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Protein

Transmembrane emp24 domain-containing protein 9

Gene

Tmed9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer-dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-6807878 COPI-mediated anterograde transport
R-RNO-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 9
Alternative name(s):
p24 family protein alpha-2
Short name:
p24alpha2
Gene namesi
Name:Tmed9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi1307627 Tmed9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini38 – 202LumenalSequence analysisAdd BLAST165
Transmembranei203 – 222HelicalSequence analysisAdd BLAST20
Topological domaini223 – 235CytoplasmicSequence analysisAdd BLAST13

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 371 PublicationAdd BLAST37
ChainiPRO_000041398838 – 235Transmembrane emp24 domain-containing protein 9Add BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi125N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei160N6-acetyllysineBy similarity1

Post-translational modificationi

N-linked glycosylated containing high mannose.By similarity

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

PaxDbiQ5I0E7
PRIDEiQ5I0E7

Expressioni

Gene expression databases

BgeeiENSRNOG00000021882
GenevisibleiQ5I0E7 RN

Interactioni

Subunit structurei

Monomer and homodimer in endoplasmic reticulum. Predominantly monomeric and to lesser extent homodimeric in endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED7 and TMED10. Interacts with TMED5. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED9. Interacts with PTPN2 and SPAST (By similarity). Interacts with STX17; the interaction is direct.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gorasp2Q9R0643EBI-920903,EBI-4422912

GO - Molecular functioni

Protein-protein interaction databases

BioGridi262515, 5 interactors
IntActiQ5I0E7, 4 interactors
STRINGi10116.ENSRNOP00000030668

Structurei

3D structure databases

SMRiQ5I0E7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 145GOLDPROSITE-ProRule annotationAdd BLAST99

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni121 – 160Required for interaction with STX17By similarityAdd BLAST40

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili154 – 184Sequence analysisAdd BLAST31

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi228 – 235COPI vesicle coat-bindingSequence analysis8
Motifi228 – 229COPII vesicle coat-bindingSequence analysis2

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1690 Eukaryota
ENOG410XPUW LUCA
GeneTreeiENSGT00430000030848
HOGENOMiHOG000160228
HOVERGENiHBG105357
InParanoidiQ5I0E7
KOiK20346
OMAiVLWWSIV
OrthoDBiEOG091G0JDW
PhylomeDBiQ5I0E7
TreeFamiTF314123

Family and domain databases

InterProiView protein in InterPro
IPR009038 GOLD_dom
IPR015720 TMP21-related
PANTHERiPTHR22811 PTHR22811, 1 hit
PfamiView protein in Pfam
PF01105 EMP24_GP25L, 1 hit
SMARTiView protein in SMART
SM01190 EMP24_GP25L, 1 hit
PROSITEiView protein in PROSITE
PS50866 GOLD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5I0E7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVRGVRVV GTSPGLLLGR GMRAFLLLLC LAARGGALYF HIGETEKKCF
60 70 80 90 100
IEEIPDETMV IGNYRTQLYD KQREEYQPAT PGLGMFVEVK DPEDKVILAR
110 120 130 140 150
QYGSEGRFTF TSHTPGEHQI CLHSNSTKFS LFAGGMLRVH LDIQVGEHAN
160 170 180 190 200
DYAEIAAKDK LSELQLRVRQ LVEQVEQIQK EQNYQRWREE RFRQTSESTN
210 220 230
QRVLWWSILQ TLILVAIGVW QMRHLKSFFE AKKLV
Length:235
Mass (Da):27,028
Last modified:February 15, 2005 - v1
Checksum:i32F0D1DCC9EFBA6E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38L → I AA sequence (PubMed:9472029).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474032 Genomic DNA Translation: EDL93964.1
BC088422 mRNA Translation: AAH88422.1
RefSeqiNP_001009703.1, NM_001009703.1
UniGeneiRn.9386

Genome annotation databases

EnsembliENSRNOT00000036140; ENSRNOP00000030668; ENSRNOG00000021882
GeneIDi361207
KEGGirno:361207
UCSCiRGD:1307627 rat

Similar proteinsi

Entry informationi

Entry nameiTMED9_RAT
AccessioniPrimary (citable) accession number: Q5I0E7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: February 15, 2005
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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