Reviewed, UniProtKB/Swiss-Prot Q5IGR7 (EXT1B_DANRE)

Names and origin

Protein names

Recommended name:
    Exostosin-1b
    EC=2.4.1.224
    EC=2.4.1.225
Alternative name(s):
    Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase 1b
    Multiple exostoses protein 1 homolog b

Gene names

Name:

ext1b

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	741 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosyltransferase required for the biosynthesis of heparan-sulfate By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan. UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Developmental stage	Expressed both maternally and zygotically. During gastrulation, expressed in the embryonic midline and in the involuting mesendoderm of the germ ring. During somatogenesis, expressed in the somitic mesoderm. At the 16-somite stage, expression is highest in the tailbud region, the eye and the dorsal somites. At 24 hours-post-fertilization (hpf), expressed in the neural tube, tailbud, posterior somites and forming fin bud. At 48 hpf, expressed in the fin buds and brain. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 47 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	heparan sulfate proteoglycan biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 741	741	Exostosin-1b		PRO_0000149660
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Signal-anchor for type II membrane protein Potential
	Topological domain	29 – 741	713	Lumenal Potential
Amino acid modifications
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	325	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5IGR7-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: C2793E62F5378393	FASTA	741	85,121
	10 20 30 40 50 60 MQAKKRYLIS LLTGAFLVLL IYLGGGGVPG PAAPGSRSRT HGYNRPDQPW PHFSDPLQHF 70 80 90 100 110 120 SPWDHSDTED YNVHISPRQK RDVNSSVYKG KRCRMQSCFD FSLCQRNGFK VYVYPQQKGE 130 140 150 160 170 180 KISESYQNIL STIEGSRFYT SDPGQACVFV LSLDTLDRDQ LSPQYVHNLK TKVQSLALWN 190 200 210 220 230 240 NGRNHLIFNL YSGTWPDYTE DLGFDIGQAM LAKASISTEN FRPNFDISIP LFSKEHPRTG 250 260 270 280 290 300 GDRGYLKYNT IPPFRKYMLV FKGKRYLTGI GSDTRNALYH VHNAEDVVLL TTCKHGKDWQ 310 320 330 340 350 360 KHKDARCDKD NAEYDKYDYR EMLHNSTFCL VPRGRRLGSF RFLEALQAAC VPVMLSNGWE 370 380 390 400 410 420 LPFSEVIDWN TAAVIGDERL LLQIPSTVRS IHQDKILALR QQTQFLWEAY FSSVEKIVLT 430 440 450 460 470 480 TLEIIQDRVL QQSSRSSVMW NSHPGGLFSL PQYSSYLGDL PFFYAKLGIK PSPKFTAVIH 490 500 510 520 530 540 AVTPLVSQSQ PILKLIVSVA RSQYCAQIIV LWNCDKPLPA KQRWPATAVP IIVIEGENKV 550 560 570 580 590 600 MSSRFQPYES LISDAVLSLD EDTVLSTTEV DFAFTVWQSF PERIVGYPAR SHFWDNNKER 610 620 630 640 650 660 WGYTSKWTND YSMVLTGAAI YHRYYHFLYT HFLPSSLKSM VDQLANCEDI LMNFLVSAVT 670 680 690 700 710 720 KLPPVKVTQK KQYKETMMGQ SSRASRWADP DHFAQRQTCM NKFASWFGGM PLVHSQMRLD 730 740 PVLFKDQVSI LRKKYREIER L

References

[1]

"Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling." Siekmann A.F., Brand M. Dev. Dyn. 232:498-505(2005) [PubMed: 15614771] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY734456 mRNA. Translation: AAW29034.1.
IPI	IPI00499249.
UniGene	Dr.124291 Dr.84943
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5IGR7.
Protein family/group databases
CAZy	GT47. Glycosyltransferase Family 47. GT64. Glycosyltransferase Family 64.
Organism-specific databases
ZFIN	ZDB-GENE-050211-4. ext1b.
Phylogenomic databases
HOVERGEN	Q5IGR7.
Enzyme and pathway databases
BRENDA	2.4.1.224. 96826. 2.4.1.225. 96826.
Family and domain databases
InterPro	IPR004263. Exostosin. IPR015338. HexNAc_Trfase_a. [Graphical view]
Pfam	PF03016. Exostosin. 1 hit. PF09258. Glyco_transf_64. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EXT1B_DANRE

Accession

Primary (citable) accession number: Q5IGR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	February 15, 2005
Last modified:	September 1, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project