Reviewed, UniProtKB/Swiss-Prot Q5I0J9 (ASPD_RAT)

Names and origin

Protein names	Recommended name:     Putative L-aspartate dehydrogenase     EC=1.4.1.21
Organism	Rattus norvegicus (Rat)
Taxonomic identifier	10116 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity.
Catalytic activity	L-aspartate + H(2)O + NAD(P)(+) = oxaloacetate + NH(3) + NAD(P)H.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1.
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartate dehydrogenase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Putative L-aspartate dehydrogenase		PRO_0000144902
Sites
	Binding site	131	1	NAD; via amide nitrogen By similarity
	Binding site	196	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5I0J9-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 572A02B436161734	FASTA	297	31,260
	10 20 30 40 50 60 MDASMVPRVP HKVGVVGYGR LGQSLVSRLL AQGSELGLEL VFVWNRDPGR MAGSVPPALQ 70 80 90 100 110 120 LEDLTTLEER HPDLVVEVAH PKIIHESGVQ ILRHANLLVG SPSALADQTT ERQLLEASNH 130 140 150 160 170 180 WGHTVFVARG ALWGCEDISR LDAAGGLQSL RVTMATHPDG FRLEGPLAAA HSSGPRTVLY 190 200 210 220 230 240 EGPVRGLCPL APRNSNTMAA AALAAPSLGF DRVIGVLVAD LSLTDMHVVD VELTGPQGPQ 250 260 270 280 290 AAALPCTPTE RTQPSLALSL APLLLQPSGT AYWAAVSFPP DLGSASAEFP PLPLLSP

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC088252 mRNA. Translation: AAH88252.1.
RefSeq	NP_001009643.2.
UniGene	Rn.54118
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000019424. Rattus norvegicus. [Contig view]
GeneID	292875.
KEGG	rno:292875.
Organism-specific databases
RGD	1310111. RGD1310111.
Phylogenomic databases
HOVERGEN	Q5I0J9.
Gene expression databases
ArrayExpress	Q5I0J9.
GermOnline	ENSRNOG00000019424. Rattus norvegicus.
Family and domain databases
InterPro	IPR005106. Asp/hSer_DHase_NAD-bd. IPR002811. Asp_DHase. IPR011182. Asp_DHase_NAD_syn. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProDom	PD017325. Asp_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	634962.

Entry information

Entry name

ASPD_RAT

Accession

Primary (citable) accession number: Q5I0J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	February 15, 2005
Last modified:	November 25, 2008
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)