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Protein

RelA-associated inhibitor

Gene

Ppp1r13l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cardiac muscle contraction Source: MGI
  • cardiac right ventricle morphogenesis Source: MGI
  • embryonic camera-type eye development Source: MGI
  • hair cycle Source: MGI
  • multicellular organismal homeostasis Source: MGI
  • multicellular organism growth Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of cell differentiation Source: Ensembl
  • post-embryonic development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • ventricular cardiac muscle tissue development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-6804759. Regulation of TP53 Activity through Association with Co-factors.

Names & Taxonomyi

Protein namesi
Recommended name:
RelA-associated inhibitor
Alternative name(s):
Inhibitor of ASPP protein
Short name:
Protein iASPP
NFkB-interacting protein 1
PPP1R13B-like protein
Gene namesi
Name:Ppp1r13lImported
Synonyms:Nkip11 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:3525053. Ppp1r13l.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Predominantly cytoplasmic but also nuclear.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Ppp1r13l are the cause of the waved 3 phenotype, a recessive mutation characterized by abnormalities of the heart and skin. Affected animals have open eyes at birth and hair abnormalities. They develop focal cardiac necrosis at an early age which progresses to fatal dilated cardiomyopathy. This is due to a 14-bp deletion which gives rise to a truncated protein.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 824824RelA-associated inhibitorPRO_0000066967Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei84 – 841PhosphoserineBy similarity
Modified residuei100 – 1001PhosphoserineBy similarity
Modified residuei102 – 1021PhosphoserineBy similarity
Modified residuei110 – 1101PhosphoserineBy similarity
Modified residuei113 – 1131PhosphoserineCombined sources
Modified residuei119 – 1191PhosphoserineBy similarity
Modified residuei120 – 1201PhosphoserineBy similarity
Modified residuei123 – 1231PhosphothreonineCombined sources
Modified residuei134 – 1341PhosphoserineBy similarity
Modified residuei183 – 1831PhosphoserineCombined sources
Modified residuei187 – 1871PhosphoserineBy similarity
Modified residuei203 – 2031PhosphoserineBy similarity
Modified residuei275 – 2751PhosphothreonineCombined sources
Modified residuei279 – 2791PhosphoserineBy similarity
Modified residuei307 – 3071PhosphothreonineBy similarity
Modified residuei315 – 3151PhosphoserineBy similarity
Modified residuei331 – 3311PhosphoserineBy similarity
Modified residuei338 – 3381PhosphoserineBy similarity
Modified residuei340 – 3401PhosphothreonineBy similarity
Modified residuei522 – 5221PhosphoserineBy similarity
Modified residuei563 – 5631PhosphoserineBy similarity
Modified residuei593 – 5931PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ5I1X5.
MaxQBiQ5I1X5.
PaxDbiQ5I1X5.
PRIDEiQ5I1X5.

PTM databases

iPTMnetiQ5I1X5.
PhosphoSiteiQ5I1X5.

Expressioni

Tissue specificityi

Most abundant in skin with high levels also found in heart, testis and stomach. In E15.5 embryonic heart, expressed at higher levels in atria than ventricles.1 Publication

Gene expression databases

BgeeiQ5I1X5.
ExpressionAtlasiQ5I1X5. baseline and differential.
GenevisibleiQ5I1X5. MM.

Interactioni

Subunit structurei

Interacts with TP63 and TP73 (By similarity). Interacts with RELA NF-kappa-B subunit and with SP1 via its C-terminal part. Interacts with p53/TP53.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ5I1X5. 1 interaction.
STRINGi10090.ENSMUSP00000047839.

Structurei

3D structure databases

ProteinModelPortaliQ5I1X5.
SMRiQ5I1X5. Positions 612-818.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati655 – 68430ANK 1Sequence analysisAdd
BLAST
Repeati688 – 71730ANK 2Sequence analysisAdd
BLAST
Domaini754 – 81663SH3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi54 – 598545Pro-richSequence analysisAdd
BLAST

