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Protein

Serine protease inhibitor A3G

Gene

Serpina3g

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine and cysteine protease inhibitor. Can inhibit lysosomal papain-like proteases including the cathepsins B, G, H, K, L and V. Ineffective against elastase, granzyme A, granzyme B, or caspases 3, 8 or 9. Inhibition of cytoplasmic cathepsin B following release from the lysosome may protect cells from apoptosis. This may facilitate the survival of progenitor T-cells and the subsequent development of long term memory CD8 T-cells.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei371 – 3722Reactive bondBy similarity

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • apoptotic process Source: UniProtKB-KW
  • response to cytokine Source: MGI
  • response to peptide hormone Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor, Thiol protease inhibitor

Keywords - Biological processi

Adaptive immunity, Apoptosis, Immunity

Protein family/group databases

MEROPSiI04.959.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor A3G
Short name:
Serpin A3G
Alternative name(s):
Serine protease inhibitor 2A
Short name:
Serpin 2A
Gene namesi
Name:Serpina3g
Synonyms:Spi2A
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:105046. Serpina3g.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Serine protease inhibitor A3GPRO_0000094098Add
BLAST

Proteomic databases

MaxQBiQ5I2A0.
PeptideAtlasiQ5I2A0.
PRIDEiQ5I2A0.

PTM databases

iPTMnetiQ5I2A0.
PhosphoSiteiQ5I2A0.

Expressioni

Tissue specificityi

Expressed in bone marrow (particularly hematopoietic stem cells), heart, kidney, liver, lung, skeletal muscle, spleen, testis, thymus and T-cells.3 Publications

Developmental stagei

T-cell specific expression rises during the differentiation of CD8 T-cell progenitors into memory CD8 T-cells.1 Publication

Inductioni

Induction during apoptosis requires NF-kappa-B, a heterodimer of RELA- and NFKB1.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi203441. 1 interaction.
IntActiQ5I2A0. 2 interactions.
MINTiMINT-7714222.

Structurei

3D structure databases

ProteinModelPortaliQ5I2A0.
SMRiQ5I2A0. Positions 37-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni357 – 38226RCLAdd
BLAST

Domaini

The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the serpin reactive site and the protease. The resulting inactive serpin-protease complex is highly stable (By similarity). Variability within the reactive center loop (RCL) sequences of Serpina3 paralogs may determine target protease specificity.By similarity

Sequence similaritiesi

Belongs to the serpin family.Curated

Phylogenomic databases

HOGENOMiHOG000238521.
HOVERGENiHBG005957.
InParanoidiQ5I2A0.
KOiK04525.
PhylomeDBiQ5I2A0.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5I2A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVSPAVFG CPDVTLGRNT AVREVQENVT SVDSLTLVSS NTDFAFSLYR
60 70 80 90 100
KLVLKNPDEN VVFSPFSICT ALALLSLGAK SNTLKEILEG LKFNLTETPE
110 120 130 140 150
PDIHQGFRYL LDLLSQPGNQ VQISTGSALF IEKHLQILAE FKEKARALYQ
160 170 180 190 200
AEAFTADFQQ PLKATKLIND YVSNHTQGKI KELISGLKES TLMVLVNYIY
210 220 230 240 250
FKGKWKNPFD PNDTFKSEFY LDEKRSVIVS MMKTGYLTTP YFRDEELSCT
260 270 280 290 300
VVELKYTGNA SAMFILPDQG RMQQVEASLQ PETLRKWKNS LKPRMIHELR
310 320 330 340 350
LPKFSISTDY SLEHILPELG IREVFSTQAD LSAITGTKDL RVSQVVHKAV
360 370 380 390 400
LDVAETGTEA AAATGMAGVG CCAVFDFLEI FFNRPFLMII SDTKAHIALF
410 420 430 440
MAKVTNPERS MNFPNGEGAS SQRLESKRLC FGDPLCLIGQ
Length:440
Mass (Da):49,021
Last modified:June 21, 2005 - v2
Checksum:i7F449368DC27DD05
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti328 – 3281Q → H in AAW56612 (Ref. 1) Curated
Sequence conflicti356 – 3561T → K in AAW56612 (Ref. 1) Curated
Sequence conflicti411 – 4111M → T in AAW56612 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY862185 mRNA. Translation: AAW56612.1.
BC057144 mRNA. Translation: AAH57144.1.
RefSeqiNP_033277.1. NM_009251.1.
UniGeneiMm.312628.
Mm.483584.
Mm.486434.

Genome annotation databases

GeneIDi20715.
KEGGimmu:20715.
UCSCiuc007oxa.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY862185 mRNA. Translation: AAW56612.1.
BC057144 mRNA. Translation: AAH57144.1.
RefSeqiNP_033277.1. NM_009251.1.
UniGeneiMm.312628.
Mm.483584.
Mm.486434.

3D structure databases

ProteinModelPortaliQ5I2A0.
SMRiQ5I2A0. Positions 37-408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203441. 1 interaction.
IntActiQ5I2A0. 2 interactions.
MINTiMINT-7714222.

Protein family/group databases

MEROPSiI04.959.

PTM databases

iPTMnetiQ5I2A0.
PhosphoSiteiQ5I2A0.

Proteomic databases

MaxQBiQ5I2A0.
PeptideAtlasiQ5I2A0.
PRIDEiQ5I2A0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20715.
KEGGimmu:20715.
UCSCiuc007oxa.2. mouse.

Organism-specific databases

CTDi20715.
MGIiMGI:105046. Serpina3g.

Phylogenomic databases

HOGENOMiHOG000238521.
HOVERGENiHBG005957.
InParanoidiQ5I2A0.
KOiK04525.
PhylomeDBiQ5I2A0.

Miscellaneous databases

PROiQ5I2A0.
SOURCEiSearch...

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPA3G_MOUSE
AccessioniPrimary (citable) accession number: Q5I2A0
Secondary accession number(s): Q6PG99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: September 7, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The single human alpha1-antichymotrypsin gene (SERPINA3) is represented by a cluster of 14 individual murine paralogs.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.