Reviewed, UniProtKB/Swiss-Prot Q5I0A0 (AMPM1_XENTR)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase 1
      Short name=MetAP 1
      Short name=MAP 1
    EC=3.4.11.18
Alternative name(s):
    Peptidase M 1

Gene names

Name:

metap1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity. Binds 1 sodium ion per subunit. The sodium ion has a structural role By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Methionine aminopeptidase 1		PRO_0000323739
Sites
	Metal binding	220	1	Cobalt 1 By similarity
	Metal binding	231	1	Cobalt 1 By similarity
	Metal binding	231	1	Cobalt 2 By similarity
	Metal binding	294	1	Cobalt 2 By similarity
	Metal binding	327	1	Cobalt 2 By similarity
	Metal binding	358	1	Cobalt 1 By similarity
	Metal binding	358	1	Cobalt 2 By similarity
	Binding site	203	1	Substrate By similarity
	Binding site	301	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5I0A0-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 4FDED5D82AA81873	FASTA	385	43,197
	10 20 30 40 50 60 MAAVESRVCE TEGCSSEAKL QCPTCIKLGI QGSYFCSQEC FKGSWASHKL LHKKAKDDKI 70 80 90 100 110 120 KPETSPWTMD GDINTDPWPG YRYTGKLRPH YPLTPMRPVP SYIQRPDYAD HPLGMSESEQ 130 140 150 160 170 180 ALKGTSQIKI LSTEDIEGMR VVCRLAREVL GVAAMMVKSG ITTEEIDHAV HLACISRNCY 190 200 210 220 230 240 PSPLNYYNFP KSCCTSVNEV ICHGIPDRRP LQDGDIVNVD ITVYRDGYHG DLNETFYVGD 250 260 270 280 290 300 VDEGAKRLVE TTYECLMQAI DEVKPGVRYR ELGNIIQKHA QANGFSVVRS YCGHGIHKLF 310 320 330 340 350 360 HTAPNVPHYA KNKAVGVMKP GHVFTIEPMI CEGGWQDETW PDGWTAITRD GKRSAQFEHT 370 380 LLVTETGCEI LTRRLEENGR PHFIS

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC088554 mRNA. Translation: AAH88554.1.
RefSeq	NP_001011454.1.
UniGene	Str.48028
3D structure databases
SMR	Q5I0A0. Positions 81-385.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5I0A0.
Protein family/group databases
MEROPS	M24.001.
Proteomic databases
PRIDE	Q5I0A0.
Genome annotation databases
GeneID	496944.
KEGG	xtr:496944.
Organism-specific databases
CTD	496944.
Xenbase	XB-FEAT-985732. metap1.
Phylogenomic databases
HOVERGEN	Q5I0A0.
Enzyme and pathway databases
BRENDA	3.4.11.18. 279072.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00500. met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM1_XENTR

Accession

Primary (citable) accession number: Q5I0A0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	February 15, 2005
Last modified:	September 1, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project