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Q5I598 (MTHR_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate reductase

EC=1.5.1.20
Gene names
Name:MTHFR
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length655 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine By similarity.

Catalytic activity

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactor

FAD By similarity.

Enzyme regulation

Allosterically regulated by S-adenosylmethionine By similarity.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the methylenetetrahydrofolate reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 655655Methylenetetrahydrofolate reductase
PRO_0000236275

Regions

Nucleotide binding62 – 676NAD By similarity
Nucleotide binding93 – 942NAD and FAD By similarity
Nucleotide binding156 – 1583FAD By similarity
Nucleotide binding173 – 1742FAD By similarity
Nucleotide binding200 – 2034FAD By similarity

Sites

Active site621Proton donor/acceptor By similarity
Binding site1261FAD By similarity
Binding site1581Substrate By similarity
Binding site1961FAD By similarity
Binding site2091FAD By similarity
Binding site2161FAD By similarity
Binding site2271Substrate By similarity
Binding site3201Substrate By similarity
Binding site3241Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5I598 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 8F88D084036BD3A5

FASTA65574,485
        10         20         30         40         50         60 
MVNEPRGNGS PGPRWEGSSS GSESSRTSSR CSTPGLDPER CERLREKMKR KMDSGDKWFS 

        70         80         90        100        110        120 
LEFFPPRTAQ GAVNLISRFD RMGAGGPLFV DVTWHPAGDP GSDKETSSMV IASTAVNYCG 

       130        140        150        160        170        180 
LETILHMTCC HQSREEITGH LNKAKQLGLK NILALRGDPI GDQWEEEEGG FNYATDLVKH 

       190        200        210        220        230        240 
IRNEFGDYFD VCVAGYPKGH PEGESFEADL KHLKEKVAAG ADFIITQLFF EAETFFRFVK 

       250        260        270        280        290        300 
ACSEIGITCP VLPGIFPIQG YHSLRQLVKL SKLEVPQQIK DVIEPIKDND AAIRNYGIEQ 

       310        320        330        340        350        360 
AVSLCQELLA SGLVPGLHFY TLNREVATIE VLKRLGLWIE DPRRPLPWAL SAHPKRRVED 

       370        380        390        400        410        420 
VRPIFWASRP KSYIYRTQEW DEFPNGRWGN SSSPAFGELK DYYLFYLKSK SPKEELLKMW 

       430        440        450        460        470        480 
GEELTSEESV FQVFAHHLSG EPNQNGYKVT CLPWNDEPLA AETSLMKEEL LRVNRRGILT 

       490        500        510        520        530        540 
INSQPNINGK PSSDPIVGWG PSGGYVFQKA YLEFFTSRET VEALLQVLKK YELRVNYHIV 

       550        560        570        580        590        600 
DVKGENITNA PELQPNAVTW GIFPGREIIQ PTVVDPVSFM FWKDEAFALW IEQWGKLYEE 

       610        620        630        640        650 
ESPSRMIIQY IHDNYFLVNL VDNEFPLDNC LWQVVEDTFE LLSRPPQDKR ETEAL 

« Hide

References

[1]"Interactions folic acid-vitamin B12-methionine: effects on liver metabolism and production of dairy cows."
Charest R., Beaudry D., Girard C., Palin M.-F.
Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY854631 mRNA. Translation: AAW39033.1.
RefSeqNP_001011685.1. NM_001011685.1.
UniGeneBt.26830.

3D structure databases

ProteinModelPortalQ5I598.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000052746.

Proteomic databases

PRIDEQ5I598.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID497032.
KEGGbta:497032.

Organism-specific databases

CTD4524.

Phylogenomic databases

eggNOGCOG0685.
HOGENOMHOG000246234.
HOVERGENHBG006414.
InParanoidQ5I598.
KOK00297.

Enzyme and pathway databases

UniPathwayUPA00193.

Family and domain databases

Gene3D3.20.20.220. 1 hit.
InterProIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMSSF51730. SSF51730. 1 hit.
TIGRFAMsTIGR00677. fadh2_euk. 1 hit.
ProtoNetSearch...

Other

NextBio20865843.

Entry information

Entry nameMTHR_BOVIN
AccessionPrimary (citable) accession number: Q5I598
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: February 15, 2005
Last modified: June 11, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways