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Protein

Toll-like receptor 9

Gene

TLR9

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Toll-like receptor 9
Alternative name(s):
CD_antigen: CD289
Gene namesi
Name:TLR9
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 815ExtracellularSequence analysisAdd BLAST791
Transmembranei816 – 836HelicalSequence analysisAdd BLAST21
Topological domaini837 – 1029CytoplasmicSequence analysisAdd BLAST193

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000022700625 – 1029Toll-like receptor 9Add BLAST1005

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi63N-linked (GlcNAc...)Sequence analysis1
Glycosylationi128N-linked (GlcNAc...)Sequence analysis1
Glycosylationi199N-linked (GlcNAc...)Sequence analysis1
Glycosylationi209N-linked (GlcNAc...)Sequence analysis1
Glycosylationi241N-linked (GlcNAc...)Sequence analysis1
Glycosylationi339N-linked (GlcNAc...)Sequence analysis1
Glycosylationi380N-linked (GlcNAc...)Sequence analysis1
Glycosylationi472N-linked (GlcNAc...)Sequence analysis1
Glycosylationi511N-linked (GlcNAc...)Sequence analysis1
Glycosylationi565N-linked (GlcNAc...)Sequence analysis1
Glycosylationi667N-linked (GlcNAc...)Sequence analysis1
Glycosylationi692N-linked (GlcNAc...)Sequence analysis1
Glycosylationi729N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5I2M5.
PRIDEiQ5I2M5.

Interactioni

Subunit structurei

Interacts with MYD88 via their respective TIR domains. Interacts with BTK. Interacts (via transmembrane domain) with UNC93B1. Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum.By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-61514N.
STRINGi9913.ENSBTAP00000024223.

Structurei

Secondary structure

11029
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni30 – 33Combined sources4
Beta strandi34 – 36Combined sources3
Beta strandi41 – 43Combined sources3
Helixi61 – 63Combined sources3
Beta strandi66 – 68Combined sources3
Beta strandi90 – 92Combined sources3
Turni100 – 102Combined sources3
Turni115 – 120Combined sources6
Beta strandi126 – 128Combined sources3
Beta strandi146 – 148Combined sources3
Helixi160 – 162Combined sources3
Beta strandi170 – 172Combined sources3
Beta strandi176 – 178Combined sources3
Turni191 – 196Combined sources6
Beta strandi202 – 204Combined sources3
Beta strandi222 – 225Combined sources4
Helixi236 – 239Combined sources4
Beta strandi246 – 249Combined sources4
Helixi276 – 279Combined sources4
Beta strandi287 – 289Combined sources3
Helixi300 – 303Combined sources4
Beta strandi311 – 313Combined sources3
Turni319 – 321Combined sources3
Helixi322 – 325Combined sources4
Turni328 – 331Combined sources4
Beta strandi337 – 339Combined sources3
Helixi357 – 360Combined sources4
Beta strandi367 – 369Combined sources3
Beta strandi375 – 378Combined sources4
Turni380 – 383Combined sources4
Helixi384 – 388Combined sources5
Beta strandi394 – 396Combined sources3
Beta strandi403 – 405Combined sources3
Helixi407 – 412Combined sources6
Beta strandi418 – 420Combined sources3
Beta strandi474 – 476Combined sources3
Helixi487 – 490Combined sources4
Beta strandi498 – 500Combined sources3
Beta strandi523 – 525Combined sources3
Turni536 – 541Combined sources6
Beta strandi547 – 549Combined sources3
Helixi554 – 557Combined sources4
Helixi567 – 571Combined sources5
Beta strandi577 – 579Combined sources3
Beta strandi587 – 589Combined sources3
Beta strandi595 – 597Combined sources3
Beta strandi600 – 602Combined sources3
Helixi608 – 611Combined sources4
Turni615 – 624Combined sources10
Beta strandi630 – 632Combined sources3
Helixi643 – 647Combined sources5
Beta strandi654 – 657Combined sources4
Helixi669 – 673Combined sources5
Beta strandi679 – 681Combined sources3
Beta strandi703 – 705Combined sources3
Beta strandi727 – 729Combined sources3
Helixi740 – 742Combined sources3
Beta strandi752 – 754Combined sources3
Helixi768 – 774Combined sources7
Turni775 – 778Combined sources4
Beta strandi788 – 790Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WPEX-ray2.38A25-815[»]
ProteinModelPortaliQ5I2M5.
SMRiQ5I2M5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati61 – 84LRR 1Add BLAST24
Repeati86 – 109LRR 2Add BLAST24
Repeati121 – 146LRR 3Add BLAST26
Repeati149 – 165LRR 4Add BLAST17
Repeati166 – 189LRR 5Add BLAST24
Repeati197 – 220LRR 6Add BLAST24
Repeati222 – 241LRR 7Add BLAST20
Repeati242 – 267LRR 8Add BLAST26
Repeati282 – 305LRR 9Add BLAST24
Repeati307 – 331LRR 10Add BLAST25
Repeati332 – 355LRR 11Add BLAST24
Repeati362 – 385LRR 12Add BLAST24
Repeati389 – 412LRR 13Add BLAST24
Repeati414 – 439LRR 14Add BLAST26
Repeati469 – 492LRR 15Add BLAST24
Repeati494 – 517LRR 16Add BLAST24
Repeati518 – 541LRR 17Add BLAST24
Repeati543 – 570LRR 18Add BLAST28
Repeati572 – 596LRR 19Add BLAST25
Repeati598 – 620LRR 20Add BLAST23
Repeati625 – 648LRR 21Add BLAST24
Repeati650 – 673LRR 22Add BLAST24
Repeati674 – 697LRR 23Add BLAST24
Repeati699 – 721LRR 24Add BLAST23
Repeati722 – 745LRR 25Add BLAST24
Repeati747 – 770LRR 26Add BLAST24
Domaini864 – 1012TIRPROSITE-ProRule annotationAdd BLAST149

