Q5I146 (PTPH2_MDBVW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine phosphatase H2
Short name=PTP-H2
EC=3.1.3.48

Gene names

Name:

H2

Organism

Microplitis demolitor bracovirus (isolate Webb) (MdBV) [Reference proteome]

Taxonomic identifier

654919 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Polydnaviridae › Bracovirus › Microplitis demolitor bracovirus

Virus host

Microplitis demolitor [TaxID: 69319]

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Suppresses host immune cell adhesion and phagocytosis. Triggers host mitochondrial membrane depolarization and caspase-dependent apoptosis. Ref.2 Ref.3
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Host cytoplasm Ref.4.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Biological process	Activation of host caspases by virus Host-virus interaction Inhibition of host innate immune response by virus Modulation of host cell apoptosis by virus Viral immunoevasion
Cellular component	Host cytoplasm
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	induction by virus of host cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC suppression by virus of host innate immune response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Tyrosine phosphatase H2		PRO_0000405352
Regions
	Domain	27 – 295	269	Tyrosine-protein phosphatase
Sites
	Active site	236	1	Phosphocysteine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5I146 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: D83B669F822005CF	FASTA	325	37,876
	10 20 30 40 50 60 MSRCKFRSLN YSEFLNLVEK SDFEEVVTRE HEKIMAQKVD GTFNESMKLE NRKLNRYLDM 70 80 90 100 110 120 LCFDHTRVTL PAEKNRGDYI NANYVDGYEY KKKFICTQAP LQQTAYDFWR TVWMHHTRII 130 140 150 160 170 180 VMMCKKKENR KQCFAYWNDI EGGDIVFGKF KITTTQIETH LSYIETTLLV TDGTSAIQEV 190 200 210 220 230 240 THFVFTQWPD YGVPNDVMNL LNFILTVKSA QKDVIRQLAQ ERFKIGDNPP PIVVHCSAGV 250 260 270 280 290 300 GRTGAYCLLD SAISEFDACA TISIPSTLIN IRNQRYYCIF ILPQYFFCYR VMERYVNLTV 310 320 NKVSKKLIAN VATALFNKVL HLKDN

References

[1]	"Polydnavirus genomes reflect their dual roles as mutualists and pathogens." Webb B.A., Strand M.R., Dickey S.E., Beck M.H., Hilgarth R.S., Barney W.E., Kadash K., Kroemer J.A., Lindstrom K.G., Rattanadechakul W., Shelby K.S., Thoetkiattikul H., Turnbull M.W., Witherell R.A. Virology 347:160-174(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"PTP-H2 and PTP-H3 from Microplitis demolitor Bracovirus localize to focal adhesions and are antiphagocytic in insect immune cells." Pruijssers A.J., Strand M.R. J. Virol. 81:1209-1219(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"Protein tyrosine phosphatase-H2 from a polydnavirus induces apoptosis of insect cells." Suderman R.J., Pruijssers A.J., Strand M.R. J. Gen. Virol. 89:1411-1420(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Characterization and kinetic analysis of protein tyrosine phosphatase-H2 from Microplitis demolitor bracovirus." Eum J.H., Bottjen R.C., Pruijssers A.J., Clark K.D., Strand M.R. Insect Biochem. Mol. Biol. 40:690-698(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY875685 Genomic DNA. Translation: AAW51786.1.
RefSeq	YP_239382.1. NC_007035.1.
3D structure databases
ProteinModelPortal	Q5I146.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5075819.
Phylogenomic databases
ProtClustDB	PHA2738.
Family and domain databases
InterPro	IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTPH2_MDBVW

Accession

Primary (citable) accession number: Q5I146

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 8, 2011
Last sequence update:	February 15, 2005
Last modified:	March 6, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program