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Protein

Speedy protein A

Gene

Spdya

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP1. Can activate CDK2 without promoting CDK2 phosphorylation. Mediates cell survival during the DNA damage process through activation of CDK2.1 Publication

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell cycle, DNA damage

Names & Taxonomyi

Protein namesi
Recommended name:
Speedy protein A
Alternative name(s):
Protein expressed in male leptotene and zygotene spermatocytes 465
Short name:
MLZ-465
Rapid inducer of G2/M progression in oocytes A
Short name:
RINGO A
Short name:
mSpy/Ringo A
Speedy-1
Short name:
Spy1
Gene namesi
Name:SpdyaImported
Synonyms:Spdy1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1918141. Spdya.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi89 – 891D → A: Abolishes interaction with CDK2. 1 Publication
Mutagenesisi102 – 1021M → A: Abolishes interaction with CDK2. 1 Publication
Mutagenesisi106 – 1061Y → A: Abolishes interaction with CDK2. 1 Publication
Mutagenesisi107 – 1071F → A: Abolishes interaction with CDK2. 1 Publication
Mutagenesisi133 – 1353EED → AAA: No effect on CDK2-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Speedy protein APRO_0000234113Add
BLAST

Proteomic databases

PRIDEiQ5IBH7.

PTM databases

PhosphoSiteiQ5IBH7.

Expressioni

Tissue specificityi

Expressed at a high level in testis. Also expressed in the adult ovary and in immature oocytes.3 Publications

Gene expression databases

BgeeiQ5IBH7.
CleanExiMM_SPDYA.
ExpressionAtlasiQ5IBH7. baseline and differential.
GenevisibleiQ5IBH7. MM.

Interactioni

Subunit structurei

Interacts with CDK1, CDK2 and CDKN1B/KIP1. Found in a complex with both CDK2 and CDKN1B/KIP1.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000118994.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 199133Speedy/Ringo box; Required for CDK-binding1 PublicationAdd
BLAST

Domaini

The C-terminus is required for CDK2-activation, but not CDK2-binding.1 Publication

Sequence similaritiesi

Belongs to the Speedy/Ringo family.Curated

Phylogenomic databases

eggNOGiNOG42793.
GeneTreeiENSGT00420000029723.
HOGENOMiHOG000060281.
HOVERGENiHBG086798.
InParanoidiQ5IBH7.
KOiK08694.
OMAiEVMEKHS.
OrthoDBiEOG7PS1FZ.
TreeFamiTF329827.

Family and domain databases

InterProiIPR020984. Speedy.
[Graphical view]
PfamiPF11357. Spy1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 21 Publication (identifier: Q5IBH7-2) [UniParc]FASTAAdd to basket

Also known as: A21 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRHNQMYCET PPTVTIHVKS GSNRSHQTRK PISLKRPILK DSWEASENNA
60 70 80 90 100
QNNKSKRPRG PCLIIQRQEM TAFFKLFDDD LIQDFLWMDC CCKIADKYLL
110 120 130 140 150
AMTFVYFKRA KFTINEHTRI NFFIALYLAN TVEEDEEEAK YEIFPWALGK
160 170 180 190 200
NWRKLFPNFL KLRDQLWDRI DYRAIVSRRC CEEVMAIAPT HYIWQRERSV
210 220 230 240 250
HHSGAVRNYN RDEVHLPRGP SATPVDCSLC GKKGRYVRLG LSSSSSSSSD
260 270 280 290 300
TGELMEKDSQ ELHSAFSVDT AGDPPHTYSQ VANDHQSNKE NETNFVKKNK
310
SVEWFAESEE
Length:310
Mass (Da):36,115
Last modified:July 27, 2011 - v2
Checksum:i10F372690FF03826
GO
Isoform 11 Publication (identifier: Q5IBH7-1) [UniParc]FASTAAdd to basket

Also known as: A11 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     281-283: VAN → GMA
     284-310: Missing.

Show »
Length:283
Mass (Da):32,853
Checksum:i2240421315D3FD96
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti258 – 2581D → G in AAW32476 (PubMed:15611625).Curated
Sequence conflicti258 – 2581D → G in AAW32477 (PubMed:15611625).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei281 – 2833VAN → GMA in isoform 1. 2 PublicationsVSP_052029
Alternative sequencei284 – 31027Missing in isoform 1. 2 PublicationsVSP_052030Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY820306 mRNA. Translation: AAW32476.1.
AY820307 mRNA. Translation: AAW32477.1.
AK077138 mRNA. Translation: BAC36637.1.
CT010429 Genomic DNA. Translation: CAX16044.1.
CCDSiCCDS50178.1. [Q5IBH7-2]
CCDS50179.1. [Q5IBH7-1]
RefSeqiNP_001136103.1. NM_001142631.1. [Q5IBH7-1]
NP_083530.1. NM_029254.1. [Q5IBH7-2]
XP_006524970.1. XM_006524907.1. [Q5IBH7-2]
XP_006524971.1. XM_006524908.1. [Q5IBH7-2]
UniGeneiMm.477599.