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi854 – 859Poly-Arg6

Sequence similaritiesi

Belongs to the Toll-like receptor family.Curated
Contains 26 LRR (leucine-rich) repeats.Curated
Contains 1 TIR domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4641. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000230468.
HOVERGENiHBG018601.
InParanoidiQ5I2M5.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
3.80.10.10. 4 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027181. TLR9.
[Graphical view]
PANTHERiPTHR24373:SF37. PTHR24373:SF37. 3 hits.
PfamiPF00560. LRR_1. 1 hit.
PF13516. LRR_6. 1 hit.
PF13855. LRR_8. 3 hits.
[Graphical view]
SMARTiSM00369. LRR_TYP. 17 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
SSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 18 hits.
PS50104. TIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5I2M5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPYCAPHPL SLLVQAAALA AALAEGTLPA FLPCELQPHG QVDCNWLFLK
60 70 80 90 100
SVPHFSAGAP RANVTSLSLI SNRIHHLHDS DFVHLSNLRV LNLKWNCPPA
110 120 130 140 150
GLSPMHFPCR MTIEPNTFLA VPTLEELNLS YNGITTVPAL PSSLVSLSLS
160 170 180 190 200
HTSILVLGPT HFTGLHALRF LYMDGNCYYM NPCPRALEVA PGALLGLGNL
210 220 230 240 250
THLSLKYNNL TEVPRRLPPS LDTLLLSYNH IVTLAPEDLA NLTALRVLDV
260 270 280 290 300
GGNCRRCDHA RNPCRECPKN FPKLHPDTFS HLSRLEGLVL KDSSLYKLEK
310 320 330 340 350
DWFRGLGRLQ VLDLSENFLY DYITKTTIFN DLTQLRRLNL SFNYHKKVSF
360 370 380 390 400
AHLHLASSFG SLVSLEKLDM HGIFFRSLTN ITLQSLTRLP KLQSLHLQLN
410 420 430 440 450
FINQAQLSIF GAFPSLLFVD LSDNRISGAA TPAAALGEVD SRVEVWRLPR
460 470 480 490 500
GLAPGPLDAV SSKDFMPSCN LNFTLDLSRN NLVTIQQEMF TRLSRLQCLR
510 520 530 540 550
LSHNSISQAV NGSQFVPLTS LRVLDLSHNK LDLYHGRSFT ELPQLEALDL
560 570 580 590 600
SYNSQPFSMQ GVGHNLSFVA QLPSLRYLSL AHNGIHSRVS QKLSSASLRA
610 620 630 640 650
LDFSGNSLSQ MWAEGDLYLC FFKGLRNLVQ LDLSENHLHT LLPRHLDNLP
660 670 680 690 700
KSLRQLRLRD NNLAFFNWSS LTVLPRLEAL DLAGNQLKAL SNGSLPPGIR
710 720 730 740 750
LQKLDVSSNS IGFVIPGFFV RATRLIELNL SANALKTVDP SWFGSLAGTL
760 770 780 790 800
KILDVSANPL HCACGAAFVD FLLERQEAVP GLSRRVTCGS PGQLQGRSIF
810 820 830 840 850
TQDLRLCLDE TLSLDCFGLS LLMVALGLAV PMLHHLCGWD LWYCFHLCLA
860 870 880 890 900
HLPRRRRQRG EDTLLYDAVV VFDKVQSAVA DWVYNELRVQ LEERRGRRAL
910 920 930 940 950
RLCLEERDWL PGKTLFENLW ASVYSSRKTM FVLDHTDRVS GLLRASFLLA
960 970 980 990 1000
QQRLLEDRKD VVVLVILRPA AYRSRYVRLR QRLCRQSVLL WPHQPSGQGS
1010 1020
FWANLGIALT RDNRHFYNRN FCRGPTTAE
Length:1,029
Mass (Da):115,407
Last modified:February 15, 2005 - v1
Checksum:i02BD73CFE59E1F8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY859726 mRNA. Translation: AAW50954.1.
UniGeneiBt.12810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY859726 mRNA. Translation: AAW50954.1.
UniGeneiBt.12810.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WPEX-ray2.38A25-815[»]
ProteinModelPortaliQ5I2M5.
SMRiQ5I2M5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61514N.
STRINGi9913.ENSBTAP00000024223.

Proteomic databases

PaxDbiQ5I2M5.
PRIDEiQ5I2M5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG4641. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000230468.
HOVERGENiHBG018601.
InParanoidiQ5I2M5.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
3.80.10.10. 4 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027181. TLR9.
[Graphical view]
PANTHERiPTHR24373:SF37. PTHR24373:SF37. 3 hits.
PfamiPF00560. LRR_1. 1 hit.
PF13516. LRR_6. 1 hit.
PF13855. LRR_8. 3 hits.
[Graphical view]
SMARTiSM00369. LRR_TYP. 17 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
SSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 18 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLR9_BOVIN
AccessioniPrimary (citable) accession number: Q5I2M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.