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Protein

Xaa-Pro dipeptidase

Gene

Pepd

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because of the high level of iminoacids in collagen (By similarity).By similarity

Catalytic activityi

Hydrolysis of Xaa-|-Pro dipeptides; also acts on aminoacyl-hydroxyproline analogs. No action on Pro-|-Pro.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi276 – 2761Manganese 1By similarity
Metal bindingi287 – 2871Manganese 1By similarity
Metal bindingi287 – 2871Manganese 2By similarity
Metal bindingi370 – 3701Manganese 2By similarity
Metal bindingi412 – 4121Manganese 2By similarity
Metal bindingi452 – 4521Manganese 1By similarity
Metal bindingi452 – 4521Manganese 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dipeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Collagen degradation

Keywords - Ligandi

Manganese, Metal-binding

Protein family/group databases

MEROPSiM24.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Xaa-Pro dipeptidase (EC:3.4.13.9)
Short name:
X-Pro dipeptidase
Alternative name(s):
Imidodipeptidase
Peptidase D
Proline dipeptidase
Short name:
Prolidase
Gene namesi
Name:Pepd
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1594571. Pepd.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 492491Xaa-Pro dipeptidasePRO_0000328065Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineSequence analysisBy similarity
Modified residuei167 – 1671PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5I0D7.
PRIDEiQ5I0D7.

PTM databases

iPTMnetiQ5I0D7.
PhosphoSiteiQ5I0D7.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028701.

Structurei

3D structure databases

ProteinModelPortaliQ5I0D7.
SMRiQ5I0D7. Positions 7-482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2737. Eukaryota.
COG0006. LUCA.
HOGENOMiHOG000008763.
HOVERGENiHBG053562.
InParanoidiQ5I0D7.
KOiK14213.
PhylomeDBiQ5I0D7.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
3.90.230.10. 1 hit.
InterProiIPR007865. Aminopep_P_N.
IPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000994. Pept_M24_structural-domain.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF05195. AMP_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
[Graphical view]
SMARTiSM01011. AMP_N. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5I0D7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTVRPSFS LGNETLKVPL ALFALNRQRL CERLRKNGAV QAGSAVVLQG
60 70 80 90 100
GEEMQRYCTD TSIIFRQESF FHWAFGVIES GCYGVIDVDT GKSTLFVPRL
110 120 130 140 150
PASYATWMGK IHSKEHFKEK YAVDDVQYAD EIASVLTSRN PSVLLTLRGV
160 170 180 190 200
NTDSGNVCRE ASFEGISKFT VNNTILHPEI VECRVFKTDM ELEVLRYTNR
210 220 230 240 250
ISSEAHREVM KAVKVGMKEY EMESLFQHYC YSKGGMRHTS YTCICCSGEN
260 270 280 290 300
AAVLHYGHAG APNDRTIKDG DICLFDMGGE YYCFASDITC SFPANGKFTD
310 320 330 340 350
DQKAIYEAVL RSCRTVMSTM KPGVWWPDMH RLADRIHLEE LTRIGLLSGS
360 370 380 390 400
VDAMLQVHLG AVFMPHGLGH FLGLDVHDVG GYPEGVERID EPGLRSLRTA
410 420 430 440 450
RHLEPGMVLT VEPGIYFIDH LLDQALADPA QACFFNQEVL QRFRNFGGVR
460 470 480 490
IEEDVVVTDS GMELLTCVPR TVEEIEACMA GCDKALAPSG PK
Length:492
Mass (Da):54,751
Last modified:February 15, 2005 - v1
Checksum:i97359DB0E3CADB15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088440 mRNA. Translation: AAH88440.1.
RefSeqiNP_001009641.1. NM_001009641.1.
UniGeneiRn.101639.

Genome annotation databases

GeneIDi292808.
KEGGirno:292808.
UCSCiRGD:1594571. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088440 mRNA. Translation: AAH88440.1.
RefSeqiNP_001009641.1. NM_001009641.1.
UniGeneiRn.101639.

3D structure databases

ProteinModelPortaliQ5I0D7.
SMRiQ5I0D7. Positions 7-482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028701.

Protein family/group databases

MEROPSiM24.007.

PTM databases

iPTMnetiQ5I0D7.
PhosphoSiteiQ5I0D7.

Proteomic databases

PaxDbiQ5I0D7.
PRIDEiQ5I0D7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi292808.
KEGGirno:292808.
UCSCiRGD:1594571. rat.

Organism-specific databases

CTDi5184.
RGDi1594571. Pepd.

Phylogenomic databases

eggNOGiKOG2737. Eukaryota.
COG0006. LUCA.
HOGENOMiHOG000008763.
HOVERGENiHBG053562.
InParanoidiQ5I0D7.
KOiK14213.
PhylomeDBiQ5I0D7.

Miscellaneous databases

PROiQ5I0D7.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
3.90.230.10. 1 hit.
InterProiIPR007865. Aminopep_P_N.
IPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000994. Pept_M24_structural-domain.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF05195. AMP_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
[Graphical view]
SMARTiSM01011. AMP_N. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEPD_RAT
AccessioniPrimary (citable) accession number: Q5I0D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: February 15, 2005
Last modified: June 8, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.