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Protein

RNA polymerase II subunit B1 CTD phosphatase Rpap2

Gene

Rpap2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase that displays CTD phosphatase activity and regulates transcription of snRNA genes. Recognizes and binds phosphorylated 'Ser-7' of the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and mediates dephosphorylation of 'Ser-5' of the CTD, thereby promoting transcription of snRNA genes (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi100ZincPROSITE-ProRule annotation1
Metal bindingi105ZincPROSITE-ProRule annotation1
Metal bindingi136ZincPROSITE-ProRule annotation1
Metal bindingi140ZincPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri77 – 160RTR1-typePROSITE-ProRule annotationAdd BLAST84

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-6807505. RNA polymerase II transcribes snRNA genes.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II subunit B1 CTD phosphatase Rpap2 (EC:3.1.3.16)
Alternative name(s):
Putative RNA polymerase II-associated protein 2
Gene namesi
Name:Rpap2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi1309034. Rpap2.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Shuttles between the cytoplasm and the nucleus in a CRM1-dependent manner.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002506512 – 609RNA polymerase II subunit B1 CTD phosphatase Rpap2Add BLAST608

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei9PhosphoserineBy similarity1
Modified residuei222PhosphoserineCombined sources1
Modified residuei476PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5I0E6.
PRIDEiQ5I0E6.

PTM databases

iPTMnetiQ5I0E6.
PhosphoSitePlusiQ5I0E6.

Expressioni

Gene expression databases

BgeeiENSRNOG00000023484.
GenevisibleiQ5I0E6. RN.

Interactioni

Subunit structurei

Associates with the RNA polymerase II complex. Interacts with transcribing RNA polymerase II phosphorylated on 'Ser-7' on CTD (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002815.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili33 – 69Sequence analysisAdd BLAST37

Sequence similaritiesi

Belongs to the RPAP2 family.Curated
Contains 1 RTR1-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri77 – 160RTR1-typePROSITE-ProRule annotationAdd BLAST84

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG4780. Eukaryota.
ENOG410ZZZC. LUCA.
GeneTreeiENSGT00390000017965.
HOGENOMiHOG000253960.
HOVERGENiHBG080953.
InParanoidiQ5I0E6.
KOiK20827.
OMAiCYRASKY.
OrthoDBiEOG091G0TLM.
PhylomeDBiQ5I0E6.
TreeFamiTF331431.

Family and domain databases

InterProiIPR007308. DUF408.
[Graphical view]
PfamiPF04181. RPAP2_Rtr1. 1 hit.
[Graphical view]
PROSITEiPS51479. ZF_RTR1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5I0E6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADSAVPYSL GPSARASSTH RVATGTKQTS ALKRRDASKR QAELEAALQR
60 70 80 90 100
KVESERRAVR LVEQLLEENI TEEFLKECGM FITPAHYSDV VDERAIIKLC
110 120 130 140 150
GYPLCQKKLG VIPKQKYRIS TKTNKVYDIT ERKSFCSNFC YKASKFFEAQ
160 170 180 190 200
IPKTPVWVRE EERPPEFQLL KKGQSGCSGE VVQFFRDAVT AADVDAYGAF
210 220 230 240 250
DAQCEPASSS TWSERASDER ASDEEGPGFV SSLLPGNRPK AVGTKPQPHR
260 270 280 290 300
QSSTVKKKAA QKMTSKHGEQ TVSEVTEQLS NCRLDSQEKV ATCKLPAKKE
310 320 330 340 350
NTQISSPGPL CDRLNTSTVS ENKHSVSQVT LVGISKKSAE HFRSKFAKSN
360 370 380 390 400
PGSGSASGLV QVRPEVAKAN LLRVLKDTLT EWKTDETLKF LYGQDHGSVC
410 420 430 440 450
LQPSAASGPD EELDEDDISC QAQNTLDETL PFRGSDTAIK PLPSYESLKK
460 470 480 490 500
ETEMLNLRVR EFYRGRCVLN EDSTKSQDSK ENELQRDPSF PLIDSSSQNQ
510 520 530 540 550
IRRRIVLEKL SKVLPGLLGP LQITMGDIYT ELKNLVQTFR LSNRNIIHKP
560 570 580 590 600
VEWTLIAVVL LSLLTPILGI QKHSPKNVVF TQFIATLLTE LHLKCEDLEN

LAMIFRTSC
Length:609
Mass (Da):67,892
Last modified:February 15, 2005 - v1
Checksum:iAB4E505B6037636D
GO
Isoform 2 (identifier: Q5I0E6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     214-218: Missing.

Show »
Length:604
Mass (Da):67,334
Checksum:i6DC2B969BA54B3CD
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042584214 – 218Missing in isoform 2. Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088426 mRNA. Translation: AAH88426.1.
RefSeqiNP_001014009.1. NM_001013987.1. [Q5I0E6-1]
UniGeneiRn.49042.

Genome annotation databases

EnsembliENSRNOT00000002815; ENSRNOP00000002815; ENSRNOG00000023484. [Q5I0E6-1]
GeneIDi305120.
KEGGirno:305120.
UCSCiRGD:1309034. rat. [Q5I0E6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088426 mRNA. Translation: AAH88426.1.
RefSeqiNP_001014009.1. NM_001013987.1. [Q5I0E6-1]
UniGeneiRn.49042.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002815.

PTM databases

iPTMnetiQ5I0E6.
PhosphoSitePlusiQ5I0E6.

Proteomic databases

PaxDbiQ5I0E6.
PRIDEiQ5I0E6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002815; ENSRNOP00000002815; ENSRNOG00000023484. [Q5I0E6-1]
GeneIDi305120.
KEGGirno:305120.
UCSCiRGD:1309034. rat. [Q5I0E6-1]

Organism-specific databases

CTDi79871.
RGDi1309034. Rpap2.

Phylogenomic databases

eggNOGiKOG4780. Eukaryota.
ENOG410ZZZC. LUCA.
GeneTreeiENSGT00390000017965.
HOGENOMiHOG000253960.
HOVERGENiHBG080953.
InParanoidiQ5I0E6.
KOiK20827.
OMAiCYRASKY.
OrthoDBiEOG091G0TLM.
PhylomeDBiQ5I0E6.
TreeFamiTF331431.

Enzyme and pathway databases

ReactomeiR-RNO-6807505. RNA polymerase II transcribes snRNA genes.

Miscellaneous databases

PROiQ5I0E6.

Gene expression databases

BgeeiENSRNOG00000023484.
GenevisibleiQ5I0E6. RN.

Family and domain databases

InterProiIPR007308. DUF408.
[Graphical view]
PfamiPF04181. RPAP2_Rtr1. 1 hit.
[Graphical view]
PROSITEiPS51479. ZF_RTR1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPAP2_RAT
AccessioniPrimary (citable) accession number: Q5I0E6
Secondary accession number(s): D4A5J9, D4A672
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: February 15, 2005
Last modified: November 30, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.