Reviewed, UniProtKB/Swiss-Prot Q5I598 (MTHR_BOVIN)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate reductase
    EC=1.5.1.20

Gene names

Name:

MTHFR

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	655 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine By similarity.
Catalytic activity	5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Enzyme regulation	Allosterically regulated by S-adenosylmethionine By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 655	655	Methylenetetrahydrofolate reductase		PRO_0000236275

Sequences

	Sequence		Length	Mass (Da)
	Q5I598-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 8F88D084036BD3A5	FASTA	655	74,485
	10 20 30 40 50 60 MVNEPRGNGS PGPRWEGSSS GSESSRTSSR CSTPGLDPER CERLREKMKR KMDSGDKWFS 70 80 90 100 110 120 LEFFPPRTAQ GAVNLISRFD RMGAGGPLFV DVTWHPAGDP GSDKETSSMV IASTAVNYCG 130 140 150 160 170 180 LETILHMTCC HQSREEITGH LNKAKQLGLK NILALRGDPI GDQWEEEEGG FNYATDLVKH 190 200 210 220 230 240 IRNEFGDYFD VCVAGYPKGH PEGESFEADL KHLKEKVAAG ADFIITQLFF EAETFFRFVK 250 260 270 280 290 300 ACSEIGITCP VLPGIFPIQG YHSLRQLVKL SKLEVPQQIK DVIEPIKDND AAIRNYGIEQ 310 320 330 340 350 360 AVSLCQELLA SGLVPGLHFY TLNREVATIE VLKRLGLWIE DPRRPLPWAL SAHPKRRVED 370 380 390 400 410 420 VRPIFWASRP KSYIYRTQEW DEFPNGRWGN SSSPAFGELK DYYLFYLKSK SPKEELLKMW 430 440 450 460 470 480 GEELTSEESV FQVFAHHLSG EPNQNGYKVT CLPWNDEPLA AETSLMKEEL LRVNRRGILT 490 500 510 520 530 540 INSQPNINGK PSSDPIVGWG PSGGYVFQKA YLEFFTSRET VEALLQVLKK YELRVNYHIV 550 560 570 580 590 600 DVKGENITNA PELQPNAVTW GIFPGREIIQ PTVVDPVSFM FWKDEAFALW IEQWGKLYEE 610 620 630 640 650 ESPSRMIIQY IHDNYFLVNL VDNEFPLDNC LWQVVEDTFE LLSRPPQDKR ETEAL

References

[1]	"Interactions folic acid-vitamin B12-methionine: effects on liver metabolism and production of dairy cows." Charest R., Beaudry D., Girard C., Palin M.-F. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY854631 mRNA. Translation: AAW39033.1.
RefSeq	NP_001011685.1.
UniGene	Bt.26830
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000020698. Bos taurus. [Contig view]
GeneID	497032.
KEGG	bta:497032.
Phylogenomic databases
HOVERGEN	Q5I598.
Family and domain databases
InterPro	IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00677. fadh2_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTHR_BOVIN

Accession

Primary (citable) accession number: Q5I598

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	February 15, 2005
Last modified:	November 25, 2008
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)