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Protein

BRCA1-A complex subunit Abraxas

Gene

Fam175a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA damage response and double-strand break (DSB) repair. Component of the BRCA1-A complex, acting as a central scaffold protein that assembles the various components of the complex and mediates the recruitment of BRCA1. The BRCA1-A complex specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesion sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at DSBs. This complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
BRCA1-A complex subunit Abraxas
Alternative name(s):
Coiled-coil domain-containing protein 98
Protein FAM175A
Gene namesi
Name:Fam175a
Synonyms:Abra1, Ccdc98
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1305287. Fam175a.

Subcellular locationi

  • Nucleus By similarity

  • Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002785771 – 405BRCA1-A complex subunit AbraxasAdd BLAST405

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48PhosphoserineBy similarity1
Modified residuei382PhosphoserineBy similarity1
Modified residuei383PhosphoserineBy similarity1
Modified residuei392PhosphoserineCombined sources1
Modified residuei402PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation of Ser-402 of the pSXXF motif by ATM or ATR constitutes a specific recognition motif for the BRCT domain of BRCA1.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5I0F1.
PRIDEiQ5I0F1.

PTM databases

iPTMnetiQ5I0F1.
PhosphoSitePlusiQ5I0F1.

Interactioni

Subunit structurei

Component of the ARISC complex, at least composed of UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the complex, interacts directly with UIMC1/RAP80, BRCC3/BRCC36 and BRE/BRCC45. Homodimer. Interacts directly (when phosphorylated at Ser-402) with BRCA1. The phosphorylated homodimer can interact directly with two BRCA1 chains, giving rise to a heterotetramer. Binds polyubiquitin.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002956.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 121MPN-likeAdd BLAST111

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili208 – 262Sequence analysisAdd BLAST55

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi402 – 405pSXXF motifCurated4

Domaini

The MPN-like region is similar to the MPN (JAB/Mov34) domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.By similarity

Sequence similaritiesi

Belongs to the FAM175 family. Abraxas subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG59. Eukaryota.
ENOG4110D5E. LUCA.
HOGENOMiHOG000112448.
HOVERGENiHBG095943.
InParanoidiQ5I0F1.
KOiK20774.
PhylomeDBiQ5I0F1.

Family and domain databases

InterProiIPR023238. FAM175.
IPR023239. FAM175_BRCA1-A_cplx_Abraxas_su.
[Graphical view]
PRINTSiPR02052. ABRAXAS.
PR02051. PROTEINF175.

Sequencei

Sequence statusi: Complete.

Q5I0F1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGESTLGVL SGFVLGALTF QHLNTDSDTE GLLLGEMKGE AKNSITDSQM
60 70 80 90 100
DSVKVVYTID IQKYIPCYRL FSFYNSLGEV NEHALKKILS NVKKTVVGWY
110 120 130 140 150
KFRRHSDQIM TFREQLLHRN LQTHLSSPEL VFLLLTPSIT TESCCTHCLE
160 170 180 190 200
HGLYKPQSGL FHKVPLVVTN LGMSDQLGYK TESVSCTSTV FSRAVRTYSS
210 220 230 240 250
QFFNEDGSLK EVRKINEMYA AIQEELKTIC QKVEQSEREV EKLLMDVNRL
260 270 280 290 300
KEVRKKQQAQ AKGAGEKSQN HPQENILLCQ ALRTFFPESR VLHSCVISLK
310 320 330 340 350
NRHISHSGCN TDHHLDVVDK LTLMVEYVYS PEASPAPAAP LSKRKALDTQ
360 370 380 390 400
DQWPAKRPRL LESESRPGPA FRGSHQDKAS SSSLDIDTEV GSPEDDTDYP

RSPTF
Length:405
Mass (Da):45,690
Last modified:February 15, 2005 - v1
Checksum:iB9A0C4BF979D9473
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088408 mRNA. Translation: AAH88408.1.
RefSeqiNP_001009633.1. NM_001009633.1.
UniGeneiRn.106875.

Genome annotation databases

GeneIDi289468.
KEGGirno:289468.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088408 mRNA. Translation: AAH88408.1.
RefSeqiNP_001009633.1. NM_001009633.1.
UniGeneiRn.106875.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002956.

PTM databases

iPTMnetiQ5I0F1.
PhosphoSitePlusiQ5I0F1.

Proteomic databases

PaxDbiQ5I0F1.
PRIDEiQ5I0F1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi289468.
KEGGirno:289468.

Organism-specific databases

CTDi84142.
RGDi1305287. Fam175a.

Phylogenomic databases

eggNOGiENOG410IG59. Eukaryota.
ENOG4110D5E. LUCA.
HOGENOMiHOG000112448.
HOVERGENiHBG095943.
InParanoidiQ5I0F1.
KOiK20774.
PhylomeDBiQ5I0F1.

Miscellaneous databases

PROiQ5I0F1.

Family and domain databases

InterProiIPR023238. FAM175.
IPR023239. FAM175_BRCA1-A_cplx_Abraxas_su.
[Graphical view]
PRINTSiPR02052. ABRAXAS.
PR02051. PROTEINF175.
ProtoNetiSearch...

Entry informationi

Entry nameiF175A_RAT
AccessioniPrimary (citable) accession number: Q5I0F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 15, 2005
Last modified: November 30, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.