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Protein

E3 ubiquitin-protein ligase MARCH2

Gene

March2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in endosomal trafficking through interaction with STX6.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri56 – 11661RING-CH-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Endocytosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH2 (EC:6.3.2.-)
Alternative name(s):
Membrane-associated RING finger protein 2
Membrane-associated RING-CH protein II
Short name:
MARCH-II
Gene namesi
Name:March2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1306395. March2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei138 – 15821HelicalSequence analysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246E3 ubiquitin-protein ligase MARCH2PRO_0000274503Add
BLAST

Proteomic databases

PaxDbiQ5I0I2.

Expressioni

Tissue specificityi

Ubiquitously expressed. Present in liver (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ5I0I2. baseline and differential.
GenevisibleiQ5I0I2. RN.

Interactioni

Subunit structurei

Interacts with STX6 and MARCH3.2 Publications

Protein-protein interaction databases

BioGridi263774. 7 interactions.
STRINGi10116.ENSRNOP00000010399.

Structurei

3D structure databases

ProteinModelPortaliQ5I0I2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 RING-CH-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri56 – 11661RING-CH-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1609. Eukaryota.
COG5183. LUCA.
GeneTreeiENSGT00730000110355.
HOGENOMiHOG000293394.
HOVERGENiHBG052411.
InParanoidiQ5I0I2.
KOiK10657.
OMAiPSDCPFC.
OrthoDBiEOG7B05F1.
PhylomeDBiQ5I0I2.
TreeFamiTF319557.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiPF12906. RINGv. 1 hit.
[Graphical view]
SMARTiSM00744. RINGv. 1 hit.
[Graphical view]
PROSITEiPS51292. ZF_RING_CH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5I0I2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTGDCCHLP GSLCDCSSSP AFSKVVEATG LGPPQYVAQV TSRDGRLLST
60 70 80 90 100
VIRALDTPSD CPFCRICHEG ANGENLLSPC GCTGTLGAVH KSCLEKWLSS
110 120 130 140 150
SNTSYCELCH TEFAVEKRPR PLTEWLKDPG PRTEKRTLCC DMVCFVFITP
160 170 180 190 200
LAAISGWLCL RGAQDHLRLH SRLEAVGLIA LTIALFTIYV LWTLVSFRYH
210 220 230 240
CQLYSEWRKT NQKVRLKIRE ADGSEDPHHS LLATGLLKKV AEETPV
Length:246
Mass (Da):27,169
Last modified:February 15, 2005 - v1
Checksum:i346711F2FF28FF25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB048838 mRNA. Translation: BAD89357.1.
BC088286 mRNA. Translation: AAH88286.1.
RefSeqiNP_001029280.1. NM_001034108.1.
UniGeneiRn.145219.

Genome annotation databases

EnsembliENSRNOT00000010399; ENSRNOP00000010399; ENSRNOG00000007769.
GeneIDi362849.
KEGGirno:362849.
UCSCiRGD:1306395. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB048838 mRNA. Translation: BAD89357.1.
BC088286 mRNA. Translation: AAH88286.1.
RefSeqiNP_001029280.1. NM_001034108.1.
UniGeneiRn.145219.

3D structure databases

ProteinModelPortaliQ5I0I2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi263774. 7 interactions.
STRINGi10116.ENSRNOP00000010399.

Proteomic databases

PaxDbiQ5I0I2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010399; ENSRNOP00000010399; ENSRNOG00000007769.
GeneIDi362849.
KEGGirno:362849.
UCSCiRGD:1306395. rat.

Organism-specific databases

CTDi51257.
RGDi1306395. March2.

Phylogenomic databases

eggNOGiKOG1609. Eukaryota.
COG5183. LUCA.
GeneTreeiENSGT00730000110355.
HOGENOMiHOG000293394.
HOVERGENiHBG052411.
InParanoidiQ5I0I2.
KOiK10657.
OMAiPSDCPFC.
OrthoDBiEOG7B05F1.
PhylomeDBiQ5I0I2.
TreeFamiTF319557.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi681518.
PROiQ5I0I2.

Gene expression databases

ExpressionAtlasiQ5I0I2. baseline and differential.
GenevisibleiQ5I0I2. RN.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiPF12906. RINGv. 1 hit.
[Graphical view]
SMARTiSM00744. RINGv. 1 hit.
[Graphical view]
PROSITEiPS51292. ZF_RING_CH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking."
    Nakamura N., Fukuda H., Kato A., Hirose S.
    Mol. Biol. Cell 16:1696-1710(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH STX6, FUNCTION.
    Tissue: Small intestine.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  3. "MARCH-III is a novel component of endosomes with properties similar to those of MARCH-II."
    Fukuda H., Nakamura N., Hirose S.
    J. Biochem. 139:137-145(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MARCH3.

Entry informationi

Entry nameiMARH2_RAT
AccessioniPrimary (citable) accession number: Q5I0I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 15, 2005
Last modified: May 11, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.