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Q5IGR6

- EXT1C_DANRE

UniProt

Q5IGR6 - EXT1C_DANRE

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Protein

Exostosin-1c

Gene

ext1c

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei510 – 5101SubstrateBy similarity
Metal bindingi558 – 5581Manganese; catalyticBy similarity
Binding sitei586 – 5861SubstrateBy similarity
Active sitei645 – 6451By similarity

GO - Molecular functioni

  1. glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Source: UniProtKB
  2. N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. heparan sulfate proteoglycan biosynthetic process Source: UniProtKB
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-1c (EC:2.4.1.224, EC:2.4.1.225)
Alternative name(s):
Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase 1c
Multiple exostoses protein 1 homolog c
Gene namesi
Name:ext1c
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Unplaced

Organism-specific databases

ZFINiZDB-GENE-050211-5. ext1c.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini28 – 737710LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of endoplasmic reticulum membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 737737Exostosin-1cPRO_0000149661Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi194 – 1941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi643 ↔ 695By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ5IGR6.

Expressioni

Developmental stagei

Expressed zygotically. First detected at the beginning of somatogenesis. At the 16-somite stage, restricted to posterior adaxial cells and, in the anterior, to cells adjacent to the neural tube and to ventromedial reigons of the somites. At 24 hours-post-fertilization (hpf), expressed in the ventral rhombomeres, telencephalon and olfactory bulbs. At 48 hpf, expressed in the brain, retina and fin buds.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ5IGR6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni536 – 5405Substrate bindingBy similarity
Regioni556 – 5583Substrate bindingBy similarity
Regioni641 – 6455Substrate bindingBy similarity
Regioni679 – 69214Substrate bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG269038.
HOVERGENiHBG003459.
InParanoidiQ5IGR6.
PhylomeDBiQ5IGR6.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004263. Exostosin.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11062. PTHR11062. 1 hit.
PfamiPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5IGR6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQARKKYVLL GLCTCCWILL YYWAGLQERL LGLITHRRGE VPRPWPDWLD
60 70 80 90 100
RALLPNFAEQ LDLQNGGGPG DSPRQRKQAW SSIYKDSRCR MDTCFDFGRC
110 120 130 140 150
QTQSGFRVYI YPPEKGERVS EGYRKILTSV SESRYYTSDP REACLFVLGI
160 170 180 190 200
DTLDRDQLSQ QFVPNVDERI RGYPLWNDGR NHVIFNLYSG TWPNYTEDLG
210 220 230 240 250
FNVGQAILAK ASLNTEHFRP GFDISIPLFS KEHPQKGGKR GWLVRNSVPP
260 270 280 290 300
RRKYLLMFKG KRYLTGIGSD TRNALHHIHN GKDIVSLTTC RHGKDWEKHK
310 320 330 340 350
DARCDHDNQE YERFDYQELL HNSTFCLVPR GRRLGSFRFL ESLQAACIPV
360 370 380 390 400
LLSNGWELPF SDVIQWNQAV VEGDERLLLQ VPSTVRAVGI DRVLALRQQT
410 420 430 440 450
QTLWDAYFSS VDKIVLTTLE IIKDRVYSHI SRNKLMWNAL PGGLLVLPEF
460 470 480 490 500
STHLAHYPFY YLHLGISPGL EFTAVIHATS PLVSQSQPIM KLLQVVSKSK
510 520 530 540 550
YCSQIIILWN SEKSPPQRSK WPPMPVPLTV TDGRRKTSSR FLPHAAIETE
560 570 580 590 600
AVLSLDEDTV LLTSEINFAF HVWRSFPDRI VGYPPRSHFW DPVKKAWGYT
610 620 630 640 650
SKWTNEYSII LTGAAFYHRY YHHLFSHYLP SSLRALVDHS CNCEDILMNF
660 670 680 690 700
LVSSVAHLPP VKVAQRKQYK EMPSLQGTKM APWANPEHFT QRQECVNTFS
710 720 730
SWFGYMPLEH SQFRLDPVLF KDHVSVLRKR YKDLERV
Length:737
Mass (Da):85,389
Last modified:February 15, 2005 - v1
Checksum:i349EB644FB7AADB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY734457 mRNA. Translation: AAW29035.1.
UniGeneiDr.38024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY734457 mRNA. Translation: AAW29035.1 .
UniGenei Dr.38024.

3D structure databases

ProteinModelPortali Q5IGR6.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Proteomic databases

PRIDEi Q5IGR6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ZFINi ZDB-GENE-050211-5. ext1c.

Phylogenomic databases

eggNOGi NOG269038.
HOVERGENi HBG003459.
InParanoidi Q5IGR6.
PhylomeDBi Q5IGR6.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR004263. Exostosin.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR11062. PTHR11062. 1 hit.
Pfami PF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling."
    Siekmann A.F., Brand M.
    Dev. Dyn. 232:498-505(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiEXT1C_DANRE
AccessioniPrimary (citable) accession number: Q5IGR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 15, 2005
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3