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Protein

Growth factor receptor-bound protein 14

Gene

GRB14

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Growth factor receptor-bound protein 14
Alternative name(s):
GRB14 adapter protein
Gene namesi
Name:GRB14
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm By similarity
  • Endosome membrane By similarity; Peripheral membrane protein By similarity

  • Note: Upon insulin stimulation, translocates to the plasma membrane.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 540539Growth factor receptor-bound protein 14PRO_0000253502Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity
Modified residuei372 – 3721PhosphoserineBy similarity
Modified residuei375 – 3751PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5ICW4.
PRIDEiQ5ICW4.

Interactioni

Subunit structurei

Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor, through the SH2 domain. Interacts with GRB14 (via BPS domain); this interaction protects the tyrosines in the activation loop on INSR from dephosphorylation (By similarity). Binds to the ankyrin repeat region of TNKS2 via its N-terminus. Interacts with activated NRAS. Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CNGA1P299734EBI-7639273,EBI-8417095From a different organism.
CNGB1Q140282EBI-7639273,EBI-7959609From a different organism.

Protein-protein interaction databases

IntActiQ5ICW4. 2 interactions.
MINTiMINT-1348029.
STRINGi9913.ENSBTAP00000025686.

Structurei

3D structure databases

ProteinModelPortaliQ5ICW4.
SMRiQ5ICW4. Positions 373-409, 433-537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini106 – 19287Ras-associatingPROSITE-ProRule annotationAdd
BLAST
Domaini234 – 342109PHPROSITE-ProRule annotationAdd
BLAST
Domaini439 – 53597SH2PROSITE-ProRule annotationAdd
BLAST

Domaini

The PH domain binds relatively non-specifically and with low affinity to several phosphoinositides, the best binder being PI(3,4,5)P3.By similarity

Sequence similaritiesi

Belongs to the GRB7/10/14 family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiENOG410KD2U. Eukaryota.
ENOG410ZN6J. LUCA.
HOGENOMiHOG000231904.
HOVERGENiHBG000468.
InParanoidiQ5ICW4.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR015042. BPS-dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR000980. SH2.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08947. BPS. 1 hit.
PF00169. PH. 1 hit.
PF00788. RA. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50200. RA. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5ICW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSLQDGQS AAGRAAARDS PLAAQVCGAA QGRGDARDLA PAPWLHARAL
60 70 80 90 100
LPPPDATRGC AADRRKKKDL DVLEMPSIPN PFPELCCSPF TSVLSAGLFP
110 120 130 140 150
KANSRKKQVI KVYSEDETSR ALEVPSDITA RDVCQLLILK NHYIDDHSWT
160 170 180 190 200
LFEHLPHVGL ERTIEDHELV IEVLSNWGME EENKLYFRKN YAKYEFFKNP
210 220 230 240 250
MYFFPEHMVS FATETNGEIS PTQILQMFLS SSTYPEIHGF LHAKEQGKKS
260 270 280 290 300
WKKIYFLLRR SGLYFSTKGT SKEPRHLQFF SEFGNSDIYV SLAGKKKHGA
310 320 330 340 350
PTNYGFCFKP NKAGGPRDLK MLCAEEEQSR TCWVTAIRLL KYGMQLYQNY
360 370 380 390 400
MHPYQGRSGY SSQSISPMRS ISENSRVAMD FSGQKTRVIE NPTEALSVAV
410 420 430 440 450
EEGLAWRKKG CLRLGSHGSP TASSQSSATS MAIHRSQPWF HHKMSREEAQ
460 470 480 490 500
RLIIQQGLVD GVFLVRDSQS NPKTFVLSMS HGQKIKHFQI IPVEDDGEMF
510 520 530 540
HTLDDGHTRF TDLIQLVEFY QLNKGVLPCK LKHYCARIAL
Length:540
Mass (Da):61,040
Last modified:February 15, 2005 - v1
Checksum:i886BE74B11F1D062
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY772475 mRNA. Translation: AAW47246.1.
RefSeqiNP_001011681.2. NM_001011681.3.
UniGeneiBt.36371.

Genome annotation databases

GeneIDi497029.
KEGGibta:497029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY772475 mRNA. Translation: AAW47246.1.
RefSeqiNP_001011681.2. NM_001011681.3.
UniGeneiBt.36371.

3D structure databases

ProteinModelPortaliQ5ICW4.
SMRiQ5ICW4. Positions 373-409, 433-537.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5ICW4. 2 interactions.
MINTiMINT-1348029.
STRINGi9913.ENSBTAP00000025686.

Proteomic databases

PaxDbiQ5ICW4.
PRIDEiQ5ICW4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi497029.
KEGGibta:497029.

Organism-specific databases

CTDi2888.

Phylogenomic databases

eggNOGiENOG410KD2U. Eukaryota.
ENOG410ZN6J. LUCA.
HOGENOMiHOG000231904.
HOVERGENiHBG000468.
InParanoidiQ5ICW4.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR015042. BPS-dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR000980. SH2.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08947. BPS. 1 hit.
PF00169. PH. 1 hit.
PF00788. RA. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50200. RA. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRB14_BOVIN
AccessioniPrimary (citable) accession number: Q5ICW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: February 15, 2005
Last modified: June 8, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.