Reviewed, UniProtKB/Swiss-Prot Q5IFP1 (3BHS_CANFA)

Names and origin

Protein names

Recommended name:
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
      Short name=3-beta-HSD
Including the following 2 domains:
    1- Recommended name:
            3-beta-hydroxy-Delta(5)-steroid dehydrogenase
              EC=1.1.1.145
        Alternative name(s):
            3-beta-hydroxy-5-ene steroid dehydrogenase
            Progesterone reductase
    2- Recommended name:
            Steroid Delta-isomerase
              EC=5.3.3.1
        Alternative name(s):
            Delta-5-3-ketosteroid isomerase

Gene names

Name:

HSD3B

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids By similarity.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD(+) = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane proteinBy similarity. Mitochondrion membrane; Single-pass membrane proteinBy similarity.
Sequence similarities	Belongs to the 3-beta-HSD family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 373	372	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase		PRO_0000087772
Regions
	Transmembrane	288 – 308	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5IFP1-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: EB12CB016D650A5B	FASTA	373	42,378
	10 20 30 40 50 60 MAGWSCLVTG AGGFLGQRIV HLLAEEKELQ EIRALDKAFR PELLEEFSKL QSKTKLTMVE 70 80 90 100 110 120 GDILDEQCLK RACQGTSVVI HTASVIDVMN VIHRETIMNV NLKGTQLLLE ACAQASVPIF 130 140 150 160 170 180 IYTSTIEVAG PNSYRDIIQN AHEEEHLEST WSAPYPYSKK LAEKAVLAAN GWALKNGGTL 190 200 210 220 230 240 HTCALRPMYI YGEGSIFLYN YIYKALRNNG ILTHHSKFSI VNPVYVGNVA WAHILALRAL 250 260 270 280 290 300 QDPKKAPSVQ GQFYYISDDT PHQSYDDLNY NLSKEWGFSL DSRMSLPISL EYWLAFLLEI 310 320 330 340 350 360 VSFLLSPIYK YQPPFNRHMV TLSNSIFTFS YKKAQRDLGY KPLFSWEEAK QKTTEWIGSL 370 VKQHKETLKT KTH

References

[1]	"Expression of 3-beta-hydroxysteroid dehydrogenase in canine corpus luteum during dioestrus." Kowalewski M.P., Taubert A., Hoffmann B. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Corpus luteum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY739720 mRNA. Translation: AAW31741.1.
RefSeq	NP_001010954.1.
UniGene	Cfa.13172
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSCAFG00000010039. Canis familiaris. [Contig view]
GeneID	483146.
KEGG	cfa:483146.
Phylogenomic databases
HOVERGEN	Q5IFP1.
Family and domain databases
InterPro	IPR002225. 3Beta_OHSteriod_DHase/Estase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01073. 3Beta_HSD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

3BHS_CANFA

Accession

Primary (citable) accession number: Q5IFP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 31 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)