Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nibrin

Gene

nbn

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, DNA damage, DNA repair, Meiosis

Enzyme and pathway databases

ReactomeiR-DRE-2559586 DNA Damage/Telomere Stress Induced Senescence
R-DRE-5685938 HDR through Single Strand Annealing (SSA)
R-DRE-5685939 HDR through MMEJ (alt-NHEJ)
R-DRE-5685942 HDR through Homologous Recombination (HRR)
R-DRE-5693548 Sensing of DNA Double Strand Breaks
R-DRE-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-DRE-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-DRE-5693571 Nonhomologous End-Joining (NHEJ)
R-DRE-5693579 Homologous DNA Pairing and Strand Exchange
R-DRE-5693607 Processing of DNA double-strand break ends
R-DRE-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-DRE-6804756 Regulation of TP53 Activity through Phosphorylation
R-DRE-69473 G2/M DNA damage checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
Nibrin
Alternative name(s):
Nijmegen breakage syndrome protein 1 homolog
Gene namesi
Name:nbn
Synonyms:nbs1
ORF Names:zgc:194152
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi

Organism-specific databases

ZFINiZDB-GENE-041008-35 nbn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002316721 – 818NibrinAdd BLAST818

Proteomic databases

PaxDbiQ5I2W8
PRIDEiQ5I2W8

Expressioni

Gene expression databases

BgeeiENSDARG00000040303
ExpressionAtlasiQ5I2W8 baseline

Interactioni

Subunit structurei

Component of the MRN complex composed of two heterodimers rad50/mre11 associated with a single nbn.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000121705

Structurei

3D structure databases

ProteinModelPortaliQ5I2W8
SMRiQ5I2W8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 70FHAPROSITE-ProRule annotationAdd BLAST49
Domaini91 – 168BRCTAdd BLAST78

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi470 – 475Nuclear localization signalBy similarity6
Motifi800 – 807EEXXXDDL motif8

Domaini

The EEXXXDDL motif at the C-terminus is required for the interaction with atm and its recruitment to sites of DNA damage and promote the phosphorylation of atm substrates, leading to the events of DNA damage response.By similarity

Phylogenomic databases

eggNOGiENOG410IK5M Eukaryota
ENOG410Y1R8 LUCA
GeneTreeiENSGT00390000000521
HOGENOMiHOG000231654
HOVERGENiHBG053070
InParanoidiQ5I2W8
KOiK10867
OMAiYQLSPTK
PhylomeDBiQ5I2W8
TreeFamiTF101103

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00060 FHA, 1 hit
Gene3Di3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR013908 DNA-repair_Nbs1_C
IPR000253 FHA_dom
IPR032429 Nibrin_BRCT2
IPR016592 Nibrin_met
IPR008984 SMAD_FHA_dom_sf
PfamiView protein in Pfam
PF00498 FHA, 1 hit
PF08599 Nbs1_C, 1 hit
PF16508 NIBRIN_BRCT_II, 1 hit
PIRSFiPIRSF011869 Nibrin_animal, 1 hit
SMARTiView protein in SMART
SM00240 FHA, 1 hit
SM01348 Nbs1_C, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
SSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50006 FHA_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q5I2W8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWKLQPTESG GESVILLAGQ EYVVGRKNCE ILLTNDQSIS RVHAVLTVTE
60 70 80 90 100
QAVTLKDSSK YGTFVNGEKL ESGSTKTLQT GYKITFGVFQ SKFSLEKECI
110 120 130 140 150
VVCSSCVDNE GKVTLSQDIR SVGGRLVSSW TSDCTHLVMP TVKVTIKTIC
160 170 180 190 200
ALLCCRPIVK PAFFSALSKA VQQKLPLPKA ERFRPQIDEP SLARDDVDLS
210 220 230 240 250
ARPERKSLFK GKTFLFLSSK QMKRLSVAVS CGGGVSQLLD EGALPVSLLE
260 270 280 290 300
SSSTCVLDMI SGNSQPVISP ASKKWLDSVG QILHRKGLRF ITESEVGLAA
310 320 330 340 350
IHVSNQTYCN PCSSLQSESV KTNPVFASAT LSQSTAVDET ALAAPSQNIT
360 370 380 390 400
AYVVNTEISQ DQSRMVTSGI SAVGETPEKT NPTQKASTTN KPLSLGQEPS
410 420 430 440 450
STRIVQETVM SSESFSVVES EQKMKKGSVV SARGRVEGPV KQKAPSSGNT
460 470 480 490 500
TLKHSPQKQT ALTSFFQPSS KKRPRESSAS SVQPEPKFFK KDIKDNEDDI
510 520 530 540 550
QQSFSVNRSH KTSSEETSLG QACGTGQNSS SKKRKEPEQD TLLGAEEPTA
560 570 580 590 600
ADDLEMSLEE LEFLMSDEMD EPPQTAANKK QRLESGLTSK INSEQLSNQQ
610 620 630 640 650
EVTESKGRKG EKNQQSSSSN IQSMQLDRAG PAVTNQDTQT QSKRSPPDLE
660 670 680 690 700
AHSSANKGPS KNKTPELEEV KKEEVSFVVN SRPQNGISQT SEAVLKQEMQ
710 720 730 740 750
ASTSNSGPKN DPDLPRKLLQ VQFMSLTVNN SSRSRPGPLQ THNPNDKNVK
760 770 780 790 800
RFRKKNVPGF DGLPKIIGGS DLVAHNRSKH SELEEWLRQA AEEEKLNERE
810
ETLGDDLFRY NPRPAKKR
Length:818
Mass (Da):89,407
Last modified:October 14, 2008 - v2
Checksum:iDCF20D4B7A0374E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167L → F in AAW50708 (Ref. 1) Curated1
Sequence conflicti196D → E in AAW50708 (Ref. 1) Curated1
Sequence conflicti200S → G in AAW50708 (Ref. 1) Curated1
Sequence conflicti504F → S in AAW50708 (Ref. 1) Curated1
Sequence conflicti660S → T in AAW50708 (Ref. 1) Curated1
Sequence conflicti700Q → R in AAW50708 (Ref. 1) Curated1
Sequence conflicti802T → Y in AAW50708 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY858826 mRNA Translation: AAW50708.1
BC162723 mRNA Translation: AAI62723.1
RefSeqiNP_001014819.1, NM_001014819.1
UniGeneiDr.83220

Genome annotation databases

EnsembliENSDART00000058974; ENSDARP00000058973; ENSDARG00000040303
GeneIDi544655
KEGGidre:544655

Similar proteinsi

Entry informationi

Entry nameiNBN_DANRE
AccessioniPrimary (citable) accession number: Q5I2W8
Secondary accession number(s): B3DHD3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 14, 2008
Last modified: March 28, 2018
This is version 83 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health