Q5I0P2 (GCSH_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine cleavage system H protein, mitochondrial

Gene names

Name:

Gcsh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein By similarity.
Cofactor	Binds 1 lipoyl cofactor covalently By similarity.
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the GcvH family. Contains 1 lipoyl-binding domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Lipoyl Transit peptide
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from mutant phenotype. Source: RGD
Cellular component	glycine cleavage complex Inferred by curator. Source: RGD mitochondrion Inferred from direct assay. Source: RGD
Molecular function	aminomethyltransferase activity Inferred from mutant phenotype. Source: RGD enzyme binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 45	45	Mitochondrion By similarity
	Chain	46 – 170	125	Glycine cleavage system H protein, mitochondrial		PRO_0000010726
Amino acid modifications
	Modified residue	104	1	N6-lipoyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5I0P2 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 6E4CD58F28CD26A5	FASTA	170	18,485
	10 20 30 40 50 60 MSLRVVRSVR AVACSLRIAL ASCPPRPWAP SAAAVRSLRT GSALLSVRKF TEKHEWVTAK 70 80 90 100 110 120 DGIGTVGISN FAQEALGDVV YCSLPEVGTK LKKQEEFGAL ESVKAASELY SPLSGEVTEV 130 140 150 160 170 NEALAENPGL VNKSCYEDGW LIKMTLSDPS ELDELMSEEA YEKYVKSIEE

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC088114 mRNA. Translation: AAH88114.1.
IPI	IPI00214454.
RefSeq	NP_598282.2. NM_133598.2.
UniGene	Rn.98456.
3D structure databases
ProteinModelPortal	Q5I0P2.
SMR	Q5I0P2. Positions 41-170.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5I0P2.
PTM databases
PhosphoSite	Q5I0P2.
Proteomic databases
PRIDE	Q5I0P2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000015967; ENSRNOP00000015967; ENSRNOG00000011535.
GeneID	171133.
KEGG	rno:171133.
NMPDR	fig|10116.3.peg.15083.
UCSC	BC088114. rat.
Organism-specific databases
CTD	2653.
RGD	619946. Gcsh.
Phylogenomic databases
eggNOG	roNOG07568.
GeneTree	ENSGT00390000011666.
HOVERGEN	HBG001129.
InParanoid	Q5I0P2.
OMA	ALVNSSC.
OrthoDB	EOG43N7DX.
PhylomeDB	Q5I0P2.
Gene expression databases
ArrayExpress	Q5I0P2.
Genevestigator	Q5I0P2.
GermOnline	ENSRNOG00000011535. Rattus norvegicus.
Family and domain databases
InterPro	IPR003016. 2-oxoA_DH_lipoyl-BS. IPR002930. GCV_H. IPR017453. GCV_H_sub. IPR011053. Single_hybrid_motif. [Graphical view]
KO	K02437.
PANTHER	PTHR11715. GCV_H. 1 hit.
Pfam	PF01597. GCV_H. 1 hit. [Graphical view]
SUPFAM	SSF51230. Hybrid_motif. 1 hit.
TIGRFAMs	TIGR00527. GcvH. 1 hit.
PROSITE	PS00189. LIPOYL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	621902.

Entry information

Entry name

GCSH_RAT

Accession

Primary (citable) accession number: Q5I0P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	February 15, 2005
Last modified:	November 16, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program