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Q5IBH7 (SPDYA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Speedy protein A
Alternative name(s):
Protein expressed in male leptotene and zygotene spermatocytes 465
Short name=MLZ-465
Rapid inducer of G2/M progression in oocytes A
Short name=RINGO A
Short name=mSpy/Ringo A
Speedy-1
Short name=Spy1
Gene names
Name:Spdya
Synonyms:Spdy1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP1. Can activate CDK2 without promoting CDK2 phosphorylation. Mediates cell survival during the DNA damage process through activation of CDK2. Ref.1 UniProtKB Q5MJ70

Subunit structure

Interacts with CDK1, CDK2 and CDKN1B/KIP1. Found in a complex with both CDK2 and CDKN1B/KIP1. Ref.1

Subcellular location

Nucleus Ref.5.

Tissue specificity

Expressed at a high level in testis. Also expressed in the adult ovary and in immature oocytes. Ref.1 Ref.4 Ref.5

Domain

The C-terminus is required for CDK2-activation, but not CDK2-binding. Ref.1

Sequence similarities

Belongs to the Speedy/Ringo family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 Ref.1 (identifier: Q5IBH7-2)

Also known as: A2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 Ref.1 (identifier: Q5IBH7-1)

Also known as: A1;

The sequence of this isoform differs from the canonical sequence as follows:
     281-283: VAN → GMA
     284-310: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 310310Speedy protein A
PRO_0000234113

Regions

Region67 – 199133Speedy/Ringo box; Required for CDK-binding Ref.1

Natural variations

Alternative sequence281 – 2833VAN → GMA in isoform 1. Ref.1
VSP_052029
Alternative sequence284 – 31027Missing in isoform 1. Ref.1
VSP_052030

Experimental info

Mutagenesis891D → A: Abolishes interaction with CDK2. Ref.1
Mutagenesis1021M → A: Abolishes interaction with CDK2. Ref.1
Mutagenesis1061Y → A: Abolishes interaction with CDK2. Ref.1
Mutagenesis1071F → A: Abolishes interaction with CDK2. Ref.1
Mutagenesis133 – 1353EED → AAA: No effect on CDK2-binding. Ref.1
Sequence conflict2581D → G in AAW32476. Ref.1
Sequence conflict2581D → G in AAW32477. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 (A2) [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 10F372690FF03826

FASTA31036,115
        10         20         30         40         50         60 
MRHNQMYCET PPTVTIHVKS GSNRSHQTRK PISLKRPILK DSWEASENNA QNNKSKRPRG 

        70         80         90        100        110        120 
PCLIIQRQEM TAFFKLFDDD LIQDFLWMDC CCKIADKYLL AMTFVYFKRA KFTINEHTRI 

       130        140        150        160        170        180 
NFFIALYLAN TVEEDEEEAK YEIFPWALGK NWRKLFPNFL KLRDQLWDRI DYRAIVSRRC 

       190        200        210        220        230        240 
CEEVMAIAPT HYIWQRERSV HHSGAVRNYN RDEVHLPRGP SATPVDCSLC GKKGRYVRLG 

       250        260        270        280        290        300 
LSSSSSSSSD TGELMEKDSQ ELHSAFSVDT AGDPPHTYSQ VANDHQSNKE NETNFVKKNK 

       310 
SVEWFAESEE 

« Hide

Isoform 1 (A1) [UniParc].

Checksum: 2240421315D3FD96
Show »

FASTA28332,853

References

« Hide 'large scale' references
[1]"Identification and comparative analysis of multiple mammalian Speedy/Ringo proteins."
Cheng A., Xiong W., Ferrell J.E. Jr., Solomon M.J.
Cell Cycle 4:155-165(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, INTERACTION WITH CDK1 AND CDK2, TISSUE SPECIFICITY, DOMAIN, MUTAGENESIS OF ASP-89; MET-102; TYR-106; PHE-107 AND 133-GLU--ASP-135.
Strain: BALB/c.
Tissue: Testis.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Testis.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"RINGO efficiently triggers meiosis resumption in mouse oocytes and induces cell cycle arrest in embryos."
Terret M.E., Ferby I., Nebreda A.R., Verlhac M.H.
Biol. Cell 93:89-97(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Screening of genes involved in chromosome segregation during meiosis I: toward the identification of genes responsible for infertility in humans."
Kogo H., Kowa-Sugiyama H., Yamada K., Bolor H., Tsutsumi M., Ohye T., Inagaki H., Taniguchi M., Toda T., Kurahashi H.
J. Hum. Genet. 55:293-299(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY820306 mRNA. Translation: AAW32476.1.
AY820307 mRNA. Translation: AAW32477.1.
AK077138 mRNA. Translation: BAC36637.1.
CT010429 Genomic DNA. Translation: CAX16044.1.
CCDSCCDS50178.1. [Q5IBH7-2]
CCDS50179.1. [Q5IBH7-1]
RefSeqNP_001136103.1. NM_001142631.1. [Q5IBH7-1]
NP_083530.1. NM_029254.1. [Q5IBH7-2]
XP_006524970.1. XM_006524907.1. [Q5IBH7-2]
XP_006524971.1. XM_006524908.1. [Q5IBH7-2]
UniGeneMm.477599.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000118994.

PTM databases

PhosphoSiteQ5IBH7.

Proteomic databases

PRIDEQ5IBH7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000124001; ENSMUSP00000118426; ENSMUSG00000052525. [Q5IBH7-1]
ENSMUST00000144142; ENSMUSP00000118994; ENSMUSG00000052525. [Q5IBH7-2]
ENSMUST00000167641; ENSMUSP00000125912; ENSMUSG00000052525. [Q5IBH7-2]
GeneID70891.
KEGGmmu:70891.
UCSCuc008dmo.2. mouse. [Q5IBH7-2]

Organism-specific databases

CTD245711.
MGIMGI:1918141. Spdya.

Phylogenomic databases

eggNOGNOG42793.
GeneTreeENSGT00420000029723.
HOGENOMHOG000060281.
HOVERGENHBG086798.
InParanoidQ5IBH7.
KOK08694.
OMAKHSQESH.
OrthoDBEOG7PS1FZ.
TreeFamTF329827.

Gene expression databases

BgeeQ5IBH7.
CleanExMM_SPDYA.
GenevestigatorQ5IBH7.

Family and domain databases

InterProIPR020984. Cell_cycle_regulatory_Spy1.
[Graphical view]
PfamPF11357. Spy1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio332520.
PROQ5IBH7.
SOURCESearch...

Entry information

Entry nameSPDYA_MOUSE
AccessionPrimary (citable) accession number: Q5IBH7
Secondary accession number(s): B8JJC0, Q5IBH8, Q8C5T5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot