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Q5IBH7

- SPDYA_MOUSE

UniProt

Q5IBH7 - SPDYA_MOUSE

Protein

Speedy protein A

Gene

Spdya

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP1. Can activate CDK2 without promoting CDK2 phosphorylation. Mediates cell survival during the DNA damage process through activation of CDK2.1 Publication

    GO - Molecular functioni

    1. protein kinase binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: UniProtKB-KW
    2. G1/S transition of mitotic cell cycle Source: UniProtKB
    3. male meiosis Source: UniProtKB
    4. multicellular organismal development Source: UniProtKB-KW
    5. positive regulation of cell proliferation Source: Ensembl
    6. positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Cell cycle, DNA damage

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Speedy protein A
    Alternative name(s):
    Protein expressed in male leptotene and zygotene spermatocytes 465
    Short name:
    MLZ-465
    Rapid inducer of G2/M progression in oocytes A
    Short name:
    RINGO A
    Short name:
    mSpy/Ringo A
    Speedy-1
    Short name:
    Spy1
    Gene namesi
    Name:SpdyaImported
    Synonyms:Spdy1Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1918141. Spdya.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi89 – 891D → A: Abolishes interaction with CDK2. 1 Publication
    Mutagenesisi102 – 1021M → A: Abolishes interaction with CDK2. 1 Publication
    Mutagenesisi106 – 1061Y → A: Abolishes interaction with CDK2. 1 Publication
    Mutagenesisi107 – 1071F → A: Abolishes interaction with CDK2. 1 Publication
    Mutagenesisi133 – 1353EED → AAA: No effect on CDK2-binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 310310Speedy protein APRO_0000234113Add
    BLAST

    Proteomic databases

    PRIDEiQ5IBH7.

    PTM databases

    PhosphoSiteiQ5IBH7.

    Expressioni

    Tissue specificityi

    Expressed at a high level in testis. Also expressed in the adult ovary and in immature oocytes.3 Publications

    Gene expression databases

    BgeeiQ5IBH7.
    CleanExiMM_SPDYA.
    GenevestigatoriQ5IBH7.

    Interactioni

    Subunit structurei

    Interacts with CDK1, CDK2 and CDKN1B/KIP1. Found in a complex with both CDK2 and CDKN1B/KIP1.1 Publication

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000118994.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni67 – 199133Speedy/Ringo box; Required for CDK-binding1 PublicationAdd
    BLAST

    Domaini

    The C-terminus is required for CDK2-activation, but not CDK2-binding.1 Publication

    Sequence similaritiesi

    Belongs to the Speedy/Ringo family.Curated

    Phylogenomic databases

    eggNOGiNOG42793.
    GeneTreeiENSGT00420000029723.
    HOGENOMiHOG000060281.
    HOVERGENiHBG086798.
    InParanoidiQ5IBH7.
    KOiK08694.
    OMAiKHSQESH.
    OrthoDBiEOG7PS1FZ.
    TreeFamiTF329827.

    Family and domain databases

    InterProiIPR020984. Cell_cycle_regulatory_Spy1.
    [Graphical view]
    PfamiPF11357. Spy1. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 21 Publication (identifier: Q5IBH7-2) [UniParc]FASTAAdd to Basket

    Also known as: A21 Publication

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRHNQMYCET PPTVTIHVKS GSNRSHQTRK PISLKRPILK DSWEASENNA    50
    QNNKSKRPRG PCLIIQRQEM TAFFKLFDDD LIQDFLWMDC CCKIADKYLL 100
    AMTFVYFKRA KFTINEHTRI NFFIALYLAN TVEEDEEEAK YEIFPWALGK 150
    NWRKLFPNFL KLRDQLWDRI DYRAIVSRRC CEEVMAIAPT HYIWQRERSV 200
    HHSGAVRNYN RDEVHLPRGP SATPVDCSLC GKKGRYVRLG LSSSSSSSSD 250
    TGELMEKDSQ ELHSAFSVDT AGDPPHTYSQ VANDHQSNKE NETNFVKKNK 300
    SVEWFAESEE 310
    Length:310
    Mass (Da):36,115
    Last modified:July 27, 2011 - v2
    Checksum:i10F372690FF03826
    GO
    Isoform 11 Publication (identifier: Q5IBH7-1) [UniParc]FASTAAdd to Basket

    Also known as: A11 Publication

    The sequence of this isoform differs from the canonical sequence as follows:
         281-283: VAN → GMA
         284-310: Missing.

