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Q5I0E6 (RPAP2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA polymerase II subunit B1 CTD phosphatase Rpap2

EC=3.1.3.16
Alternative name(s):
Putative RNA polymerase II-associated protein 2
Gene names
Name:Rpap2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length609 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protein phosphatase that displays CTD phosphatase activity and regulates transcription of snRNA genes. Recognizes and binds phosphorylated 'Ser-7' of the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and mediates dephosphorylation of 'Ser-5' of the CTD, thereby promoting transcription of snRNA genes By similarity.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Subunit structure

Associates with the RNA polymerase II complex. Interacts with transcribing RNA polymerase II phosphorylated on 'Ser-7' on CTD By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Shuttles between the cytoplasm and the nucleus in a CRM1-dependent manner By similarity.

Sequence similarities

Belongs to the RPAP2 family.

Contains 1 RTR1-type zinc finger.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5I0E6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5I0E6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     214-218: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 609608RNA polymerase II subunit B1 CTD phosphatase Rpap2
PRO_0000250651

Regions

Zinc finger77 – 16084RTR1-type
Coiled coil33 – 6937 Potential

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue4761Phosphoserine By similarity

Natural variations

Alternative sequence214 – 2185Missing in isoform 2.
VSP_042584

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: AB4E505B6037636D

FASTA60967,892
        10         20         30         40         50         60 
MADSAVPYSL GPSARASSTH RVATGTKQTS ALKRRDASKR QAELEAALQR KVESERRAVR 

        70         80         90        100        110        120 
LVEQLLEENI TEEFLKECGM FITPAHYSDV VDERAIIKLC GYPLCQKKLG VIPKQKYRIS 

       130        140        150        160        170        180 
TKTNKVYDIT ERKSFCSNFC YKASKFFEAQ IPKTPVWVRE EERPPEFQLL KKGQSGCSGE 

       190        200        210        220        230        240 
VVQFFRDAVT AADVDAYGAF DAQCEPASSS TWSERASDER ASDEEGPGFV SSLLPGNRPK 

       250        260        270        280        290        300 
AVGTKPQPHR QSSTVKKKAA QKMTSKHGEQ TVSEVTEQLS NCRLDSQEKV ATCKLPAKKE 

       310        320        330        340        350        360 
NTQISSPGPL CDRLNTSTVS ENKHSVSQVT LVGISKKSAE HFRSKFAKSN PGSGSASGLV 

       370        380        390        400        410        420 
QVRPEVAKAN LLRVLKDTLT EWKTDETLKF LYGQDHGSVC LQPSAASGPD EELDEDDISC 

       430        440        450        460        470        480 
QAQNTLDETL PFRGSDTAIK PLPSYESLKK ETEMLNLRVR EFYRGRCVLN EDSTKSQDSK 

       490        500        510        520        530        540 
ENELQRDPSF PLIDSSSQNQ IRRRIVLEKL SKVLPGLLGP LQITMGDIYT ELKNLVQTFR 

       550        560        570        580        590        600 
LSNRNIIHKP VEWTLIAVVL LSLLTPILGI QKHSPKNVVF TQFIATLLTE LHLKCEDLEN 


LAMIFRTSC 

« Hide

Isoform 2 [UniParc].

Checksum: 6DC2B969BA54B3CD
Show »

FASTA60467,334

References

[1]"Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. expand/collapse author list , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC088426 mRNA. Translation: AAH88426.1.
RefSeqNP_001014009.1. NM_001013987.1. [Q5I0E6-1]
XP_006250606.1. XM_006250544.1. [Q5I0E6-1]
UniGeneRn.49042.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000002815.

Proteomic databases

PaxDbQ5I0E6.
PRIDEQ5I0E6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000002815; ENSRNOP00000002815; ENSRNOG00000023484. [Q5I0E6-1]
GeneID305120.
KEGGrno:305120.
UCSCRGD:1309034. rat. [Q5I0E6-1]

Organism-specific databases

CTD79871.
RGD1309034. Rpap2.

Phylogenomic databases

eggNOGNOG241465.
GeneTreeENSGT00390000017965.
HOGENOMHOG000253960.
HOVERGENHBG080953.
InParanoidQ5I0E6.
OMAIIFRTSC.
OrthoDBEOG7XWPQD.
PhylomeDBQ5I0E6.
TreeFamTF331431.

Gene expression databases

GenevestigatorQ5I0E6.

Family and domain databases

InterProIPR007308. DUF408.
[Graphical view]
PfamPF04181. RPAP2_Rtr1. 1 hit.
[Graphical view]
PROSITEPS51479. ZF_RTR1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio654102.
PROQ5I0E6.

Entry information

Entry nameRPAP2_RAT
AccessionPrimary (citable) accession number: Q5I0E6
Secondary accession number(s): D4A5J9, D4A672
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: February 15, 2005
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families