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Protein

Methylenetetrahydrofolate reductase

Gene

MTHFR

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine.By similarity

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Enzyme regulationi

Allosterically regulated by S-adenosylmethionine.By similarity

Pathway: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei62 – 621Proton donor/acceptorBy similarity
Binding sitei126 – 1261FADBy similarity
Binding sitei158 – 1581SubstrateBy similarity
Binding sitei196 – 1961FADBy similarity
Binding sitei209 – 2091FADBy similarity
Binding sitei216 – 2161FADBy similarity
Binding sitei227 – 2271SubstrateBy similarity
Binding sitei320 – 3201SubstrateBy similarity
Binding sitei324 – 3241SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi62 – 676NADBy similarity
Nucleotide bindingi93 – 942NAD and FADBy similarity
Nucleotide bindingi156 – 1583FADBy similarity
Nucleotide bindingi173 – 1742FADBy similarity
Nucleotide bindingi200 – 2034FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BRENDAi1.5.1.20. 908.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:MTHFR
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 655655Methylenetetrahydrofolate reductasePRO_0000236275Add
BLAST

Proteomic databases

PRIDEiQ5I598.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027586.

Structurei

3D structure databases

ProteinModelPortaliQ5I598.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246234.
HOVERGENiHBG006414.
InParanoidiQ5I598.
KOiK00297.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5I598-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNEPRGNGS PGPRWEGSSS GSESSRTSSR CSTPGLDPER CERLREKMKR
60 70 80 90 100
KMDSGDKWFS LEFFPPRTAQ GAVNLISRFD RMGAGGPLFV DVTWHPAGDP
110 120 130 140 150
GSDKETSSMV IASTAVNYCG LETILHMTCC HQSREEITGH LNKAKQLGLK
160 170 180 190 200
NILALRGDPI GDQWEEEEGG FNYATDLVKH IRNEFGDYFD VCVAGYPKGH
210 220 230 240 250
PEGESFEADL KHLKEKVAAG ADFIITQLFF EAETFFRFVK ACSEIGITCP
260 270 280 290 300
VLPGIFPIQG YHSLRQLVKL SKLEVPQQIK DVIEPIKDND AAIRNYGIEQ
310 320 330 340 350
AVSLCQELLA SGLVPGLHFY TLNREVATIE VLKRLGLWIE DPRRPLPWAL
360 370 380 390 400
SAHPKRRVED VRPIFWASRP KSYIYRTQEW DEFPNGRWGN SSSPAFGELK
410 420 430 440 450
DYYLFYLKSK SPKEELLKMW GEELTSEESV FQVFAHHLSG EPNQNGYKVT
460 470 480 490 500
CLPWNDEPLA AETSLMKEEL LRVNRRGILT INSQPNINGK PSSDPIVGWG
510 520 530 540 550
PSGGYVFQKA YLEFFTSRET VEALLQVLKK YELRVNYHIV DVKGENITNA
560 570 580 590 600
PELQPNAVTW GIFPGREIIQ PTVVDPVSFM FWKDEAFALW IEQWGKLYEE
610 620 630 640 650
ESPSRMIIQY IHDNYFLVNL VDNEFPLDNC LWQVVEDTFE LLSRPPQDKR

ETEAL
Length:655
Mass (Da):74,485
Last modified:February 15, 2005 - v1
Checksum:i8F88D084036BD3A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY854631 mRNA. Translation: AAW39033.1.
RefSeqiNP_001011685.1. NM_001011685.1.
UniGeneiBt.26830.

Genome annotation databases

GeneIDi497032.
KEGGibta:497032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY854631 mRNA. Translation: AAW39033.1.
RefSeqiNP_001011685.1. NM_001011685.1.
UniGeneiBt.26830.

3D structure databases

ProteinModelPortaliQ5I598.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027586.

Proteomic databases

PRIDEiQ5I598.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi497032.
KEGGibta:497032.

Organism-specific databases

CTDi4524.

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246234.
HOVERGENiHBG006414.
InParanoidiQ5I598.
KOiK00297.

Enzyme and pathway databases

UniPathwayiUPA00193.
BRENDAi1.5.1.20. 908.

Miscellaneous databases

NextBioi20865843.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Interactions folic acid-vitamin B12-methionine: effects on liver metabolism and production of dairy cows."
    Charest R., Beaudry D., Girard C., Palin M.-F.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiMTHR_BOVIN
AccessioniPrimary (citable) accession number: Q5I598
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: February 15, 2005
Last modified: June 24, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.