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Protein

Glycine cleavage system H protein, mitochondrial

Gene

Gcsh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The H protein (GCSH) shuttles the methylamine group of glycine from the P protein (GLDC) to the T protein (GCST) (By similarity).By similarity

Cofactori

(R)-lipoateBy similarityNote: Binds 1 lipoyl cofactor covalently.By similarity

GO - Molecular functioni

  • aminomethyltransferase activity Source: RGD
  • enzyme binding Source: RGD

GO - Biological processi

  • glycine decarboxylation via glycine cleavage system Source: RGD
  • methylation Source: GOC
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-389661. Glyoxylate metabolism and glycine degradation.
R-RNO-6783984. Glycine degradation.
SABIO-RKQ5I0P2.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine cleavage system H protein, mitochondrial
Alternative name(s):
Lipoic acid-containing protein
Gene namesi
Name:Gcsh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi619946. Gcsh.

Subcellular locationi

GO - Cellular componenti

  • glycine cleavage complex Source: RGD
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4545MitochondrionBy similarityAdd
BLAST
Chaini46 – 170125Glycine cleavage system H protein, mitochondrialPRO_0000010726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei104 – 1041N6-lipoyllysinePROSITE-ProRule annotationBy similarity

Proteomic databases

PaxDbiQ5I0P2.
PRIDEiQ5I0P2.

PTM databases

iPTMnetiQ5I0P2.
PhosphoSiteiQ5I0P2.

Expressioni

Gene expression databases

GenevisibleiQ5I0P2. RN.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P (GLDC), T (GCST), L (DLD) and H (GCSH). Interacts with GLDC (By similarity).By similarity

GO - Molecular functioni

  • enzyme binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015967.

Structurei

3D structure databases

ProteinModelPortaliQ5I0P2.
SMRiQ5I0P2. Positions 41-170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 14583Lipoyl-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GcvH family.Curated
Contains 1 lipoyl-binding domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

eggNOGiKOG3373. Eukaryota.
COG0509. LUCA.
GeneTreeiENSGT00390000011666.
HOGENOMiHOG000239392.
HOVERGENiHBG001129.
InParanoidiQ5I0P2.
KOiK02437.
OMAiNTDPYGE.
OrthoDBiEOG7RNK1M.
PhylomeDBiQ5I0P2.
TreeFamiTF300258.

Family and domain databases

HAMAPiMF_00272. GcvH.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR002930. GCV_H.
IPR017453. GCV_H_sub.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR11715. PTHR11715. 1 hit.
PfamiPF01597. GCV_H. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
TIGRFAMsiTIGR00527. gcvH. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5I0P2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRVVRSVR AVACSLRIAL ASCPPRPWAP SAAAVRSLRT GSALLSVRKF
60 70 80 90 100
TEKHEWVTAK DGIGTVGISN FAQEALGDVV YCSLPEVGTK LKKQEEFGAL
110 120 130 140 150
ESVKAASELY SPLSGEVTEV NEALAENPGL VNKSCYEDGW LIKMTLSDPS
160 170
ELDELMSEEA YEKYVKSIEE
Length:170
Mass (Da):18,485
Last modified:February 15, 2005 - v1
Checksum:i6E4CD58F28CD26A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088114 mRNA. Translation: AAH88114.1.
RefSeqiNP_598282.2. NM_133598.2.
UniGeneiRn.98456.

Genome annotation databases

EnsembliENSRNOT00000015967; ENSRNOP00000015967; ENSRNOG00000011535.
GeneIDi171133.
KEGGirno:171133.
UCSCiRGD:619946. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088114 mRNA. Translation: AAH88114.1.
RefSeqiNP_598282.2. NM_133598.2.
UniGeneiRn.98456.

3D structure databases

ProteinModelPortaliQ5I0P2.
SMRiQ5I0P2. Positions 41-170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015967.

PTM databases

iPTMnetiQ5I0P2.
PhosphoSiteiQ5I0P2.

Proteomic databases

PaxDbiQ5I0P2.
PRIDEiQ5I0P2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015967; ENSRNOP00000015967; ENSRNOG00000011535.
GeneIDi171133.
KEGGirno:171133.
UCSCiRGD:619946. rat.

Organism-specific databases

CTDi2653.
RGDi619946. Gcsh.

Phylogenomic databases

eggNOGiKOG3373. Eukaryota.
COG0509. LUCA.
GeneTreeiENSGT00390000011666.
HOGENOMiHOG000239392.
HOVERGENiHBG001129.
InParanoidiQ5I0P2.
KOiK02437.
OMAiNTDPYGE.
OrthoDBiEOG7RNK1M.
PhylomeDBiQ5I0P2.
TreeFamiTF300258.

Enzyme and pathway databases

ReactomeiR-RNO-389661. Glyoxylate metabolism and glycine degradation.
R-RNO-6783984. Glycine degradation.
SABIO-RKQ5I0P2.

Miscellaneous databases

NextBioi621902.
PROiQ5I0P2.

Gene expression databases

GenevisibleiQ5I0P2. RN.

Family and domain databases

HAMAPiMF_00272. GcvH.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR002930. GCV_H.
IPR017453. GCV_H_sub.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR11715. PTHR11715. 1 hit.
PfamiPF01597. GCV_H. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
TIGRFAMsiTIGR00527. gcvH. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiGCSH_RAT
AccessioniPrimary (citable) accession number: Q5I0P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: February 15, 2005
Last modified: February 17, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.