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Q5I598

- MTHR_BOVIN

UniProt

Q5I598 - MTHR_BOVIN

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Protein

Methylenetetrahydrofolate reductase

Gene

MTHFR

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine.By similarity

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD.By similarity

Enzyme regulationi

Allosterically regulated by S-adenosylmethionine.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei62 – 621Proton donor/acceptorBy similarity
Binding sitei126 – 1261FADBy similarity
Binding sitei158 – 1581SubstrateBy similarity
Binding sitei196 – 1961FADBy similarity
Binding sitei209 – 2091FADBy similarity
Binding sitei216 – 2161FADBy similarity
Binding sitei227 – 2271SubstrateBy similarity
Binding sitei320 – 3201SubstrateBy similarity
Binding sitei324 – 3241SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi62 – 676NADBy similarity
Nucleotide bindingi93 – 942NAD and FADBy similarity
Nucleotide bindingi156 – 1583FADBy similarity
Nucleotide bindingi173 – 1742FADBy similarity
Nucleotide bindingi200 – 2034FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine metabolic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:MTHFR
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 655655Methylenetetrahydrofolate reductasePRO_0000236275Add
BLAST

Proteomic databases

PRIDEiQ5I598.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000052746.

Structurei

3D structure databases

ProteinModelPortaliQ5I598.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246234.
HOVERGENiHBG006414.
InParanoidiQ5I598.
KOiK00297.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5I598-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNEPRGNGS PGPRWEGSSS GSESSRTSSR CSTPGLDPER CERLREKMKR
60 70 80 90 100
KMDSGDKWFS LEFFPPRTAQ GAVNLISRFD RMGAGGPLFV DVTWHPAGDP
110 120 130 140 150
GSDKETSSMV IASTAVNYCG LETILHMTCC HQSREEITGH LNKAKQLGLK
160 170 180 190 200
NILALRGDPI GDQWEEEEGG FNYATDLVKH IRNEFGDYFD VCVAGYPKGH
210 220 230 240 250
PEGESFEADL KHLKEKVAAG ADFIITQLFF EAETFFRFVK ACSEIGITCP
260 270 280 290 300
VLPGIFPIQG YHSLRQLVKL SKLEVPQQIK DVIEPIKDND AAIRNYGIEQ
310 320 330 340 350
AVSLCQELLA SGLVPGLHFY TLNREVATIE VLKRLGLWIE DPRRPLPWAL
360 370 380 390 400
SAHPKRRVED VRPIFWASRP KSYIYRTQEW DEFPNGRWGN SSSPAFGELK
410 420 430 440 450
DYYLFYLKSK SPKEELLKMW GEELTSEESV FQVFAHHLSG EPNQNGYKVT
460 470 480 490 500
CLPWNDEPLA AETSLMKEEL LRVNRRGILT INSQPNINGK PSSDPIVGWG
510 520 530 540 550
PSGGYVFQKA YLEFFTSRET VEALLQVLKK YELRVNYHIV DVKGENITNA
560 570 580 590 600
PELQPNAVTW GIFPGREIIQ PTVVDPVSFM FWKDEAFALW IEQWGKLYEE
610 620 630 640 650
ESPSRMIIQY IHDNYFLVNL VDNEFPLDNC LWQVVEDTFE LLSRPPQDKR

ETEAL
Length:655
Mass (Da):74,485
Last modified:February 15, 2005 - v1
Checksum:i8F88D084036BD3A5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY854631 mRNA. Translation: AAW39033.1.
RefSeqiNP_001011685.1. NM_001011685.1.
UniGeneiBt.26830.

Genome annotation databases

GeneIDi497032.
KEGGibta:497032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY854631 mRNA. Translation: AAW39033.1 .
RefSeqi NP_001011685.1. NM_001011685.1.
UniGenei Bt.26830.

3D structure databases

ProteinModelPortali Q5I598.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000052746.

Proteomic databases

PRIDEi Q5I598.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 497032.
KEGGi bta:497032.

Organism-specific databases

CTDi 4524.

Phylogenomic databases

eggNOGi COG0685.
HOGENOMi HOG000246234.
HOVERGENi HBG006414.
InParanoidi Q5I598.
KOi K00297.

Enzyme and pathway databases

UniPathwayi UPA00193 .

Miscellaneous databases

NextBioi 20865843.

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Interactions folic acid-vitamin B12-methionine: effects on liver metabolism and production of dairy cows."
    Charest R., Beaudry D., Girard C., Palin M.-F.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiMTHR_BOVIN
AccessioniPrimary (citable) accession number: Q5I598
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: February 15, 2005
Last modified: October 1, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3