Reviewed, UniProtKB/Swiss-Prot Q5I597 (BHMT1_BOVIN)

Names and origin

Protein names

Recommended name:
    Betaine--homocysteine S-methyltransferase 1
    EC=2.1.1.5

Gene names

Name:

BHMT

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline By similarity.
Catalytic activity	Trimethylammonioacetate + L-homocysteine = dimethylglycine + L-methionine.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 Hcy-binding domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	betaine-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Betaine--homocysteine S-methyltransferase 1		PRO_0000236274
Regions
	Domain	11 – 314	304	Hcy-binding
Sites
	Metal binding	217	1	Zinc By similarity
	Metal binding	299	1	Zinc By similarity
	Metal binding	300	1	Zinc By similarity
Experimental info
	Sequence conflict	216	1	N → K in AAI12600. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5I597-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 101C4070BAA30096	FASTA	407	44,878
	10 20 30 40 50 60 MAPAGGKNVK KGILERLNSG EVIIGDGGFV FALEKRGYVK AGPWTPEAAV EHPEAVRQLH 70 80 90 100 110 120 REFLRAGSNV MQTFTFYASE DKLENRGNYV AEKISGQKVN EAACDIARQV ADEGDALVAG 130 140 150 160 170 180 GVSQTPSYLS CKSETEVKKV FQQQLEVFVK KNVDFLIAEY FEHVEEAVWA VEALKASGKP 190 200 210 220 230 240 VAATMCIGPE GDLHSVTPGE CAVRLVKAGA SIVGVNCHFD PTISLQTVKL MKEGLEAAGL 250 260 270 280 290 300 KAHLMSQPLA YHTPDCGKQG FIDLPEFPFG LEPRVATRWD IQKYAREAYN LGVRYIGGCC 310 320 330 340 350 360 GFEPYHIRAI AEELAPERGF LPLASEKHGS WGSGLDMHTK PWIRARARKE YWENLQIASG 370 380 390 400 RPYNPSMSKP DAWGVTKGTA ELMQQKEATT EQQLRELFEK QKFKSAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interactions folic acid-vitamin B12-methionine: effects on liver metabolism and production of dairy cows." Charest R., Beaudry D., Girard C., Palin M.-F. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY854632 mRNA. Translation: AAW39034.1. BC112599 mRNA. Translation: AAI12600.1.
IPI	IPI00687917.
RefSeq	NP_001011679.1.
UniGene	Bt.16399
3D structure databases
SMR	Q5I597. Positions 11-371.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000002916; ENSBTAP00000002916; ENSBTAG00000002255; Bos taurus. [Genome view]
GeneID	497025.
KEGG	bta:497025.
Organism-specific databases
CTD	497025.
Phylogenomic databases
HOVERGEN	Q5I597.
OMA	CKSEAEV.
Enzyme and pathway databases
BRENDA	2.1.1.5. 251.
Family and domain databases
InterPro	IPR017226. Betaine-hCys_S-MeTrfase_BHMT. IPR003726. S_MeTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.330. S_methyl_trans. 1 hit.
Pfam	PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF037505. Betaine_HMT. 1 hit.
PROSITE	PS50970. HCY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BHMT1_BOVIN

Accession

Primary (citable) accession number: Q5I597
Secondary accession number(s): Q2KIL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	February 15, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)