Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5I043 (UBP28_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 28

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 28
Ubiquitin thioesterase 28
Ubiquitin-specific-processing protease 28
Gene names
Name:Usp28
Synonyms:Kiaa1515
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1051 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBXW7) complex By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with FBXW7; following DNA damage, dissociates from FBXW7 leading to degradation of MYC.

Subcellular location

Nucleusnucleoplasm By similarity.

Post-translational modification

Degradaded upon nickel ion level or hypoxia exposure By similarity.

Phosphorylated upon DNA damage at Ser-67 and Ser-720, by ATM or ATR By similarity.

Sequence similarities

Belongs to the peptidase C19 family. USP28 subfamily.

Contains 1 UIM (ubiquitin-interacting motif) repeat.

Contains 1 USP domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
Ubl conjugation pathway
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   Molecular functionHydrolase
Protease
Thiol protease
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA damage checkpoint

Inferred from sequence or structural similarity. Source: UniProtKB

DNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to UV

Inferred from electronic annotation. Source: Ensembl

intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator

Inferred from sequence or structural similarity. Source: UniProtKB

protein deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

response to ionizing radiation

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleolus

Inferred from electronic annotation. Source: Ensembl

nucleoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

protein complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionubiquitin thiolesterase activity

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin-specific protease activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5I043-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5I043-2)

The sequence of this isoform differs from the canonical sequence as follows:
     429-453: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10511051Ubiquitin carboxyl-terminal hydrolase 28
PRO_0000080658

Regions

Repeat97 – 11620UIM
Domain162 – 655494USP

Sites

Active site1711Nucleophile By similarity
Active site6051Proton acceptor By similarity

Amino acid modifications

Modified residue671Phosphoserine By similarity
Modified residue7201Phosphoserine By similarity

Natural variations

Alternative sequence429 – 45325Missing in isoform 2.
VSP_015581

Experimental info

Sequence conflict5231P → L in BAC98190. Ref.3
Sequence conflict6171T → I in BAC98190. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 72623C4668860749

FASTA1,051119,318
        10         20         30         40         50         60 
MTAELQQDDS AGAADGHGSS CQMLLNQLRE ITGIQDPSFL HEALKASNGD ITQAVSLLTD 

        70         80         90        100        110        120 
QRVKEPSHDT TAAEPSEVEE SATSKDLLAK VIDLTHDNKD DLQAAIALSL LESPNIQADN 

       130        140        150        160        170        180 
RDLNRAHEAN SAETKRSKRK RCEVWGENHN PNNWRRVDGW PVGLKNVGNT CWFSAVIQSL 

       190        200        210        220        230        240 
FQLPEFRRLV LSYNLPQNIL ENCRSHTEKR NIMFMQELQY LFALLLGSNR KFVDPSAALD 

       250        260        270        280        290        300 
LLKGAFRSSE EQQQDVSEFT HKLLDWLEDA FQLAVNVNSH LRNKSENPMV QLFYGTFLTE 

       310        320        330        340        350        360 
GVREGKPFCN NETFGQYPLQ VNGYHNLDEC LEGAMVEGDI ALLPSDRSVK YGQERWFTKL 

       370        380        390        400        410        420 
PPVLTFELSR FEFNQSLGQP EKIHNKLEFP QIIYMDRYMY KSKELIRSKR ESVRKLKEEI 

       430        440        450        460        470        480 
QVLQQKLERY VKYGSGPSRF PLPDMLKYVI EFASTKPASE SCLSGSAEHV TLPLPSVHCP 

       490        500        510        520        530        540 
ISDLTPKESS SPESCSQNAG STFSSPEDAL PSSEGMNGPF TSPHSSLETP APPAPRTVTD 

       550        560        570        580        590        600 
EEMNFVKTCL QRWRSEIEQD IQDLKNCISS STKAIEQMYC DPLLRQVPYR LHAVLVHEGQ 

       610        620        630        640        650        660 
ASAGHYWAYI YNQPRQTWLK YNDISVTESS WEELERDSYG GLRNVSAYCL MYINDNLPHF 

       670        680        690        700        710        720 
SAEASSNESD ETAGEVEALS VELRQYIQED NWRFQQEVEE WEEEQSCKIP QMESSPNSSS 

       730        740        750        760        770        780 
QDFSTSQESP AVSSHEVRCL SSEHAVIAKE QTAQAIANTA HAYEKSGVEA ALSEAFHEEY 

       790        800        810        820        830        840 
SRLYQLAKET PTSHSDPRLQ HVLVYFFQNE APKRVVERTL LEQFADRNLS YDERSISIMK 

       850        860        870        880        890        900 
VAQAKLMEIG PDDMNMEEYK RWHEDYSLFR KVSVYLLTGL ELFQKGKYQE ALSYLVYAYQ 

       910        920        930        940        950        960 
SNAGLLVKGP RRGVKESVIA LYRRKCLLEL NAKAASLFET NDDHSVTEGI NVMNELIIPC 

       970        980        990       1000       1010       1020 
IHLIINNDIS KDDLDAIEVM RNHWCSYLGK DIAENLQLCL GEFLPRLLDP SAEIIVLKEP 

      1030       1040       1050 
PTIRPNSPYD LCNRFAAVME SIQGVSTVTV K 

« Hide

Isoform 2 [UniParc].

Checksum: 4620DBD05FE6E55A
Show »

FASTA1,026116,402

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Brain and Embryonic germ cell.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 292-1051.
Strain: C57BL/6J.
Tissue: Lung.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 392-1051.
Tissue: Embryonic tail.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC066033 mRNA. Translation: AAH66033.1.
BC088733 mRNA. Translation: AAH88733.1.
AK052442 mRNA. Translation: BAC34993.1.
AK129380 mRNA. Translation: BAC98190.1.
RefSeqNP_780691.2. NM_175482.3.
UniGeneMm.21630.

3D structure databases

ProteinModelPortalQ5I043.
SMRQ5I043. Positions 22-132, 162-402, 576-656.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC19.054.

PTM databases

PhosphoSiteQ5I043.

Proteomic databases

PaxDbQ5I043.
PRIDEQ5I043.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000047349; ENSMUSP00000047467; ENSMUSG00000032267. [Q5I043-1]
GeneID235323.
KEGGmmu:235323.
UCSCuc009pir.1. mouse. [Q5I043-1]
uc009pis.1. mouse. [Q5I043-2]

Organism-specific databases

CTD57646.
MGIMGI:2442293. Usp28.
RougeSearch...

Phylogenomic databases

eggNOGCOG5077.
GeneTreeENSGT00390000016082.
HOGENOMHOG000007956.
HOVERGENHBG056030.
InParanoidQ5I043.
KOK11849.
OMACIASTTQ.
OrthoDBEOG761BSZ.
PhylomeDBQ5I043.
TreeFamTF329035.

Gene expression databases

ArrayExpressQ5I043.
BgeeQ5I043.
CleanExMM_USP28.
GenevestigatorQ5I043.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR003903. Ubiquitin-int_motif.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
SMARTSM00726. UIM. 1 hit.
[Graphical view]
SUPFAMSSF46934. SSF46934. 1 hit.
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio382599.
PROQ5I043.
SOURCESearch...

Entry information

Entry nameUBP28_MOUSE
AccessionPrimary (citable) accession number: Q5I043
Secondary accession number(s): Q6NZP3, Q6ZPP1, Q8BWI1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: February 15, 2005
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot