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Protein

Cytosolic Fe-S cluster assembly factor NUBP1

Gene

Nubp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Implicated in the regulation of centrosome duplication. Negatively regulates cilium formation and structure.UniRule annotationBy similarity

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi22Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi25Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi31Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi235Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi238Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 69ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCilium biogenesis/degradation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP1UniRule annotation
Alternative name(s):
Nucleotide-binding protein 1UniRule annotation
Short name:
NBP 1UniRule annotation
Gene namesi
Name:Nubp1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1310514 Nubp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003061701 – 320Cytosolic Fe-S cluster assembly factor NUBP1Add BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei319PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5I0L4
PRIDEiQ5I0L4

PTM databases

iPTMnetiQ5I0L4
PhosphoSitePlusiQ5I0L4

Expressioni

Gene expression databases

BgeeiENSRNOG00000002574
GenevisibleiQ5I0L4 RN

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1. Interacts with the BBS/CCT complex subunit CCT1.UniRule annotationBy similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003479

Structurei

3D structure databases

ProteinModelPortaliQ5I0L4
SMRiQ5I0L4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ Eukaryota
COG0489 LUCA
GeneTreeiENSGT00390000009735
HOGENOMiHOG000079916
HOVERGENiHBG051027
InParanoidiQ5I0L4
OMAiCPNQAIC
OrthoDBiEOG091G0DY5
PhylomeDBiQ5I0L4
TreeFamiTF300755

Family and domain databases

CDDicd02037 MRP-like, 1 hit
HAMAPiMF_02040 Mrp_NBP35, 1 hit
MF_03038 NUBP1, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR019591 Mrp/NBP35_ATP-bd
IPR000808 Mrp_CS
IPR028601 NUBP1/Nbp35
IPR027417 P-loop_NTPase
IPR033756 YlxH/NBP35
PANTHERiPTHR23264 PTHR23264, 1 hit
PfamiView protein in Pfam
PF10609 ParA, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS01215 MRP, 1 hit

Sequencei

Sequence statusi: Complete.

Q5I0L4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGAPHGCPG ADSAQAGRGA SCQGCPNQKL CASGAGAAPD PAVEEIREKM
60 70 80 90 100
KTVRHRILVL SGKGGVGKST FSAHLAHGLA EDGDTQVALL DIDICGPSIP
110 120 130 140 150
KIMGLEGEQV HQSGSGWSPV YVEDNLGVMS VGFLLSSPDD AVIWRGPKKN
160 170 180 190 200
GMIKQFLRDV DWGDVDYLVI DTPPGTSDEH LSVVQYLAAA HIDGAVILTT
210 220 230 240 250
PQEVALQDVR KEISFCHKVK LPIIGVVENM SGFICPKCKR ESQIFPPTTG
260 270 280 290 300
GAEAMCQALK IPLLGKVPLD PHIGKSCDKG QSFFVEAPDS PATAAYKSII
310 320
QRIREFCNSR QSHDENLISP
Length:320
Mass (Da):34,045
Last modified:February 15, 2005 - v1
Checksum:i3C9981B055278BD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088221 mRNA Translation: AAH88221.1
RefSeqiNP_001009619.1, NM_001009619.1
UniGeneiRn.1758

Genome annotation databases

EnsembliENSRNOT00000003479; ENSRNOP00000003479; ENSRNOG00000002574
GeneIDi287042
KEGGirno:287042
UCSCiRGD:1310514 rat

Similar proteinsi

Entry informationi

Entry nameiNUBP1_RAT
AccessioniPrimary (citable) accession number: Q5I0L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: February 15, 2005
Last modified: April 25, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health