    Show »
    Length:283
    Mass (Da):32,853
    Checksum:i2240421315D3FD96
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti258 – 2581D → G in AAW32476. (PubMed:15611625)Curated
    Sequence conflicti258 – 2581D → G in AAW32477. (PubMed:15611625)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei281 – 2833VAN → GMA in isoform 1. 2 PublicationsVSP_052029
    Alternative sequencei284 – 31027Missing in isoform 1. 2 PublicationsVSP_052030Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY820306 mRNA. Translation: AAW32476.1.
    AY820307 mRNA. Translation: AAW32477.1.
    AK077138 mRNA. Translation: BAC36637.1.
    CT010429 Genomic DNA. Translation: CAX16044.1.
    CCDSiCCDS50178.1. [Q5IBH7-2]
    CCDS50179.1. [Q5IBH7-1]
    RefSeqiNP_001136103.1. NM_001142631.1. [Q5IBH7-1]
    NP_083530.1. NM_029254.1. [Q5IBH7-2]
    XP_006524970.1. XM_006524907.1. [Q5IBH7-2]
    XP_006524971.1. XM_006524908.1. [Q5IBH7-2]
    UniGeneiMm.477599.

    Genome annotation databases

    EnsembliENSMUST00000124001; ENSMUSP00000118426; ENSMUSG00000052525. [Q5IBH7-1]
    ENSMUST00000144142; ENSMUSP00000118994; ENSMUSG00000052525. [Q5IBH7-2]
    ENSMUST00000167641; ENSMUSP00000125912; ENSMUSG00000052525. [Q5IBH7-2]
    GeneIDi70891.
    KEGGimmu:70891.
    UCSCiuc008dmo.2. mouse. [Q5IBH7-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY820306 mRNA. Translation: AAW32476.1 .
    AY820307 mRNA. Translation: AAW32477.1 .
    AK077138 mRNA. Translation: BAC36637.1 .
    CT010429 Genomic DNA. Translation: CAX16044.1 .
    CCDSi CCDS50178.1. [Q5IBH7-2 ]
    CCDS50179.1. [Q5IBH7-1 ]
    RefSeqi NP_001136103.1. NM_001142631.1. [Q5IBH7-1 ]
    NP_083530.1. NM_029254.1. [Q5IBH7-2 ]
    XP_006524970.1. XM_006524907.1. [Q5IBH7-2 ]
    XP_006524971.1. XM_006524908.1. [Q5IBH7-2 ]
    UniGenei Mm.477599.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000118994.

    PTM databases

    PhosphoSitei Q5IBH7.

    Proteomic databases

    PRIDEi Q5IBH7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000124001 ; ENSMUSP00000118426 ; ENSMUSG00000052525 . [Q5IBH7-1 ]
    ENSMUST00000144142 ; ENSMUSP00000118994 ; ENSMUSG00000052525 . [Q5IBH7-2 ]
    ENSMUST00000167641 ; ENSMUSP00000125912 ; ENSMUSG00000052525 . [Q5IBH7-2 ]
    GeneIDi 70891.
    KEGGi mmu:70891.
    UCSCi uc008dmo.2. mouse. [Q5IBH7-2 ]

    Organism-specific databases

    CTDi 245711.
    MGIi MGI:1918141. Spdya.

    Phylogenomic databases

    eggNOGi NOG42793.
    GeneTreei ENSGT00420000029723.
    HOGENOMi HOG000060281.
    HOVERGENi HBG086798.
    InParanoidi Q5IBH7.
    KOi K08694.
    OMAi KHSQESH.
    OrthoDBi EOG7PS1FZ.
    TreeFami TF329827.

    Miscellaneous databases

    NextBioi 332520.
    PROi Q5IBH7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q5IBH7.
    CleanExi MM_SPDYA.
    Genevestigatori Q5IBH7.

    Family and domain databases

    InterProi IPR020984. Cell_cycle_regulatory_Spy1.
    [Graphical view ]
    Pfami PF11357. Spy1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and comparative analysis of multiple mammalian Speedy/Ringo proteins."
      Cheng A., Xiong W., Ferrell J.E. Jr., Solomon M.J.
      Cell Cycle 4:155-165(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, INTERACTION WITH CDK1 AND CDK2, TISSUE SPECIFICITY, DOMAIN, MUTAGENESIS OF ASP-89; MET-102; TYR-106; PHE-107 AND 133-GLU--ASP-135.
      Strain: BALB/cImported.
      Tissue: TestisImported.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6JImported.
      Tissue: TestisImported.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "RINGO efficiently triggers meiosis resumption in mouse oocytes and induces cell cycle arrest in embryos."
      Terret M.E., Ferby I., Nebreda A.R., Verlhac M.H.
      Biol. Cell 93:89-97(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    5. "Screening of genes involved in chromosome segregation during meiosis I: toward the identification of genes responsible for infertility in humans."
      Kogo H., Kowa-Sugiyama H., Yamada K., Bolor H., Tsutsumi M., Ohye T., Inagaki H., Taniguchi M., Toda T., Kurahashi H.
      J. Hum. Genet. 55:293-299(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiSPDYA_MOUSE
    AccessioniPrimary (citable) accession number: Q5IBH7
    Secondary accession number(s): B8JJC0, Q5IBH8, Q8C5T5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2006
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 69 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3