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Q5I0C3 (MCCA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
Short name=MCCase subunit alpha
EC=6.4.1.4
Alternative name(s):
3-methylcrotonyl-CoA carboxylase 1
3-methylcrotonyl-CoA carboxylase biotin-containing subunit
3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha
Gene names
Name:Mccc1
Synonyms:Mcca
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length715 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA. UniProtKB Q96RQ3

Cofactor

Biotin By similarity. UniProtKB Q96RQ3

Pathway

Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.

Subunit structure

Probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits By similarity. UniProtKB Q96RQ3

Subcellular location

Mitochondrion matrix By similarity UniProtKB Q96RQ3.

Sequence similarities

Contains 1 ATP-grasp domain.

Contains 1 biotin carboxylation domain.

Contains 1 biotinyl-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3838Mitochondrion By similarity
Chain39 – 715677Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
PRO_0000365095

Regions

Domain45 – 490446Biotin carboxylation
Domain163 – 360198ATP-grasp
Domain625 – 71086Biotinyl-binding

Sites

Active site3351 By similarity UniProtKB P24182
Binding site1591ATP By similarity UniProtKB P24182
Binding site2431ATP By similarity UniProtKB P24182
Binding site2781ATP By similarity UniProtKB P24182

Amino acid modifications

Modified residue6771N6-biotinyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5I0C3 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 20FB2EAB8664AD8F

FASTA71579,330
        10         20         30         40         50         60 
MAAAALLAAV DRNQLRRVPI LLLQPREWPW KHRTVKYGTT PGGSITKVLI ANRGEIACRV 

        70         80         90        100        110        120 
IRTARKMGVQ SVAVYSEADR NSMHVDMADE AYSIGPAPSQ QSYLAMEKII QVAKSSAAQA 

       130        140        150        160        170        180 
IHPGYGFLSE NMEFAEFCKQ EGIIFIGPPS TAIRDMGIKS TSKSIMAAAG VPVVEGYHGN 

       190        200        210        220        230        240 
DQSDECLKEH AGKIGYPVMI KAIRGGGGKG MRIIRSEKEF QEQLESARRE AKKSFNDDAM 

       250        260        270        280        290        300 
LIEKFVDTPR HVEVQVFGDH HGNAVYLFER DCSVQRRHQK IIEEAPAPGI DPEVRRRLGE 

       310        320        330        340        350        360 
AAVRAAKAVN YVGAGTVEFI MDSKHNFYFM EMNTRLQVEH PVTEMITGTD LVEWQLRIAA 

       370        380        390        400        410        420 
GEKIPLSQEE IPLQGHAFEA RIYAEDPDNN FMPGAGPLVH LSTPPPDMST RIETGVRQGD 

       430        440        450        460        470        480 
EVSVHYDPMI AKLVVWASDR QSALSKLRYS LHQYNIVGLR TNVDFLLRLS GHSEFEAGNV 

       490        500        510        520        530        540 
HTDFIPQHHK DLLPTHSTIA KESVCQAALG LILKEKEMTS AFKLHTQDQF SPFSFSSGRR 

       550        560        570        580        590        600 
LNISYTRNMT LRSGKNDIII AVTYNRDGSY DMQIENKLFR VLGDLSNEDG YTYLKSSVNG 

       610        620        630        640        650        660 
VASKSKFILL DNTIYLFSME GSIEVGIPVP KYLSPVSAEG TQGGTIAPMT GTIEKVFVKA 

       670        680        690        700        710 
GDRVKAGDAL MVMIAMKMEH TIKAPKDGRI KKVFFSEGAQ ANRHAPLVEF EEEEV

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Brown Norway.
Tissue: Kidney.
[2]Maurya D.K., Bhargava P.
Submitted (JAN-2009) to UniProtKB
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC088473 mRNA. Translation: AAH88473.1.
IPIIPI00372191.
RefSeqNP_001009653.1. NM_001009653.1.
UniGeneRn.15587.

3D structure databases

HSSPHSSP built from PDB template 1BNC based on UniProtKB P24182.
ProteinModelPortalQ5I0C3.
SMRQ5I0C3. Positions 641-714.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5I0C3.

PTM databases

PhosphoSiteQ5I0C3.

Proteomic databases

PRIDEQ5I0C3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID294972.
KEGGrno:294972.
UCSCNM_001009653. rat.

Organism-specific databases

CTD56922.
RGD1310615. Mccc1.

Phylogenomic databases

eggNOGroNOG07266.
GeneTreeENSGT00550000074675.
HOVERGENHBG000555.
InParanoidQ5I0C3.
OrthoDBEOG4WQ123.

Gene expression databases

ArrayExpressQ5I0C3.
GenevestigatorQ5I0C3.

Family and domain databases

InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005479. CarbamoylP_synth_lsu_ATP-bd.
IPR005481. CarbamoylP_synth_lsu_N.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KOK01968.
PfamPF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMSSF51230. Hybrid_motif. 1 hit.
SSF52440. PreATP-grasp-like. 1 hit.
SSF51246. Rudmnt_hyb_motif. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. False negative.
PS00867. CPSASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio638797.

Entry information

Entry nameMCCA_RAT
AccessionPrimary (citable) accession number: Q5I0C3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: February 15, 2005
Last modified: November 16, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents