Reviewed, UniProtKB/Swiss-Prot Q5I6M2 (HOG1_SETTU)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase HOG1
      Short name=MAP kinase HOG1
    EC=2.7.11.24
Alternative name(s):
    MAP kinase STK1

Gene names

Name:	HOG1
Synonyms:	STK1

Organism

Setosphaeria turcica (Northern corn leaf blight disease fungus)

Taxonomic identifier

93612 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporaceae › Setosphaeria

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-144 and Tyr-146, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Mitogen-activated protein kinase HOG1		PRO_0000289701
Regions
	Domain	1 – 272	272	Protein kinase
	Nucleotide binding	1 – 7	7	ATP By similarity
	Motif	144 – 146	3	TXY
Sites
	Active site	114	1	Proton acceptor By similarity
	Binding site	22	1	ATP By similarity
Amino acid modifications
	Modified residue	144	1	Phosphothreonine By similarity
	Modified residue	146	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5I6M2-1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 0AD4C21C0BA4E346	FASTA	329	37,743
	10 20 30 40 50 60 MGAFGLVCSA KDQLTNQAVA VKKIMKPFST PVLSKRTYRE LKLLKHLRHE NIISLSDIFI 70 80 90 100 110 120 SPLEDIYFVT ELLGTDLHRL LTSRPLEKQF IQYFLYQILR GLKYIHSAGV VHRDLKPSNI 130 140 150 160 170 180 LVNENCDLKI CDFGLARIQD PQVTGYVSTR YYRAPEIMLT WQKYDVEVDI WSAGCIFAEM 190 200 210 220 230 240 LEGKPLFPGK DHVNQFSIIT ELLGTPPDDV IQTICSENTL RFVQPLPKRE RQPLANKFKN 250 260 270 280 290 300 AEPEAVDLLE NMLVFDPRKR VRAEQALAHP YLAPYHDPTD EPIAEEKFDW SFNDADLPVD 310 320 TWKIMMYSEI LDYHNVDAAA QDAPESNGS

References

[1]	Gu S., Fan Y., Dong J. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY849317 mRNA. Translation: AAW55999.2.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.24. 291933.
Family and domain databases
InterPro	IPR003527. MAP_kinase_CS. IPR008352. MAPK_p38. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01773. P38MAPKINASE.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOG1_SETTU

Accession

Primary (citable) accession number: Q5I6M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 31, 2006
Last modified:	September 22, 2009
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)