Q5IFP1 (3BHS_CANFA) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 56.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase Short name=3-beta-HSD | ||
| Gene names |
| ||
| Organism | Canis familiaris (Dog) (Canis lupus familiaris) | ||
| Taxonomic identifier | 9615 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis |
Protein attributes
| Sequence length | 373 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids By similarity. |
| Catalytic activity | A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion membrane; Single-pass membrane protein By similarity. |
| Sequence similarities | Belongs to the 3-beta-HSD family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Steroidogenesis |
| Cellular component | Endoplasmic reticulum Membrane Mitochondrion |
| Domain | Transmembrane Transmembrane helix |
| Ligand | NAD |
| Molecular function | Isomerase Oxidoreductase |
| Technical term | Complete proteome Multifunctional enzyme Reference proteome |
| Gene Ontology (GO) | |
| Biological process | steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW mitochondrial membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | 3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide bindingInferred from electronic annotation. Source: InterPro steroid delta-isomerase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 373 | 372 | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase | PRO_0000087772 | |||||
Regions | |||||||||
| Transmembrane | 288 – 308 | 21 | Helical; Potential | ||||||
Sites | |||||||||
| Active site | 155 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 159 | 1 | NAD By similarity | ||||||
Sequences
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References
| [1] | "Expression of 3-beta-hydroxysteroid dehydrogenase in canine corpus luteum during dioestrus." Kowalewski M.P., Taubert A., Hoffmann B. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Corpus luteum. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY739720 mRNA. Translation: AAW31741.1. |
| RefSeq | NP_001010954.1. NM_001010954.1. |
| UniGene | Cfa.13172. |
3D structure databases | |
| ProteinModelPortal | Q5IFP1. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q5IFP1. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 483146. |
| KEGG | cfa:483146. |
Organism-specific databases | |
| CTD | 3283. |
Phylogenomic databases | |
| eggNOG | maNOG10354. |
| GeneTree | ENSGT00550000074557. |
| HOVERGEN | HBG000014. |
| InParanoid | Q5IFP1. |
| OrthoDB | EOG4K3KWG. |
Family and domain databases | |
| InterPro | IPR002225. 3Beta_OHSteriod_DH/Estase. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K00070. |
| Pfam | PF01073. 3Beta_HSD. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | 3BHS_CANFA | ||||||||
| Accession | Primary (citable) accession number: Q5IFP1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with