Q5I0L3 (SYYM_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase, mitochondrial
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:

Yars2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 471		Tyrosine--tRNA ligase, mitochondrial		PRO_0000250721
Regions
	Motif	76 – 85	10	"HIGH" region
	Motif	275 – 279	5	"KMSKS" region
Sites
	Binding site	278	1	ATP By similarity
Amino acid modifications
	Modified residue	349	1	N6-acetyllysine By similarity
	Modified residue	420	1	Phosphotyrosine By similarity
	Modified residue	429	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5I0L3 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: A9431FD9EA3FBE92	FASTA	471	52,628
	10 20 30 40 50 60 MAAPMLRHLC RVPQSGVWTR GPRAVRPGAR GMLVAPRARG LFKEFFPESG TKTELPELFD 70 80 90 100 110 120 RRRAGSPQTV YCGFDPTGDS LHVGHLLTLL GLFHFQRAGH NVIALVGGST ALLGDPSGRT 130 140 150 160 170 180 KEREALSAEC VRANARALQR GLETLAANHA RLFADGRPWG TFTVLDNAAW FQKQHLMDFL 190 200 210 220 230 240 ATVGGHFRMG TLLSRLSVQS RLKSPEGMSL AEFFYQVLQA YDFYYLFRHY GCRVQLGGSD 250 260 270 280 290 300 QLGNIMSGYE FIHKLTGEDV FGITVPLITS TTGAKLGKSA GNAVWLNREK TSPFELYQFF 310 320 330 340 350 360 IRQQDDSVER YLKLFTFLPL PEIDHIMQLH VKEPEKRIAQ KRLAAEVTKL VHGQEGLDSA 370 380 390 400 410 420 KRCTQALYHS SIEALEVMSD QELKELFKEA SFSELVLDPG TSVIDTCRKA NAIPDGPRGY 430 440 450 460 470 RMITEGGVSI NHRQVTNPES VLVIGQHILK NGLSLLKIGK RNFYIIKWLQ L

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC088224 mRNA. Translation: AAH88224.1.
IPI	IPI00370436.
RefSeq	NP_001009627.1. NM_001009627.1.
UniGene	Rn.163187.
3D structure databases
HSSP	HSSP built from PDB template 4TS1 based on UniProtKB P00952.
ProteinModelPortal	Q5I0L3.
SMR	Q5I0L3. Positions 31-367.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5I0L3.
Proteomic databases
PRIDE	Q5I0L3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000034902; ENSRNOP00000037398; ENSRNOG00000025252.
GeneID	287924.
KEGG	rno:287924.
NMPDR	fig|10116.3.peg.7839.
UCSC	NM_001009627. rat.
Organism-specific databases
CTD	51067.
RGD	1311696. Yars2.
Phylogenomic databases
eggNOG	roNOG05666.
GeneTree	ENSGT00390000013709.
HOVERGEN	HBG056052.
InParanoid	Q5I0L3.
OMA	LVHGQEG.
OrthoDB	EOG47H5Q1.
PhylomeDB	Q5I0L3.
Gene expression databases
ArrayExpress	Q5I0L3.
Genevestigator	Q5I0L3.
GermOnline	ENSRNOG00000025252. Rattus norvegicus.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	627220.

Entry information

Entry name

SYYM_RAT

Accession

Primary (citable) accession number: Q5I0L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program