Q5I3B2 (IOD2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type II iodothyronine deiodinase
EC=1.97.1.10

Alternative name(s):
5DII
DIOII
Type 2 DI
Type-II 5'-deiodinase

Gene names

Name:

DIO2

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development By similarity.
Catalytic activity	3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.
Subunit structure	Interacts with USP20 and USP33. Interacts with MARCH6 By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Highly expressed in thyroid, mammary and pituitary glands, then in hypothalamus. Low levels detected in diaphragm, heart, kidney and lung. Ref.1
Post-translational modification	Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33 By similarity.
Sequence similarities	Belongs to the iodothyronine deiodinase family.

Ontologies

Keywords
Biological process	Thyroid hormones biosynthesis
Cellular component	Membrane
Coding sequence diversity	Selenocysteine
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW thyroid hormone metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	thyroxine 5'-deiodinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Type II iodothyronine deiodinase		PRO_0000223865
Regions
	Transmembrane	10 – 34	25	Helical; Potential
Sites
	Active site	133	1
Amino acid modifications
	Non-standard residue	133	1	Selenocysteine
	Non-standard residue	266	1	Selenocysteine

Sequences

	Sequence		Length	Mass (Da)
	Q5I3B2 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 6644027D04BA74EA	FASTA	269	30,303
	10 20 30 40 50 60 MGILSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVVLL LSRSKSTRGQ WRRMLTSEGM 70 80 90 100 110 120 RCIWKSFLLD AYKQVKLGED APNSSVVHVS SPEGGDTSGN GAQEKTVDGT ECHLLDFASP 130 140 150 160 170 180 ERPLVVNFGS ATUPPFTNQL PAFSKLVEEF SSVADFLLVY IDEAHPSDGW AVPGDSSLFF 190 200 210 220 230 240 EVKKHRNQED RCAAAHQLLE RFSLPPQCRV VADRMDNNAN VAYGVAFERV CIVQRQKIAY 250 260 LGGKGPFFYN LQEVRRWLEK NFSKRUKLD

References

[1]	"Molecular cloning, expression and radiation hybrid mapping of the bovine deiodinase type II (DIO2) and deiodinase type III (DIO3) genes." Connor E.E., Laiakis E.C., Fernandes V.M., Williams J.L., Capuco A.V. Anim. Genet. 36:240-243(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Mammary epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY858551 mRNA. Translation: AAW51123.1.
IPI	IPI00924191.
RefSeq	NP_001010992.1. NM_001010992.3.
UniGene	Bt.63066.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	494548.
KEGG	bta:494548.
Organism-specific databases
CTD	1734.
Phylogenomic databases
eggNOG	NOG146259.
HOVERGEN	HBG000099.
InParanoid	Q5I3B2.
KO	K01562.
OrthoDB	EOG4X97HS.
Enzyme and pathway databases
BioCyc	CATTLE:494548-MONOMER.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR000643. Iodothyronine_deiodinase. IPR008261. Iodothyronine_deiodinase_AS. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11781. PTHR11781. 1 hit.
Pfam	PF00837. T4_deiodinase. 1 hit. [Graphical view]
PROSITE	PS01205. T4_DEIODINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20865728.

Entry information

Entry name

IOD2_BOVIN

Accession

Primary (citable) accession number: Q5I3B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	February 26, 2008
Last modified:	May 29, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program