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Protein

Transmembrane emp24 domain-containing protein 9

Gene

Tmed9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer-dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 9
Alternative name(s):
p24 family protein alpha-2
Short name:
p24alpha2
Gene namesi
Name:Tmed9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi1307627. Tmed9.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini38 – 202LumenalSequence analysisAdd BLAST165
Transmembranei203 – 222HelicalSequence analysisAdd BLAST20
Topological domaini223 – 235CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 371 PublicationAdd BLAST37
ChainiPRO_000041398838 – 235Transmembrane emp24 domain-containing protein 9Add BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi125N-linked (GlcNAc...)Sequence analysis1
Modified residuei160N6-acetyllysineBy similarity1

Post-translational modificationi

N-linked glycosylated containing high mannose.By similarity

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

PaxDbiQ5I0E7.
PRIDEiQ5I0E7.

Expressioni

Gene expression databases

BgeeiENSRNOG00000021882.
GenevisibleiQ5I0E7. RN.

Interactioni

Subunit structurei

Monomer and homodimer in endoplasmic reticulum. Predominantly monomeric and to lesser extent homodimeric in endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED7 and TMED10. Interacts with TMED5. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED9. Interacts with PTPN2 and SPAST (By similarity). Interacts with STX17; the interaction is direct.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gorasp2Q9R0643EBI-920903,EBI-4422912

Protein-protein interaction databases

BioGridi262515. 5 interactors.
IntActiQ5I0E7. 4 interactors.
MINTiMINT-4655706.
STRINGi10116.ENSRNOP00000030668.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 145GOLDPROSITE-ProRule annotationAdd BLAST99

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni121 – 160Required for interaction with STX17By similarityAdd BLAST40

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili154 – 184Sequence analysisAdd BLAST31

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi228 – 235COPI vesicle coat-bindingSequence analysis8
Motifi228 – 229COPII vesicle coat-bindingSequence analysis2

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1690. Eukaryota.
ENOG410XPUW. LUCA.
GeneTreeiENSGT00430000030848.
HOGENOMiHOG000160228.
HOVERGENiHBG105357.
InParanoidiQ5I0E7.
KOiK20346.
OMAiQWICLAL.
OrthoDBiEOG091G0JDW.
PhylomeDBiQ5I0E7.
TreeFamiTF314123.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5I0E7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVRGVRVV GTSPGLLLGR GMRAFLLLLC LAARGGALYF HIGETEKKCF
60 70 80 90 100
IEEIPDETMV IGNYRTQLYD KQREEYQPAT PGLGMFVEVK DPEDKVILAR
110 120 130 140 150
QYGSEGRFTF TSHTPGEHQI CLHSNSTKFS LFAGGMLRVH LDIQVGEHAN
160 170 180 190 200
DYAEIAAKDK LSELQLRVRQ LVEQVEQIQK EQNYQRWREE RFRQTSESTN
210 220 230
QRVLWWSILQ TLILVAIGVW QMRHLKSFFE AKKLV
Length:235
Mass (Da):27,028
Last modified:February 15, 2005 - v1
Checksum:i32F0D1DCC9EFBA6E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38L → I AA sequence (PubMed:9472029).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474032 Genomic DNA. Translation: EDL93964.1.
BC088422 mRNA. Translation: AAH88422.1.
RefSeqiNP_001009703.1. NM_001009703.1.
UniGeneiRn.9386.

Genome annotation databases

EnsembliENSRNOT00000036140; ENSRNOP00000030668; ENSRNOG00000021882.
GeneIDi361207.
KEGGirno:361207.
UCSCiRGD:1307627. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474032 Genomic DNA. Translation: EDL93964.1.
BC088422 mRNA. Translation: AAH88422.1.
RefSeqiNP_001009703.1. NM_001009703.1.
UniGeneiRn.9386.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi262515. 5 interactors.
IntActiQ5I0E7. 4 interactors.
MINTiMINT-4655706.
STRINGi10116.ENSRNOP00000030668.

Proteomic databases

PaxDbiQ5I0E7.
PRIDEiQ5I0E7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000036140; ENSRNOP00000030668; ENSRNOG00000021882.
GeneIDi361207.
KEGGirno:361207.
UCSCiRGD:1307627. rat.

Organism-specific databases

CTDi54732.
RGDi1307627. Tmed9.

Phylogenomic databases

eggNOGiKOG1690. Eukaryota.
ENOG410XPUW. LUCA.
GeneTreeiENSGT00430000030848.
HOGENOMiHOG000160228.
HOVERGENiHBG105357.
InParanoidiQ5I0E7.
KOiK20346.
OMAiQWICLAL.
OrthoDBiEOG091G0JDW.
PhylomeDBiQ5I0E7.
TreeFamiTF314123.

Enzyme and pathway databases

ReactomeiR-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiQ5I0E7.

Gene expression databases

BgeeiENSRNOG00000021882.
GenevisibleiQ5I0E7. RN.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTMED9_RAT
AccessioniPrimary (citable) accession number: Q5I0E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.