Q5IGR6 (EXT1C_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exostosin-1c
EC=2.4.1.224
EC=2.4.1.225

Alternative name(s):
Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase 1c
Multiple exostoses protein 1 homolog c

Gene names

Name:

ext1c

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	737 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Glycosyltransferase required for the biosynthesis of heparan-sulfate By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan. UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Developmental stage	Expressed zygotically. First detected at the beginning of somatogenesis. At the 16-somite stage, restricted to posterior adaxial cells and, in the anterior, to cells adjacent to the neural tube and to ventromedial reigons of the somites. At 24 hours-post-fertilization (hpf), expressed in the ventral rhombomeres, telencephalon and olfactory bulbs. At 48 hpf, expressed in the brain, retina and fin buds. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 47 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heparan sulfate proteoglycan biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 737	737	Exostosin-1c		PRO_0000149661
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	28 – 737	710	Lumenal Potential
Amino acid modifications
	Glycosylation	194	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5IGR6 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 349EB644FB7AADB4	FASTA	737	85,389
	10 20 30 40 50 60 MQARKKYVLL GLCTCCWILL YYWAGLQERL LGLITHRRGE VPRPWPDWLD RALLPNFAEQ 70 80 90 100 110 120 LDLQNGGGPG DSPRQRKQAW SSIYKDSRCR MDTCFDFGRC QTQSGFRVYI YPPEKGERVS 130 140 150 160 170 180 EGYRKILTSV SESRYYTSDP REACLFVLGI DTLDRDQLSQ QFVPNVDERI RGYPLWNDGR 190 200 210 220 230 240 NHVIFNLYSG TWPNYTEDLG FNVGQAILAK ASLNTEHFRP GFDISIPLFS KEHPQKGGKR 250 260 270 280 290 300 GWLVRNSVPP RRKYLLMFKG KRYLTGIGSD TRNALHHIHN GKDIVSLTTC RHGKDWEKHK 310 320 330 340 350 360 DARCDHDNQE YERFDYQELL HNSTFCLVPR GRRLGSFRFL ESLQAACIPV LLSNGWELPF 370 380 390 400 410 420 SDVIQWNQAV VEGDERLLLQ VPSTVRAVGI DRVLALRQQT QTLWDAYFSS VDKIVLTTLE 430 440 450 460 470 480 IIKDRVYSHI SRNKLMWNAL PGGLLVLPEF STHLAHYPFY YLHLGISPGL EFTAVIHATS 490 500 510 520 530 540 PLVSQSQPIM KLLQVVSKSK YCSQIIILWN SEKSPPQRSK WPPMPVPLTV TDGRRKTSSR 550 560 570 580 590 600 FLPHAAIETE AVLSLDEDTV LLTSEINFAF HVWRSFPDRI VGYPPRSHFW DPVKKAWGYT 610 620 630 640 650 660 SKWTNEYSII LTGAAFYHRY YHHLFSHYLP SSLRALVDHS CNCEDILMNF LVSSVAHLPP 670 680 690 700 710 720 VKVAQRKQYK EMPSLQGTKM APWANPEHFT QRQECVNTFS SWFGYMPLEH SQFRLDPVLF 730 KDHVSVLRKR YKDLERV

References

[1]

"Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling." Siekmann A.F., Brand M. Dev. Dyn. 232:498-505(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY734457 mRNA. Translation: AAW29035.1.
IPI	IPI00554354.
UniGene	Dr.38024.
3D structure databases
ProteinModelPortal	Q5IGR6.
ModBase	Search...
Protein family/group databases
CAZy	GT47. Glycosyltransferase Family 47. GT64. Glycosyltransferase Family 64.
Proteomic databases
PRIDE	Q5IGR6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
ZFIN	ZDB-GENE-050211-5. ext1c.
Phylogenomic databases
eggNOG	NOG269038.
HOVERGEN	HBG003459.
OrthoDB	EOG47M1XC.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
InterPro	IPR004263. Exostosin. IPR015338. HexNAc_Trfase_a. [Graphical view]
Pfam	PF03016. Exostosin. 1 hit. PF09258. Glyco_transf_64. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EXT1C_DANRE

Accession

Primary (citable) accession number: Q5IGR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	February 15, 2005
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program