Genome annotation databases

EnsembliENSMUST00000124001; ENSMUSP00000118426; ENSMUSG00000052525. [Q5IBH7-1]
ENSMUST00000144142; ENSMUSP00000118994; ENSMUSG00000052525. [Q5IBH7-2]
ENSMUST00000167641; ENSMUSP00000125912; ENSMUSG00000052525. [Q5IBH7-2]
GeneIDi70891.
KEGGimmu:70891.
UCSCiuc008dmo.2. mouse. [Q5IBH7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY820306 mRNA. Translation: AAW32476.1.
AY820307 mRNA. Translation: AAW32477.1.
AK077138 mRNA. Translation: BAC36637.1.
CT010429 Genomic DNA. Translation: CAX16044.1.
CCDSiCCDS50178.1. [Q5IBH7-2]
CCDS50179.1. [Q5IBH7-1]
RefSeqiNP_001136103.1. NM_001142631.1. [Q5IBH7-1]
NP_083530.1. NM_029254.1. [Q5IBH7-2]
XP_006524970.1. XM_006524907.1. [Q5IBH7-2]
XP_006524971.1. XM_006524908.1. [Q5IBH7-2]
UniGeneiMm.477599.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000118994.

PTM databases

PhosphoSiteiQ5IBH7.

Proteomic databases

PRIDEiQ5IBH7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000124001; ENSMUSP00000118426; ENSMUSG00000052525. [Q5IBH7-1]
ENSMUST00000144142; ENSMUSP00000118994; ENSMUSG00000052525. [Q5IBH7-2]
ENSMUST00000167641; ENSMUSP00000125912; ENSMUSG00000052525. [Q5IBH7-2]
GeneIDi70891.
KEGGimmu:70891.
UCSCiuc008dmo.2. mouse. [Q5IBH7-2]

Organism-specific databases

CTDi245711.
MGIiMGI:1918141. Spdya.

Phylogenomic databases

eggNOGiNOG42793.
GeneTreeiENSGT00420000029723.
HOGENOMiHOG000060281.
HOVERGENiHBG086798.
InParanoidiQ5IBH7.
KOiK08694.
OMAiEVMEKHS.
OrthoDBiEOG7PS1FZ.
TreeFamiTF329827.

Miscellaneous databases

NextBioi332520.
PROiQ5IBH7.
SOURCEiSearch...

Gene expression databases

BgeeiQ5IBH7.
CleanExiMM_SPDYA.
ExpressionAtlasiQ5IBH7. baseline and differential.
GenevisibleiQ5IBH7. MM.

Family and domain databases

InterProiIPR020984. Speedy.
[Graphical view]
PfamiPF11357. Spy1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and comparative analysis of multiple mammalian Speedy/Ringo proteins."
    Cheng A., Xiong W., Ferrell J.E. Jr., Solomon M.J.
    Cell Cycle 4:155-165(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, INTERACTION WITH CDK1 AND CDK2, TISSUE SPECIFICITY, DOMAIN, MUTAGENESIS OF ASP-89; MET-102; TYR-106; PHE-107 AND 133-GLU--ASP-135.
    Strain: BALB/cImported.
    Tissue: TestisImported.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6JImported.
    Tissue: TestisImported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "RINGO efficiently triggers meiosis resumption in mouse oocytes and induces cell cycle arrest in embryos."
    Terret M.E., Ferby I., Nebreda A.R., Verlhac M.H.
    Biol. Cell 93:89-97(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Screening of genes involved in chromosome segregation during meiosis I: toward the identification of genes responsible for infertility in humans."
    Kogo H., Kowa-Sugiyama H., Yamada K., Bolor H., Tsutsumi M., Ohye T., Inagaki H., Taniguchi M., Toda T., Kurahashi H.
    J. Hum. Genet. 55:293-299(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSPDYA_MOUSE
AccessioniPrimary (citable) accession number: Q5IBH7
Secondary accession number(s): B8JJC0, Q5IBH8, Q8C5T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.