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Protein

Nicotinate-nucleotide pyrophosphorylase [carboxylating]

Gene

Qprt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the catabolism of quinolinic acid (QA).By similarity

Catalytic activityi

Beta-nicotinate D-ribonucleotide + diphosphate + CO2 = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate.
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate-nucleotide pyrophosphorylase [carboxylating] (Qprt)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021SubstrateBy similarity
Binding sitei161 – 1611SubstrateBy similarity
Binding sitei171 – 1711SubstrateBy similarity
Binding sitei201 – 2011SubstrateBy similarity
Binding sitei222 – 2221SubstrateBy similarity

GO - Molecular functioni

  • nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: RGD

GO - Biological processi

  • NAD biosynthetic process Source: RGD
  • quinolinate catabolic process Source: GO_Central
  • quinolinate metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Enzyme and pathway databases

ReactomeiR-RNO-196807. Nicotinate metabolism.
UniPathwayiUPA00253; UER00331.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC:2.4.2.19)
Alternative name(s):
Quinolinate phosphoribosyltransferase [decarboxylating]
Short name:
QAPRTase
Short name:
QPRTase
Gene namesi
Name:Qprt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1310309. Qprt.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Nicotinate-nucleotide pyrophosphorylase [carboxylating]PRO_0000245464Add
BLAST

Proteomic databases

PaxDbiQ5I0M2.
PRIDEiQ5I0M2.

PTM databases

iPTMnetiQ5I0M2.

Expressioni

Gene expression databases

GenevisibleiQ5I0M2. RN.

Interactioni

Subunit structurei

Hexamer formed by 3 homodimers.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023080.

Structurei

3D structure databases

ProteinModelPortaliQ5I0M2.
SMRiQ5I0M2. Positions 1-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1393Substrate bindingBy similarity
Regioni248 – 2503Substrate bindingBy similarity
Regioni269 – 2713Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the NadC/ModD family.Curated

Phylogenomic databases

eggNOGiKOG3008. Eukaryota.
COG0157. LUCA.
GeneTreeiENSGT00390000002761.
HOGENOMiHOG000224023.
HOVERGENiHBG031727.
InParanoidiQ5I0M2.
KOiK00767.
OMAiLYDMIML.
OrthoDBiEOG77Q4X9.
PhylomeDBiQ5I0M2.
TreeFamiTF300845.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]
PfamiPF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5I0M2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPEGLQFLL PPASLAALAN SWLQEDCPGL NFASLVTGSA PAQAVLWAKS
60 70 80 90 100
PGVLAGRPFF DAIFTQLNCQ VSWLLPEGSK LVPVVKVAEV RGPAHHLLLG
110 120 130 140 150
ERVALNTLAR CSGIASAAAT AVEVATSTGW AGHVAGTRKT TPGFRLVEKY
160 170 180 190 200
GLLVGGAECH RYDLGGLVMV KDNHVVAAGS MEKAVLKARQ AAGFPLKVEV
210 220 230 240 250
ECSSLKEALQ AAEAGADLVM LDNFKPEELH PTAATLKAKF PNVSVEASGG
260 270 280 290
VTLDNLPQFC GTNIDVISLG MLTQAAPALD FSLKLYAEGD IPVPHARRS
Length:299
Mass (Da):31,297
Last modified:February 15, 2005 - v1
Checksum:i7ADC91705C422FD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088177 mRNA. Translation: AAH88177.1.
RefSeqiNP_001009646.1. NM_001009646.1.
UniGeneiRn.9220.

Genome annotation databases

EnsembliENSRNOT00000023080; ENSRNOP00000023080; ENSRNOG00000016980.
GeneIDi293504.
KEGGirno:293504.
UCSCiRGD:1310309. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088177 mRNA. Translation: AAH88177.1.
RefSeqiNP_001009646.1. NM_001009646.1.
UniGeneiRn.9220.

3D structure databases

ProteinModelPortaliQ5I0M2.
SMRiQ5I0M2. Positions 1-288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023080.

PTM databases

iPTMnetiQ5I0M2.

Proteomic databases

PaxDbiQ5I0M2.
PRIDEiQ5I0M2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023080; ENSRNOP00000023080; ENSRNOG00000016980.
GeneIDi293504.
KEGGirno:293504.
UCSCiRGD:1310309. rat.

Organism-specific databases

CTDi23475.
RGDi1310309. Qprt.

Phylogenomic databases

eggNOGiKOG3008. Eukaryota.
COG0157. LUCA.
GeneTreeiENSGT00390000002761.
HOGENOMiHOG000224023.
HOVERGENiHBG031727.
InParanoidiQ5I0M2.
KOiK00767.
OMAiLYDMIML.
OrthoDBiEOG77Q4X9.
PhylomeDBiQ5I0M2.
TreeFamiTF300845.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00331.
ReactomeiR-RNO-196807. Nicotinate metabolism.

Miscellaneous databases

PROiQ5I0M2.

Gene expression databases

GenevisibleiQ5I0M2. RN.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]
PfamiPF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiNADC_RAT
AccessioniPrimary (citable) accession number: Q5I0M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: February 15, 2005
Last modified: July 6, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.