Domaini

The N-terminal region is required for cytoplasmic localization.By similarity

Sequence similaritiesi

Belongs to the ASPP family.Sequence analysis
Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG0515. Eukaryota.
ENOG410Y17W. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000112990.
HOVERGENiHBG055210.
InParanoidiQ5I1X5.
OMAiMGLMHNG.
OrthoDBiEOG7WDN20.
PhylomeDBiQ5I1X5.
TreeFamiTF105545.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR028320. iASPP.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR24164. PTHR24164. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00248. ANK. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5I1X5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSEAFQHAR DLLDLNFQSL AMKHMDLKQM ELDTAAAKVD ELTKQLESLW
60 70 80 90 100
SDSPAPPGAQ AGVPSRMARY STSPVPEHFG SRGSPQKIAT DGIEARFGRS
110 120 130 140 150
ESAPSLHPYS PLSPKGRPSS PRTPIYLQPD TYSSLDRAPS PRPRAFDGAG
160 170 180 190 200
SPHGRAPSPR PGIGPVRQPG PSTPFDYLGR AGSPRGSPLA EGPQAFFPER
210 220 230 240 250
GPSPRPPAAA YDTAGTFGSP LLGAGGSAFT PPLRAQDDST LRRRPPKAWN
260 270 280 290 300
ESDLDVAYEK KSSQTASYER LDVFTRPASP GLQLLPWRES SLDGLGASGK
310 320 330 340 350
DHLTSATLPR NYKVSPLASD RRSDVGSYRR SLGSAGPSGT LPRSWQPVSR
360 370 380 390 400
IPMPPSSPQP RSTPRQRPIP LSMIFKLQNA FWEHGAGRAV LPGSPIFSRA
410 420 430 440 450
PPPKLPPQPP PQPQMQPQPQ PQPQMQPQSQ AQPQTPAPQQ TWSPMNEGLL
460 470 480 490 500
KSPAELEPEP ELEVLLAPVE EAGDADEGTV TRPLSPTRLQ PALPPEAQTV
510 520 530 540 550
PELEEVARVL AEIPRPLKRR GSMEQSPAVA LPPTHKKQYQ QIINRLFHRH
560 570 580 590 600
GGPGPGGPEP ELSTITEGSE ARAGPPAPAP PAPIPPPAPP QSSPPEQPQS
610 620 630 640 650
MEMRSVLRKV GSPRKARRAR LNPLVLLLDA ALTGELDVVQ QAVKEMNDPS
660 670 680 690 700
QPNEEGITAL HNAICGANYP IVDFLIAAGA NVNSPDSHGW TPLHCAASCN
710 720 730 740 750
DTAICTALVQ HGAAIFATTL SDGATAIEKC DPYREGYADC ATYLADVEQS
760 770 780 790 800
MGLMHNGVVY ALWDYSAEFG DELSFREGES VTVLRRDGPE ETDWWWASLH
810 820
GQEGYVPRNY FGLFPRVKSQ RSKI
Length:824
Mass (Da):88,976
Last modified:February 15, 2005 - v1
Checksum:i57AC88589BC8AA8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY869711 mRNA. Translation: AAW51145.1.
CCDSiCCDS20899.1.
RefSeqiNP_001010836.1. NM_001010836.3.
XP_006540189.1. XM_006540126.1.
XP_006540190.1. XM_006540127.2.
XP_006540191.1. XM_006540128.1.
XP_006540192.1. XM_006540129.1.
XP_011248911.1. XM_011250609.1.
UniGeneiMm.333640.

Genome annotation databases

EnsembliENSMUST00000047621; ENSMUSP00000047839; ENSMUSG00000040734.
GeneIDi333654.
KEGGimmu:333654.
UCSCiuc009flo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY869711 mRNA. Translation: AAW51145.1.
CCDSiCCDS20899.1.
RefSeqiNP_001010836.1. NM_001010836.3.
XP_006540189.1. XM_006540126.1.
XP_006540190.1. XM_006540127.2.
XP_006540191.1. XM_006540128.1.
XP_006540192.1. XM_006540129.1.
XP_011248911.1. XM_011250609.1.
UniGeneiMm.333640.

3D structure databases

ProteinModelPortaliQ5I1X5.
SMRiQ5I1X5. Positions 612-818.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5I1X5. 1 interaction.
STRINGi10090.ENSMUSP00000047839.

PTM databases

iPTMnetiQ5I1X5.
PhosphoSiteiQ5I1X5.

Proteomic databases

EPDiQ5I1X5.
MaxQBiQ5I1X5.
PaxDbiQ5I1X5.
PRIDEiQ5I1X5.

Protocols and materials databases

DNASUi333654.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047621; ENSMUSP00000047839; ENSMUSG00000040734.
GeneIDi333654.
KEGGimmu:333654.
UCSCiuc009flo.1. mouse.

Organism-specific databases

CTDi10848.
MGIiMGI:3525053. Ppp1r13l.

Phylogenomic databases

eggNOGiKOG0515. Eukaryota.
ENOG410Y17W. LUCA.
GeneTreeiENSGT00760000119141.
HOGENOMiHOG000112990.
HOVERGENiHBG055210.
InParanoidiQ5I1X5.
OMAiMGLMHNG.
OrthoDBiEOG7WDN20.
PhylomeDBiQ5I1X5.
TreeFamiTF105545.

Enzyme and pathway databases

ReactomeiR-MMU-6804759. Regulation of TP53 Activity through Association with Co-factors.

Miscellaneous databases

ChiTaRSiPpp1r13l. mouse.
PROiQ5I1X5.
SOURCEiSearch...

Gene expression databases

BgeeiQ5I1X5.
ExpressionAtlasiQ5I1X5. baseline and differential.
GenevisibleiQ5I1X5. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR028320. iASPP.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR24164. PTHR24164. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00248. ANK. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A mutation in NFkB interacting protein 1 results in cardiomyopathy and abnormal skin development in wa3 mice."
    Herron B.J., Rao C., Liu S., Laprade L., Richardson J.A., Olivieri E., Semsarian C., Millar S.E., Stubbs L., Beier D.R.
    Hum. Mol. Genet. 14:667-677(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DISEASE, INTERACTION WITH RELA.
    Strain: DBA/2JImported.
  2. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-113; THR-123; SER-183 AND THR-275, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart and Testis.

Entry informationi

Entry nameiIASPP_MOUSE
AccessioniPrimary (citable) accession number: Q5I1X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: February 15, 2005
Last modified: July 6, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.