PhylomeDBiQ5IGR7.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004263. Exostosin.
IPR027670. Exostosin-1.
IPR015338. EXT_C.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11062:SF97. PTHR11062:SF97. 2 hits.
PfamiPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5IGR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAKKRYLIS LLTGAFLVLL IYLGGGGVPG PAAPGSRSRT HGYNRPDQPW
60 70 80 90 100
PHFSDPLQHF SPWDHSDTED YNVHISPRQK RDVNSSVYKG KRCRMQSCFD
110 120 130 140 150
FSLCQRNGFK VYVYPQQKGE KISESYQNIL STIEGSRFYT SDPGQACVFV
160 170 180 190 200
LSLDTLDRDQ LSPQYVHNLK TKVQSLALWN NGRNHLIFNL YSGTWPDYTE
210 220 230 240 250
DLGFDIGQAM LAKASISTEN FRPNFDISIP LFSKEHPRTG GDRGYLKYNT
260 270 280 290 300
IPPFRKYMLV FKGKRYLTGI GSDTRNALYH VHNAEDVVLL TTCKHGKDWQ
310 320 330 340 350
KHKDARCDKD NAEYDKYDYR EMLHNSTFCL VPRGRRLGSF RFLEALQAAC
360 370 380 390 400
VPVMLSNGWE LPFSEVIDWN TAAVIGDERL LLQIPSTVRS IHQDKILALR
410 420 430 440 450
QQTQFLWEAY FSSVEKIVLT TLEIIQDRVL QQSSRSSVMW NSHPGGLFSL
460 470 480 490 500
PQYSSYLGDL PFFYAKLGIK PSPKFTAVIH AVTPLVSQSQ PILKLIVSVA
510 520 530 540 550
RSQYCAQIIV LWNCDKPLPA KQRWPATAVP IIVIEGENKV MSSRFQPYES
560 570 580 590 600
LISDAVLSLD EDTVLSTTEV DFAFTVWQSF PERIVGYPAR SHFWDNNKER
610 620 630 640 650
WGYTSKWTND YSMVLTGAAI YHRYYHFLYT HFLPSSLKSM VDQLANCEDI
660 670 680 690 700
LMNFLVSAVT KLPPVKVTQK KQYKETMMGQ SSRASRWADP DHFAQRQTCM
710 720 730 740
NKFASWFGGM PLVHSQMRLD PVLFKDQVSI LRKKYREIER L
Length:741
Mass (Da):85,121
Last modified:February 15, 2005 - v1
Checksum:iC2793E62F5378393
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY734456 mRNA. Translation: AAW29034.1.
UniGeneiDr.84943.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY734456 mRNA. Translation: AAW29034.1.
UniGeneiDr.84943.

3D structure databases

ProteinModelPortaliQ5IGR7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000108198.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Proteomic databases

PaxDbiQ5IGR7.
PRIDEiQ5IGR7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ZFINiZDB-GENE-050211-4. ext1b.

Phylogenomic databases

eggNOGiKOG1021. Eukaryota.
ENOG410XTFH. LUCA.
HOVERGENiHBG003459.
InParanoidiQ5IGR7.
PhylomeDBiQ5IGR7.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiQ5IGR7.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004263. Exostosin.
IPR027670. Exostosin-1.
IPR015338. EXT_C.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11062:SF97. PTHR11062:SF97. 2 hits.
PfamiPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEXT1B_DANRE
AccessioniPrimary (citable) accession number: Q5IGR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 15, 2005
Last modified: October 5, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.