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Q5I0E6

- RPAP2_RAT

UniProt

Q5I0E6 - RPAP2_RAT

Protein

RNA polymerase II subunit B1 CTD phosphatase Rpap2

Gene

Rpap2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Protein phosphatase that displays CTD phosphatase activity and regulates transcription of snRNA genes. Recognizes and binds phosphorylated 'Ser-7' of the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and mediates dephosphorylation of 'Ser-5' of the CTD, thereby promoting transcription of snRNA genes By similarity.By similarity

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri77 – 16084RTR1-typeAdd
    BLAST

    GO - Molecular functioni

    1. CTD phosphatase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dephosphorylation of RNA polymerase II C-terminal domain Source: UniProtKB
    2. snRNA transcription Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA polymerase II subunit B1 CTD phosphatase Rpap2 (EC:3.1.3.16)
    Alternative name(s):
    Putative RNA polymerase II-associated protein 2
    Gene namesi
    Name:Rpap2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 14

    Organism-specific databases

    RGDi1309034. Rpap2.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Shuttles between the cytoplasm and the nucleus in a CRM1-dependent manner.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. DNA-directed RNA polymerase II, holoenzyme Source: UniProtKB
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 609608RNA polymerase II subunit B1 CTD phosphatase Rpap2PRO_0000250651Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei476 – 4761PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ5I0E6.
    PRIDEiQ5I0E6.

    Expressioni

    Gene expression databases

    GenevestigatoriQ5I0E6.

    Interactioni

    Subunit structurei

    Associates with the RNA polymerase II complex. Interacts with transcribing RNA polymerase II phosphorylated on 'Ser-7' on CTD By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000002815.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili33 – 6937Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RPAP2 family.Curated
    Contains 1 RTR1-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri77 – 16084RTR1-typeAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG241465.
    GeneTreeiENSGT00390000017965.
    HOGENOMiHOG000253960.
    HOVERGENiHBG080953.
    InParanoidiQ5I0E6.
    OMAiIIFRTSC.
    OrthoDBiEOG7XWPQD.
    PhylomeDBiQ5I0E6.
    TreeFamiTF331431.

    Family and domain databases

    InterProiIPR007308. DUF408.
    [Graphical view]
    PfamiPF04181. RPAP2_Rtr1. 1 hit.
    [Graphical view]
    PROSITEiPS51479. ZF_RTR1. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5I0E6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MADSAVPYSL GPSARASSTH RVATGTKQTS ALKRRDASKR QAELEAALQR    50
    KVESERRAVR LVEQLLEENI TEEFLKECGM FITPAHYSDV VDERAIIKLC 100
    GYPLCQKKLG VIPKQKYRIS TKTNKVYDIT ERKSFCSNFC YKASKFFEAQ 150
    IPKTPVWVRE EERPPEFQLL KKGQSGCSGE VVQFFRDAVT AADVDAYGAF 200
    DAQCEPASSS TWSERASDER ASDEEGPGFV SSLLPGNRPK AVGTKPQPHR 250
    QSSTVKKKAA QKMTSKHGEQ TVSEVTEQLS NCRLDSQEKV ATCKLPAKKE 300
    NTQISSPGPL CDRLNTSTVS ENKHSVSQVT LVGISKKSAE HFRSKFAKSN 350
    PGSGSASGLV QVRPEVAKAN LLRVLKDTLT EWKTDETLKF LYGQDHGSVC 400
    LQPSAASGPD EELDEDDISC QAQNTLDETL PFRGSDTAIK PLPSYESLKK 450
    ETEMLNLRVR EFYRGRCVLN EDSTKSQDSK ENELQRDPSF PLIDSSSQNQ 500
    IRRRIVLEKL SKVLPGLLGP LQITMGDIYT ELKNLVQTFR LSNRNIIHKP 550
    VEWTLIAVVL LSLLTPILGI QKHSPKNVVF TQFIATLLTE LHLKCEDLEN 600
    LAMIFRTSC 609
    Length:609
    Mass (Da):67,892
    Last modified:February 15, 2005 - v1
    Checksum:iAB4E505B6037636D
    GO
    Isoform 2 (identifier: Q5I0E6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         214-218: Missing.

    Show »
    Length:604
    Mass (Da):67,334
    Checksum:i6DC2B969BA54B3CD
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei214 – 2185Missing in isoform 2. CuratedVSP_042584

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC088426 mRNA. Translation: AAH88426.1.
    RefSeqiNP_001014009.1. NM_001013987.1. [Q5I0E6-1]
    XP_006250606.1. XM_006250544.1. [Q5I0E6-1]
    UniGeneiRn.49042.

    Genome annotation databases

    EnsembliENSRNOT00000002815; ENSRNOP00000002815; ENSRNOG00000023484. [Q5I0E6-1]
    GeneIDi305120.
    KEGGirno:305120.
    UCSCiRGD:1309034. rat. [Q5I0E6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC088426 mRNA. Translation: AAH88426.1 .
    RefSeqi NP_001014009.1. NM_001013987.1. [Q5I0E6-1 ]
    XP_006250606.1. XM_006250544.1. [Q5I0E6-1 ]
    UniGenei Rn.49042.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000002815.

    Proteomic databases

    PaxDbi Q5I0E6.
    PRIDEi Q5I0E6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000002815 ; ENSRNOP00000002815 ; ENSRNOG00000023484 . [Q5I0E6-1 ]
    GeneIDi 305120.
    KEGGi rno:305120.
    UCSCi RGD:1309034. rat. [Q5I0E6-1 ]

    Organism-specific databases

    CTDi 79871.
    RGDi 1309034. Rpap2.

    Phylogenomic databases

    eggNOGi NOG241465.
    GeneTreei ENSGT00390000017965.
    HOGENOMi HOG000253960.
    HOVERGENi HBG080953.
    InParanoidi Q5I0E6.
    OMAi IIFRTSC.
    OrthoDBi EOG7XWPQD.
    PhylomeDBi Q5I0E6.
    TreeFami TF331431.

    Miscellaneous databases

    NextBioi 654102.
    PROi Q5I0E6.

    Gene expression databases

    Genevestigatori Q5I0E6.

    Family and domain databases

    InterProi IPR007308. DUF408.
    [Graphical view ]
    Pfami PF04181. RPAP2_Rtr1. 1 hit.
    [Graphical view ]
    PROSITEi PS51479. ZF_RTR1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
      Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
      , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
      Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung.

    Entry informationi

    Entry nameiRPAP2_RAT
    AccessioniPrimary (citable) accession number: Q5I0E6
    Secondary accession number(s): D4A5J9, D4A672
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3