Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q5I6B8 (PI51C_RAT)

Last modified February 9, 2010. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma
      Short name=PtdIns(4)P-5-kinase 1 gamma
      Short name=PIP5K1-gamma
    EC=2.7.1.68
Alternative name(s):
    Phosphatidylinositol-4-phosphate 5-kinase type I gamma
      Short name=PIP5KIgamma
Gene names
Name: Pip5k1c
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length688 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Mediates RAC1-dependent reorganization of actin filaments By similarity. Plays a role in membrane ruffling and assembly of clathrin-coated pits at the synapse. Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Ref.2

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Enzyme regulation

Activated by interaction with ARF6.

Subunit structure

Interacts with TLN1, TLN2 and CSK By similarity. Interacts with ARF6. Interacts with AP2B1. Interacts with AP2M1; phosphorylation of PIP5K1C by CSK disrupts the interaction; clathrin competes with PIP5K1C By similarity. Ref.2

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic, associated with the plasma membrane. Detected in focal adhesion plaques, membrane ruffles and plasma membrane invaginations. Ref.2

Sequence similarities

Contains 1 PIPK domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 688688Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma
PRO_0000185464

Regions

Domain75 – 443369PIPK

Amino acid modifications

Modified residue6711Phosphotyrosine; by CSK By similarity

Experimental info

Mutagenesis1881K → A: Reduces activity by over 99%. Ref.1
Mutagenesis3161D → K: Loss of kinase activity. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q5I6B8-1 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: F34E175EFFF54D5D

FASTA68875,594
        10         20         30         40         50         60 
MELEVPDEAE SAEAGAVTAE AAWSAESGAA AGMTQKKAIL AEAPLVTGQP GPGHGKKLGH 

        70         80         90        100        110        120 
RGVDASGETT YKKTTSSTLK GAIQLGIGYT VGNLSSKPER DVLMQDFYVV ESIFFPSEGS 

       130        140        150        160        170        180 
NLTPAHHFQD FRFKTYAPVA FRYFRELFGI RPDDYLYSLC NEPLIELSNP GASGSVFYVT 

       190        200        210        220        230        240 
SDDEFIIKTV MHKEAEFLQK LLPGYYMNLN QNPRTLLPKF YGLYCVQSGG KNIRVVVMNN 

       250        260        270        280        290        300 
VLPRVVKMHL KFDLKGSTYK RRASKKEKEK SLPTYKDLDF MQDMPEGLLL DSDTFGALVK 

       310        320        330        340        350        360 
TLQRDCLVLE SFKIMDYSLL LGVHNIDQQE RERQAEGAQS KADEKRPVAQ KALYSTARES 

       370        380        390        400        410        420 
IQGGAARGEA IETDDTMGGI PAVNGRGERL LLHIGIIDIL QSYRFIKKLE HTWKALVHDG 

       430        440        450        460        470        480 
DTVSVHRPSF YAERFFKFMS STVFRKSSSL KSSPSKKGRG ALLAVKPLGP TAAFSASQIP 

       490        500        510        520        530        540 
SEREDVQYDL RGARSYPTLE DEGRPDLLPC TPPSFEEATT ASIATTLSST SLSIPERSPS 

       550        560        570        580        590        600 
DTSEQPRYRR RTQSSGQDGR PQEELHAEDL QKITVQVEPV CGVGVVVPKE QGAGVEVPPS 

       610        620        630        640        650        660 
GASAAATVEV DAASQASEPA SQASDEEDAP STDIYFFAHG RYWLFSPRRR RLRAVTPSHT 

       670        680 
GAPTDGRSWV YSPLHYSARP ASDGESDT 

« Hide

References

[1]"A novel neuronal-specific splice variant of Type I phosphatidylinositol 4-phosphate 5-kinase isoform gamma."
Giudici M.-L., Emson P.C., Irvine R.F.
Biochem. J. 379:489-496(2004) [PubMed: 14741049] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF LYS-188 AND ASP-316, TISSUE SPECIFICITY.
Tissue: Hippocampus.
[2]"ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Igamma."
Krauss M., Kinuta M., Wenk M.R., De Camilli P., Takei K., Haucke V.
J. Cell Biol. 162:113-124(2003) [PubMed: 12847086] [Abstract]
Cited for: INTERACTION WITH ARF6, SUBCELLULAR LOCATION, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY850259 mRNA. Translation: AAW34235.1.
IPIIPI00389614.
RefSeqNP_001009967.2.
NP_001029142.1.
UniGeneRn.2928

3D structure databases

SMRQ5I6B8. Positions 75-442.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5I6B8.

Genome annotation databases

EnsemblENSRNOT00000061159; ENSRNOP00000057873; ENSRNOG00000020577; Rattus norvegicus. [Genome view]
GeneID314641.
KEGGrno:314641.

Organism-specific databases

CTD314641.
RGD1309938. Pip5k1c.

Phylogenomic databases

HOVERGENQ5I6B8.
InParanoidQ5I6B8.

Enzyme and pathway databases

BRENDA2.7.1.68. 248.

Gene expression databases

GenevestigatorQ5I6B8.

Family and domain databases

InterProIPR002498. PInositol-4-P-5-kinase_core.
IPR016034. PInositol-4P-5-kinase_core_sub.
[Graphical view]
PANTHERPTHR23086. PIP5K. 1 hit.
PfamPF01504. PIP5K. 1 hit.
[Graphical view]
SMARTSM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEPS51455. PIPK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio668018.

Entry information

Entry namePI51C_RAT
AccessionPrimary (citable) accession number: Q5I6B8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: February 15, 2005
Last modified: February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents