Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase

Gene

ALG10B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Putative alpha-1,2-glucosyltransferase, which adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc2-PP-Dol (By similarity). When coupled to KCNH2 may reduce KCNH2 sensitivity to classic proarrhythmic drug blockade, possibly by mediating glycosylation of KCNH2 (PubMed:14525949). Has a role in maintenance of cochlear outer hair cell function (By similarity).By similarity1 Publication

Caution

PubMed:15280551 reports a Val-447 variant, however there is evidence that the right variant is Val-446.Curated

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->2)-D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • dolichol-linked oligosaccharide biosynthetic process Source: InterPro
  • positive regulation of inward rectifier potassium channel activity Source: UniProtKB
  • positive regulation of protein glycosylation Source: UniProtKB
  • protein glycosylation Source: UniProtKB

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferaseBy similarity (EC:2.4.1.256By similarity)
Alternative name(s):
Alpha-1,2-glucosyltransferase ALG10-A
Alpha-2-glucosyltransferase ALG10-B
Asparagine-linked glycosylation protein 10 homolog B
Potassium channel regulator 1
Gene namesi
Name:ALG10B
Synonyms:KCR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000175548.8
HGNCiHGNC:31088 ALG10B
MIMi603313 gene
neXtProtiNX_Q5I7T1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Topological domaini28 – 64ExtracellularSequence analysisAdd BLAST37
Transmembranei65 – 85HelicalSequence analysisAdd BLAST21
Topological domaini86 – 97CytoplasmicSequence analysisAdd BLAST12
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 126ExtracellularSequence analysis8
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Topological domaini148 – 150CytoplasmicSequence analysis3
Transmembranei151 – 171HelicalSequence analysisAdd BLAST21
Topological domaini172 – 175ExtracellularSequence analysis4
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 256CytoplasmicSequence analysisAdd BLAST60
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21
Topological domaini278 – 283ExtracellularSequence analysis6
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Topological domaini305 – 317CytoplasmicSequence analysisAdd BLAST13
Transmembranei318 – 338HelicalSequence analysisAdd BLAST21
Topological domaini339 – 365ExtracellularSequence analysisAdd BLAST27
Transmembranei366 – 386HelicalSequence analysisAdd BLAST21
Topological domaini387 – 392CytoplasmicSequence analysis6
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 436ExtracellularSequence analysisAdd BLAST23
Transmembranei437 – 457HelicalSequence analysisAdd BLAST21
Topological domaini458 – 473CytoplasmicSequence analysisAdd BLAST16

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi144245
OpenTargetsiENSG00000175548
PharmGKBiPA134936082

Polymorphism and mutation databases

BioMutaiALG10B
DMDMi296434391

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002154481 – 473Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferaseAdd BLAST473

Proteomic databases

EPDiQ5I7T1
MaxQBiQ5I7T1
PaxDbiQ5I7T1
PeptideAtlasiQ5I7T1
PRIDEiQ5I7T1

PTM databases

iPTMnetiQ5I7T1
PhosphoSitePlusiQ5I7T1

Expressioni

Tissue specificityi

Highly expressed in heart, placenta, liver, kidney and pancreas. Weakly expressed in lung, skeletal muscle and brain.1 Publication

Gene expression databases

BgeeiENSG00000175548
CleanExiHS_ALG10B
ExpressionAtlasiQ5I7T1 baseline and differential
GenevisibleiQ5I7T1 HS

Organism-specific databases

HPAiHPA043329
HPA063387

Interactioni

Subunit structurei

Interacts with KCNH1 and KCNH2.By similarity

Protein-protein interaction databases

BioGridi126840, 6 interactors
STRINGi9606.ENSP00000310120

Structurei

3D structure databases

ProteinModelPortaliQ5I7T1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALG10 glucosyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2642 Eukaryota
ENOG410YGXE LUCA
GeneTreeiENSGT00390000012906
HOGENOMiHOG000240902
HOVERGENiHBG053222
InParanoidiQ5I7T1
KOiK03850
OMAiEATPRSN
OrthoDBiEOG091G0A27
PhylomeDBiQ5I7T1
TreeFamiTF300150

Family and domain databases

InterProiView protein in InterPro
IPR016900 Alg10
PANTHERiPTHR12989 PTHR12989, 1 hit
PfamiView protein in Pfam
PF04922 DIE2_ALG10, 1 hit
PIRSFiPIRSF028810 Alpha1_2_glucosyltferase_Alg10, 1 hit

Sequencei

Sequence statusi: Complete.

Q5I7T1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLEGYCFS AALSCTFLVS CLLFSAFSRA LREPYMDEIF HLPQAQRYCE
60 70 80 90 100
GHFSLSQWDP MITTLPGLYL VSVGVVKPAI WIFAWSEHVV CSIGMLRFVN
110 120 130 140 150
LLFSVGNFYL LYLLFHKVQP RNKAASSIQR VLSTLTLAVF PTLYFFNFLY
160 170 180 190 200
YTEAGSMFFT LFAYLMCLYG NHKTSAFLGF CGFMFRQTNI IWAVFCAGNV
210 220 230 240 250
IAQKLTEAWK TELQKKEDRL PPIKGPFAEF RKILQFLLAY SMSFKNLSML
260 270 280 290 300
FCLTWPYILL GFLFCAFVVV NGGIVIGDRS SHEACLHFPQ LFYFFSFTLF
310 320 330 340 350
FSFPHLLSPS KIKTFLSLVW KHGILFLVVT LVSVFLVWKF TYAHKYLLAD
360 370 380 390 400
NRHYTFYVWK RVFQRYAILK YLLVPAYIFA GWSIADSLKS KPIFWNLMFF
410 420 430 440 450
ICLFIVIVPQ KLLEFRYFIL PYVIYRLNIT LPPTSRLVCE LSCYAIVNFI
460 470
TFYIFLNKTF QWPNSQDIQR FMW
Length:473
Mass (Da):55,448
Last modified:May 18, 2010 - v2
Checksum:i47D0CF7B6C84B8EC
GO

Polymorphismi

Genetic variations in ALG10B may reduce susceptibility to acquired long QT syndrome.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04821784A → G3 PublicationsCorresponds to variant dbSNP:rs6582584Ensembl.1
Natural variantiVAR_061002383S → N. Corresponds to variant dbSNP:rs57963306Ensembl.1
Natural variantiVAR_023753446I → V in some patients with drug-induced cardiac repolarization defects; exerts a greater protective effect, relative to wild-type, against drug blockage of KCNH2 potassium channel. 1 PublicationCorresponds to variant dbSNP:rs61730283Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY845858 mRNA Translation: AAW31756.1
AC117372 Genomic DNA No translation available.
CH471111 Genomic DNA Translation: EAW57795.1
BC137413 mRNA Translation: AAI37414.1
BC137414 mRNA Translation: AAI37415.1
CCDSiCCDS31772.1
RefSeqiNP_001013642.1, NM_001013620.3
UniGeneiHs.259305

Genome annotation databases

EnsembliENST00000308742; ENSP00000310120; ENSG00000175548
GeneIDi144245
KEGGihsa:144245
UCSCiuc001rln.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiAG10B_HUMAN
AccessioniPrimary (citable) accession number: Q5I7T1
Secondary accession number(s): B2RPF4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: May 18, 2010
Last modified: March 28, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health