Q5I0H3 (SUMO1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Small ubiquitin-related modifier 1
Short name=SUMO-1

Gene names

Name:

Sumo1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development By similarity.
Subunit structure	Interacts with SAE2, UBE2I, RANBP2, PIAS1 and PIAS2. Covalently attached to a number of proteins such as IKFZ1, PML, RANGAP1, HIPK2, SP100, p53, p73-alpha, MDM2, JUN and DNMT3B. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, PIAS1, EXOSC9, TDG, RAD51 and RAD52. Interacts with USP25 (via ts SIM domain); the interaction weakly sumoylates USP25 By similarity. Interacts with PARK2. Ref.2
Subcellular location	Nucleus membrane By similarity. Nucleus speckle By similarity. Cytoplasm By similarity. Note: Recruited by BCL11A into the nuclear body By similarity.
Post-translational modification	Cleavage of precursor form by SENP1 or SENP2 is necessary for function By similarity. Polymeric SUMO1 chains undergo polyubiquitination by RNF4 By similarity.
Sequence similarities	Belongs to the ubiquitin family. SUMO subfamily. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Membrane Nucleus
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	palate development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell dendrite Inferred from direct assay. Source: RGD nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from direct assay. Source: RGD
Molecular function	ubiquitin protein ligase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 97	96	Small ubiquitin-related modifier 1		PRO_0000267610
	Propeptide	98 – 101	4	By similarity		PRO_0000267611
Regions
	Domain	20 – 97	78	Ubiquitin-like
Sites
	Site	36	1	Interaction with PIAS2 By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	2	1	Phosphoserine By similarity
	Modified residue	9	1	Phosphoserine By similarity
	Cross-link	7		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) By similarity
	Cross-link	25		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO-1) By similarity
	Cross-link	97		Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5I0H3 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 89BE97D2D054FB33	FASTA	101	11,557
	10 20 30 40 50 60 MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN 70 80 90 100 SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGGHST V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[2]	"Functional modulation of parkin through physical interaction with SUMO-1." Um J.W., Chung K.C. J. Neurosci. Res. 84:1543-1554(2006) [PubMed: 16955485] [Abstract] Cited for: INTERACTION WITH PARK2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC088322 mRNA. Translation: AAH88322.1.
IPI	IPI00358028.
RefSeq	NP_001009672.1. NM_001009672.1.
UniGene	Rn.1221.
3D structure databases
HSSP	HSSP built from PDB template 1A5R based on UniProtKB P63165.
ProteinModelPortal	Q5I0H3.
SMR	Q5I0H3. Positions 1-101.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46501N.
MINT	MINT-4651872.
STRING	Q5I0H3.
Proteomic databases
PRIDE	Q5I0H3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000022047; ENSRNOP00000022048; ENSRNOG00000016133.
GeneID	301442.
KEGG	rno:301442.
UCSC	NM_001009672. rat.
Organism-specific databases
CTD	7341.
RGD	1306919. Sumo1.
Phylogenomic databases
eggNOG	maNOG20287.
GeneTree	ENSGT00390000018808.
HOVERGEN	HBG053025.
InParanoid	Q5I0H3.
OrthoDB	EOG42JNSX.
PhylomeDB	Q5I0H3.
Gene expression databases
ArrayExpress	Q5I0H3.
Genevestigator	Q5I0H3.
GermOnline	ENSRNOG00000016133. Rattus norvegicus.
Family and domain databases
InterPro	IPR000626. Ubiquitin. IPR019955. Ubiquitin_supergroup. [Graphical view]
KO	K12160.
Pfam	PF00240. ubiquitin. 1 hit. [Graphical view]
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS50053. UBIQUITIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	648746.

Entry information

Entry name

SUMO1_RAT

Accession

Primary (citable) accession number: Q5I0H3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	February 15, 2005
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program