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Protein

Cytosolic Fe-S cluster assembly factor NUBP1

Gene

Nubp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Implicated in the regulation of centrosome duplication. Negatively regulates cilium formation and structure.UniRule annotationBy similarity

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi22Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi25Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi31Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi235Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi238Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 69ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP1UniRule annotation
Alternative name(s):
Nucleotide-binding protein 1UniRule annotation
Short name:
NBP 1UniRule annotation
Gene namesi
Name:Nubp1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1310514. Nubp1.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus By similarity
  • Cell projection By similarity
  • Cytoplasmcytoskeletoncilium axoneme By similarity
  • Cytoplasmcytoskeletoncilium basal body By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing center By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity

  • Note: Enriched in centrioles of microtubule asters during prophase, prometaphase and telophase stages of mitosis. Localized at centrioles and in the nucleus at interphase. Colocalizes with nubp-2 at prometaphase. Specifically localizes to the axenome of motile cilia as opposed to primary non-motile cilia. Localization is independent of NUBP2 and KIFC1.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003061701 – 320Cytosolic Fe-S cluster assembly factor NUBP1Add BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei319PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5I0L4.
PRIDEiQ5I0L4.

PTM databases

iPTMnetiQ5I0L4.
PhosphoSitePlusiQ5I0L4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000002574.
GenevisibleiQ5I0L4. RN.

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1. Interacts with the BBS/CCT complex subunit CCT1.UniRule annotationBy similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003479.

Structurei

3D structure databases

ProteinModelPortaliQ5I0L4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
GeneTreeiENSGT00390000009735.
HOGENOMiHOG000079916.
HOVERGENiHBG051027.
InParanoidiQ5I0L4.
OMAiQRIREFC.
OrthoDBiEOG091G0DY5.
PhylomeDBiQ5I0L4.
TreeFamiTF300755.

Family and domain databases

CDDicd02037. MRP-like. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03038. NUBP1. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
IPR033756. YlxH/NBP35.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5I0L4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGAPHGCPG ADSAQAGRGA SCQGCPNQKL CASGAGAAPD PAVEEIREKM
60 70 80 90 100
KTVRHRILVL SGKGGVGKST FSAHLAHGLA EDGDTQVALL DIDICGPSIP
110 120 130 140 150
KIMGLEGEQV HQSGSGWSPV YVEDNLGVMS VGFLLSSPDD AVIWRGPKKN
160 170 180 190 200
GMIKQFLRDV DWGDVDYLVI DTPPGTSDEH LSVVQYLAAA HIDGAVILTT
210 220 230 240 250
PQEVALQDVR KEISFCHKVK LPIIGVVENM SGFICPKCKR ESQIFPPTTG
260 270 280 290 300
GAEAMCQALK IPLLGKVPLD PHIGKSCDKG QSFFVEAPDS PATAAYKSII
310 320
QRIREFCNSR QSHDENLISP
Length:320
Mass (Da):34,045
Last modified:February 15, 2005 - v1
Checksum:i3C9981B055278BD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088221 mRNA. Translation: AAH88221.1.
RefSeqiNP_001009619.1. NM_001009619.1.
UniGeneiRn.1758.

Genome annotation databases

EnsembliENSRNOT00000003479; ENSRNOP00000003479; ENSRNOG00000002574.
GeneIDi287042.
KEGGirno:287042.
UCSCiRGD:1310514. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088221 mRNA. Translation: AAH88221.1.
RefSeqiNP_001009619.1. NM_001009619.1.
UniGeneiRn.1758.

3D structure databases

ProteinModelPortaliQ5I0L4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003479.

PTM databases

iPTMnetiQ5I0L4.
PhosphoSitePlusiQ5I0L4.

Proteomic databases

PaxDbiQ5I0L4.
PRIDEiQ5I0L4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003479; ENSRNOP00000003479; ENSRNOG00000002574.
GeneIDi287042.
KEGGirno:287042.
UCSCiRGD:1310514. rat.

Organism-specific databases

CTDi4682.
RGDi1310514. Nubp1.

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
GeneTreeiENSGT00390000009735.
HOGENOMiHOG000079916.
HOVERGENiHBG051027.
InParanoidiQ5I0L4.
OMAiQRIREFC.
OrthoDBiEOG091G0DY5.
PhylomeDBiQ5I0L4.
TreeFamiTF300755.

Miscellaneous databases

PROiQ5I0L4.

Gene expression databases

BgeeiENSRNOG00000002574.
GenevisibleiQ5I0L4. RN.

Family and domain databases

CDDicd02037. MRP-like. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03038. NUBP1. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
IPR033756. YlxH/NBP35.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUBP1_RAT
AccessioniPrimary (citable) accession number: Q5I0L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: February 15, 2005
Last modified: November 30, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.