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Reviewed, UniProtKB/Swiss-Prot Q5I0C3 (MCCA_RAT)

Last modified January 19, 2010. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
      Short name=MCCase subunit alpha
    EC=6.4.1.4
Alternative name(s):
    3-methylcrotonyl-CoA carboxylase 1
    3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha
    3-methylcrotonyl-CoA carboxylase biotin-containing subunit
Gene names
Name: Mccc1
Synonyms: Mcca
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length715 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA. UniProtKB Q96RQ3

Cofactor

Biotin By similarity. UniProtKB Q96RQ3

Pathway

Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.

Subunit structure

Probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits By similarity. UniProtKB Q96RQ3

Subcellular location

Mitochondrion matrix By similarity UniProtKB Q96RQ3.

Sequence similarities

Contains 1 ATP-grasp domain.

Contains 1 biotin carboxylation domain.

Contains 1 biotinyl-binding domain.

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandATP-binding
Biotin
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

biotin binding

Inferred from electronic annotation. Source: InterPro

methylcrotonoyl-CoA carboxylase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4444Mitochondrion By similarity UniProtKB Q99MR8
Chain45 – 715671Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial UniProtKB Q99MR8
PRO_0000365095

Regions

Domain45 – 490446Biotin carboxylation
Domain163 – 360198ATP-grasp
Domain625 – 71086Biotinyl-binding

Sites

Active site3351 By similarity UniProtKB P24182
Binding site1591ATP By similarity UniProtKB P24182
Binding site2431ATP By similarity UniProtKB P24182
Binding site2781ATP By similarity UniProtKB P24182

Amino acid modifications

Modified residue6771N6-biotinyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5I0C3-1 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 20FB2EAB8664AD8F

FASTA71579,330
        10         20         30         40         50         60 
MAAAALLAAV DRNQLRRVPI LLLQPREWPW KHRTVKYGTT PGGSITKVLI ANRGEIACRV 

        70         80         90        100        110        120 
IRTARKMGVQ SVAVYSEADR NSMHVDMADE AYSIGPAPSQ QSYLAMEKII QVAKSSAAQA 

       130        140        150        160        170        180 
IHPGYGFLSE NMEFAEFCKQ EGIIFIGPPS TAIRDMGIKS TSKSIMAAAG VPVVEGYHGN 

       190        200        210        220        230        240 
DQSDECLKEH AGKIGYPVMI KAIRGGGGKG MRIIRSEKEF QEQLESARRE AKKSFNDDAM 

       250        260        270        280        290        300 
LIEKFVDTPR HVEVQVFGDH HGNAVYLFER DCSVQRRHQK IIEEAPAPGI DPEVRRRLGE 

       310        320        330        340        350        360 
AAVRAAKAVN YVGAGTVEFI MDSKHNFYFM EMNTRLQVEH PVTEMITGTD LVEWQLRIAA 

       370        380        390        400        410        420 
GEKIPLSQEE IPLQGHAFEA RIYAEDPDNN FMPGAGPLVH LSTPPPDMST RIETGVRQGD 

       430        440        450        460        470        480 
EVSVHYDPMI AKLVVWASDR QSALSKLRYS LHQYNIVGLR TNVDFLLRLS GHSEFEAGNV 

       490        500        510        520        530        540 
HTDFIPQHHK DLLPTHSTIA KESVCQAALG LILKEKEMTS AFKLHTQDQF SPFSFSSGRR 

       550        560        570        580        590        600 
LNISYTRNMT LRSGKNDIII AVTYNRDGSY DMQIENKLFR VLGDLSNEDG YTYLKSSVNG 

       610        620        630        640        650        660 
VASKSKFILL DNTIYLFSME GSIEVGIPVP KYLSPVSAEG TQGGTIAPMT GTIEKVFVKA 

       670        680        690        700        710 
GDRVKAGDAL MVMIAMKMEH TIKAPKDGRI KKVFFSEGAQ ANRHAPLVEF EEEEV 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Brown Norway.
Tissue: Kidney.
[2]Maurya D.K., Bhargava P.
Submitted (JAN-2009) to UniProtKB
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC088473 mRNA. Translation: AAH88473.1.
IPIIPI00372191.
RefSeqNP_001009653.1.
UniGeneRn.15587

3D structure databases

HSSPHSSP built from PDB template 1BNC based on UniProtKB P24182.
SMRQ5I0C3. Positions 45-493, 641-714.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5I0C3.

Genome annotation databases

EnsemblENSRNOT00000018942; ENSRNOP00000018942; ENSRNOG00000013293; Rattus norvegicus. [Genome view]
GeneID294972.
KEGGrno:294972.
UCSCNM_001009653. rat.

Organism-specific databases

CTD294972.
RGD1310615. Mccc1.

Phylogenomic databases

eggNOGroNOG07266.
HOVERGENQ5I0C3.
InParanoidQ5I0C3.

Gene expression databases

ArrayExpressQ5I0C3.
GenevestigatorQ5I0C3.

Family and domain databases

InterProIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005479. CarbamoylP_synth_lsu_ATP-bd.
IPR005481. CarbamoylP_synth_lsu_N.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
Gene3DG3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PfamPF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
PROSITEPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. False negative.
PS00867. CPSASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio638797.

Entry information

Entry nameMCCA_RAT
AccessionPrimary (citable) accession number: Q5I0C3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: February 15, 2005
Last modified: January 19, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents