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Q5I598

- MTHR_BOVIN

UniProt

Q5I598 - MTHR_BOVIN

Protein

Methylenetetrahydrofolate reductase

Gene

MTHFR

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine.By similarity

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Enzyme regulationi

    Allosterically regulated by S-adenosylmethionine.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei62 – 621Proton donor/acceptorBy similarity
    Binding sitei126 – 1261FADBy similarity
    Binding sitei158 – 1581SubstrateBy similarity
    Binding sitei196 – 1961FADBy similarity
    Binding sitei209 – 2091FADBy similarity
    Binding sitei216 – 2161FADBy similarity
    Binding sitei227 – 2271SubstrateBy similarity
    Binding sitei320 – 3201SubstrateBy similarity
    Binding sitei324 – 3241SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi62 – 676NADBy similarity
    Nucleotide bindingi93 – 942NAD and FADBy similarity
    Nucleotide bindingi156 – 1583FADBy similarity
    Nucleotide bindingi173 – 1742FADBy similarity
    Nucleotide bindingi200 – 2034FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine metabolic process Source: InterPro
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Name:MTHFR
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 655655Methylenetetrahydrofolate reductasePRO_0000236275Add
    BLAST

    Proteomic databases

    PRIDEiQ5I598.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000052746.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5I598.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000246234.
    HOVERGENiHBG006414.
    InParanoidiQ5I598.
    KOiK00297.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5I598-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVNEPRGNGS PGPRWEGSSS GSESSRTSSR CSTPGLDPER CERLREKMKR    50
    KMDSGDKWFS LEFFPPRTAQ GAVNLISRFD RMGAGGPLFV DVTWHPAGDP 100
    GSDKETSSMV IASTAVNYCG LETILHMTCC HQSREEITGH LNKAKQLGLK 150
    NILALRGDPI GDQWEEEEGG FNYATDLVKH IRNEFGDYFD VCVAGYPKGH 200
    PEGESFEADL KHLKEKVAAG ADFIITQLFF EAETFFRFVK ACSEIGITCP 250
    VLPGIFPIQG YHSLRQLVKL SKLEVPQQIK DVIEPIKDND AAIRNYGIEQ 300
    AVSLCQELLA SGLVPGLHFY TLNREVATIE VLKRLGLWIE DPRRPLPWAL 350
    SAHPKRRVED VRPIFWASRP KSYIYRTQEW DEFPNGRWGN SSSPAFGELK 400
    DYYLFYLKSK SPKEELLKMW GEELTSEESV FQVFAHHLSG EPNQNGYKVT 450
    CLPWNDEPLA AETSLMKEEL LRVNRRGILT INSQPNINGK PSSDPIVGWG 500
    PSGGYVFQKA YLEFFTSRET VEALLQVLKK YELRVNYHIV DVKGENITNA 550
    PELQPNAVTW GIFPGREIIQ PTVVDPVSFM FWKDEAFALW IEQWGKLYEE 600
    ESPSRMIIQY IHDNYFLVNL VDNEFPLDNC LWQVVEDTFE LLSRPPQDKR 650
    ETEAL 655
    Length:655
    Mass (Da):74,485
    Last modified:February 15, 2005 - v1
    Checksum:i8F88D084036BD3A5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY854631 mRNA. Translation: AAW39033.1.
    RefSeqiNP_001011685.1. NM_001011685.1.
    UniGeneiBt.26830.

    Genome annotation databases

    GeneIDi497032.
    KEGGibta:497032.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY854631 mRNA. Translation: AAW39033.1 .
    RefSeqi NP_001011685.1. NM_001011685.1.
    UniGenei Bt.26830.

    3D structure databases

    ProteinModelPortali Q5I598.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000052746.

    Proteomic databases

    PRIDEi Q5I598.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 497032.
    KEGGi bta:497032.

    Organism-specific databases

    CTDi 4524.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000246234.
    HOVERGENi HBG006414.
    InParanoidi Q5I598.
    KOi K00297.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .

    Miscellaneous databases

    NextBioi 20865843.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Interactions folic acid-vitamin B12-methionine: effects on liver metabolism and production of dairy cows."
      Charest R., Beaudry D., Girard C., Palin M.-F.
      Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.

    Entry informationi

    Entry nameiMTHR_BOVIN
    AccessioniPrimary (citable) accession number: Q5I598
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3