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Protein

Nostrin

Gene

Nostrin

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Multivalent adapter protein which may decrease NOS3 activity by inducing its translocation away from the plasma membrane.By similarity

GO - Molecular functioni

  1. DNA binding Source: Ensembl

GO - Biological processi

  1. endocytosis Source: UniProtKB-KW
  2. negative regulation of transcription, DNA-templated Source: Ensembl
  3. regulation of nitric-oxide synthase activity Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Enzyme and pathway databases

ReactomeiREACT_344407. NOSTRIN mediated eNOS trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Nostrin
Alternative name(s):
BM247
Nitric oxide synthase trafficker
eNOS-trafficking inducer
Gene namesi
Name:Nostrin
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi727920. Nostrin.

Subcellular locationi

Cell membrane By similarity. Cytoplasmic vesicle By similarity. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  2. nucleus Source: Ensembl
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502NostrinPRO_0000289091Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei479 – 4791PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5I0D6.
PRIDEiQ5I0D6.

Expressioni

Tissue specificityi

Over-expressed in brain microcapillaries from spontaneously hypertensive rats.1 Publication

Gene expression databases

GenevestigatoriQ5I0D6.

Interactioni

Subunit structurei

Homotrimer. Interacts with DAB2. Interacts with NOS3, DNM2, WASL and CAV1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062066.

Structurei

3D structure databases

ProteinModelPortaliQ5I0D6.
SMRiQ5I0D6. Positions 432-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 260260F-BARPROSITE-ProRule annotationAdd
BLAST
Domaini438 – 49760SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili160 – 23071Sequence AnalysisAdd
BLAST
Coiled coili305 – 33531Sequence AnalysisAdd
BLAST

Domaini

The SH3 domain mediates interaction with NOS3, DNM2 and WASL.By similarity
The F-BAR domain is necessary for membrane targeting.By similarity

Sequence similaritiesi

Contains 1 F-BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG266189.
GeneTreeiENSGT00510000048120.
HOGENOMiHOG000113830.
HOVERGENiHBG101730.
InParanoidiQ5I0D6.
OMAiFEEDPNN.
OrthoDBiEOG7BCNBJ.
PhylomeDBiQ5I0D6.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR028535. Nostrin.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
[Graphical view]
PANTHERiPTHR14167:SF22. PTHR14167:SF22. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5I0D6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDPLTDCSY NKVYKSLKEF AQNGDNFCKQ ITSVLQQRAN LEISYAKGLQ
60 70 80 90 100
KLAVKLSKAL QSTKKNCLST AWAWASESMK SAADLHQKLG KAIELEAIKP
110 120 130 140 150
THQVLSMQEK KRKSLDNEVE KSANLVINNW NQQIKAKKKL MMSTKKHEAL
160 170 180 190 200
FHLIESSKQS MTQKEKQKLL NKLKKSTEKL EKEDESYYQK NMAGYSTRLK
210 220 230 240 250
WESTLEKCYK SMLELEKERI QLLCNNLNQY SQHISLFGQT LTTCHTQIHC
260 270 280 290 300
AISKVDVEKD IQALMEETAV LSLENKSELL LADYFEEDPK NPMDKERRKS
310 320 330 340 350
LIKPKLWRLQ KDIEKASRDK EGLEQKLKAL ASSASFSDAK SQKDAETLMD
360 370 380 390 400
ENSLKLDLLQ ANSYKLSTVL ADLEQRPKPC HPCSNCIFKW KEKEHSHSYV
410 420 430 440 450
KISRPLLTKR LEKAESKAPA GEQNNPSSSR PGSSVSQGNN QLCKALYTFQ
460 470 480 490 500
ARQDDELNLE KGDIVTIHEK KEEGWWFGSL NGKKGHFPAA YVEELPPKAG

QA
Length:502
Mass (Da):57,352
Last modified:February 15, 2005 - v1
Checksum:iDE8944D1939CCC4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088446 mRNA. Translation: AAH88446.1.
AY032855 mRNA. Translation: AAK64518.1.
RefSeqiNP_001019431.1. NM_001024260.1.
UniGeneiRn.39383.

Genome annotation databases

EnsembliENSRNOT00000067161; ENSRNOP00000062066; ENSRNOG00000006611.
GeneIDi311111.
KEGGirno:311111.
UCSCiRGD:727920. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088446 mRNA. Translation: AAH88446.1.
AY032855 mRNA. Translation: AAK64518.1.
RefSeqiNP_001019431.1. NM_001024260.1.
UniGeneiRn.39383.

3D structure databases

ProteinModelPortaliQ5I0D6.
SMRiQ5I0D6. Positions 432-500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062066.

Proteomic databases

PaxDbiQ5I0D6.
PRIDEiQ5I0D6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000067161; ENSRNOP00000062066; ENSRNOG00000006611.
GeneIDi311111.
KEGGirno:311111.
UCSCiRGD:727920. rat.

Organism-specific databases

CTDi115677.
RGDi727920. Nostrin.

Phylogenomic databases

eggNOGiNOG266189.
GeneTreeiENSGT00510000048120.
HOGENOMiHOG000113830.
HOVERGENiHBG101730.
InParanoidiQ5I0D6.
OMAiFEEDPNN.
OrthoDBiEOG7BCNBJ.
PhylomeDBiQ5I0D6.

Enzyme and pathway databases

ReactomeiREACT_344407. NOSTRIN mediated eNOS trafficking.

Miscellaneous databases

NextBioi662980.
PROiQ5I0D6.

Gene expression databases

GenevestigatoriQ5I0D6.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR028535. Nostrin.
IPR001452. SH3_domain.
IPR013315. Spectrin_alpha_SH3.
[Graphical view]
PANTHERiPTHR14167:SF22. PTHR14167:SF22. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
PR01887. SPECTRNALPHA.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  2. "Altered gene expression in cerebral capillaries of stroke-prone spontaneously hypertensive rats."
    Kirsch T., Wellner M., Luft F.C., Haller H., Lippoldt A.
    Brain Res. 910:106-115(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-143, TISSUE SPECIFICITY.
    Strain: SHRSP.

Entry informationi

Entry nameiNOSTN_RAT
AccessioniPrimary (citable) accession number: Q5I0D6
Secondary accession number(s): Q923J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 15, 2005
Last modified: April 1, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.