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Protein

Type II iodothyronine deiodinase

Gene

DIO2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development (By similarity).By similarity

Catalytic activityi

3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei133 – 1331

GO - Molecular functioni

  1. thyroxine 5'-deiodinase activity Source: UniProtKB

GO - Biological processi

  1. hormone biosynthetic process Source: UniProtKB-KW
  2. thyroid hormone metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Thyroid hormones biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Type II iodothyronine deiodinase (EC:1.97.1.10)
Alternative name(s):
5DII
DIOII
Type 2 DI
Type-II 5'-deiodinase
Gene namesi
Name:DIO2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3425HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Type II iodothyronine deiodinasePRO_0000223865Add
BLAST

Post-translational modificationi

Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33 (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Expressioni

Tissue specificityi

Highly expressed in thyroid, mammary and pituitary glands, then in hypothalamus. Low levels detected in diaphragm, heart, kidney and lung.1 Publication

Interactioni

Subunit structurei

Interacts with USP20 and USP33. Interacts with MARCH6 (By similarity).By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the iodothyronine deiodinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG146259.
HOVERGENiHBG000099.
InParanoidiQ5I3B2.
KOiK17904.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11781. PTHR11781. 1 hit.
PfamiPF00837. T4_deiodinase. 1 hit.
[Graphical view]
PIRSFiPIRSF001330. IOD. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5I3B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGILSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVVLL LSRSKSTRGQ
60 70 80 90 100
WRRMLTSEGM RCIWKSFLLD AYKQVKLGED APNSSVVHVS SPEGGDTSGN
110 120 130 140 150
GAQEKTVDGT ECHLLDFASP ERPLVVNFGS ATUPPFTNQL PAFSKLVEEF
160 170 180 190 200
SSVADFLLVY IDEAHPSDGW AVPGDSSLFF EVKKHRNQED RCAAAHQLLE
210 220 230 240 250
RFSLPPQCRV VADRMDNNAN VAYGVAFERV CIVQRQKIAY LGGKGPFFYN
260
LQEVRRWLEK NFSKRUKLD
Length:269
Mass (Da):30,303
Last modified:February 26, 2008 - v2
Checksum:i6644027D04BA74EA
GO

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei133 – 1331Selenocysteine
Non-standard residuei266 – 2661Selenocysteine

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY858551 mRNA. Translation: AAW51123.1.
RefSeqiNP_001010992.1. NM_001010992.3.
UniGeneiBt.63066.

Genome annotation databases

GeneIDi494548.
KEGGibta:494548.

Keywords - Coding sequence diversityi

Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY858551 mRNA. Translation: AAW51123.1.
RefSeqiNP_001010992.1. NM_001010992.3.
UniGeneiBt.63066.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi494548.
KEGGibta:494548.

Organism-specific databases

CTDi1734.

Phylogenomic databases

eggNOGiNOG146259.
HOVERGENiHBG000099.
InParanoidiQ5I3B2.
KOiK17904.

Miscellaneous databases

NextBioi20865728.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11781. PTHR11781. 1 hit.
PfamiPF00837. T4_deiodinase. 1 hit.
[Graphical view]
PIRSFiPIRSF001330. IOD. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning, expression and radiation hybrid mapping of the bovine deiodinase type II (DIO2) and deiodinase type III (DIO3) genes."
    Connor E.E., Laiakis E.C., Fernandes V.M., Williams J.L., Capuco A.V.
    Anim. Genet. 36:240-243(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Mammary epithelium.

Entry informationi

Entry nameiIOD2_BOVIN
AccessioniPrimary (citable) accession number: Q5I3B2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: February 26, 2008
Last modified: April 1, 2015
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.