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Q5I043

- UBP28_MOUSE

UniProt

Q5I043 - UBP28_MOUSE

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Protein
Ubiquitin carboxyl-terminal hydrolase 28
Gene
Usp28, Kiaa1515
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBXW7) complex By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei171 – 1711Nucleophile By similarity
Active sitei605 – 6051Proton acceptor By similarity

GO - Molecular functioni

  1. ubiquitin thiolesterase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. DNA damage checkpoint Source: UniProtKB
  2. DNA repair Source: UniProtKB-KW
  3. cell proliferation Source: UniProtKB
  4. cellular response to UV Source: Ensembl
  5. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: UniProtKB
  6. protein deubiquitination Source: UniProtKB
  7. response to ionizing radiation Source: UniProtKB
  8. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.054.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 28 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 28
Ubiquitin thioesterase 28
Ubiquitin-specific-processing protease 28
Gene namesi
Name:Usp28
Synonyms:Kiaa1515
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:2442293. Usp28.

Subcellular locationi

Nucleusnucleoplasm By similarity

GO - Cellular componenti

  1. nucleolus Source: Ensembl
  2. nucleoplasm Source: UniProtKB
  3. protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10511051Ubiquitin carboxyl-terminal hydrolase 28
PRO_0000080658Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671Phosphoserine By similarity
Modified residuei720 – 7201Phosphoserine By similarity

Post-translational modificationi

Degradaded upon nickel ion level or hypoxia exposure By similarity.
Phosphorylated upon DNA damage at Ser-67 and Ser-720, by ATM or ATR By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ5I043.
PaxDbiQ5I043.
PRIDEiQ5I043.

PTM databases

PhosphoSiteiQ5I043.

Expressioni

Gene expression databases

ArrayExpressiQ5I043.
BgeeiQ5I043.
CleanExiMM_USP28.
GenevestigatoriQ5I043.

Interactioni

Subunit structurei

Interacts with FBXW7; following DNA damage, dissociates from FBXW7 leading to degradation of MYC.

Structurei

3D structure databases

ProteinModelPortaliQ5I043.
SMRiQ5I043. Positions 22-132, 162-402, 576-656.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati97 – 11620UIM
Add
BLAST
Domaini162 – 655494USP
Add
BLAST

Sequence similaritiesi

Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00390000016082.
HOGENOMiHOG000007956.
HOVERGENiHBG056030.
InParanoidiQ5I043.
KOiK11849.
OMAiCIASTTQ.
OrthoDBiEOG761BSZ.
PhylomeDBiQ5I043.
TreeFamiTF329035.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR003903. Ubiquitin-int_motif.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00726. UIM. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q5I043-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTAELQQDDS AGAADGHGSS CQMLLNQLRE ITGIQDPSFL HEALKASNGD     50
ITQAVSLLTD QRVKEPSHDT TAAEPSEVEE SATSKDLLAK VIDLTHDNKD 100
DLQAAIALSL LESPNIQADN RDLNRAHEAN SAETKRSKRK RCEVWGENHN 150
PNNWRRVDGW PVGLKNVGNT CWFSAVIQSL FQLPEFRRLV LSYNLPQNIL 200
ENCRSHTEKR NIMFMQELQY LFALLLGSNR KFVDPSAALD LLKGAFRSSE 250
EQQQDVSEFT HKLLDWLEDA FQLAVNVNSH LRNKSENPMV QLFYGTFLTE 300
GVREGKPFCN NETFGQYPLQ VNGYHNLDEC LEGAMVEGDI ALLPSDRSVK 350
YGQERWFTKL PPVLTFELSR FEFNQSLGQP EKIHNKLEFP QIIYMDRYMY 400
KSKELIRSKR ESVRKLKEEI QVLQQKLERY VKYGSGPSRF PLPDMLKYVI 450
EFASTKPASE SCLSGSAEHV TLPLPSVHCP ISDLTPKESS SPESCSQNAG 500
STFSSPEDAL PSSEGMNGPF TSPHSSLETP APPAPRTVTD EEMNFVKTCL 550
QRWRSEIEQD IQDLKNCISS STKAIEQMYC DPLLRQVPYR LHAVLVHEGQ 600
ASAGHYWAYI YNQPRQTWLK YNDISVTESS WEELERDSYG GLRNVSAYCL 650
MYINDNLPHF SAEASSNESD ETAGEVEALS VELRQYIQED NWRFQQEVEE 700
WEEEQSCKIP QMESSPNSSS QDFSTSQESP AVSSHEVRCL SSEHAVIAKE 750
QTAQAIANTA HAYEKSGVEA ALSEAFHEEY SRLYQLAKET PTSHSDPRLQ 800
HVLVYFFQNE APKRVVERTL LEQFADRNLS YDERSISIMK VAQAKLMEIG 850
PDDMNMEEYK RWHEDYSLFR KVSVYLLTGL ELFQKGKYQE ALSYLVYAYQ 900
SNAGLLVKGP RRGVKESVIA LYRRKCLLEL NAKAASLFET NDDHSVTEGI 950
NVMNELIIPC IHLIINNDIS KDDLDAIEVM RNHWCSYLGK DIAENLQLCL 1000
GEFLPRLLDP SAEIIVLKEP PTIRPNSPYD LCNRFAAVME SIQGVSTVTV 1050
K 1051
Length:1,051
Mass (Da):119,318
Last modified:February 15, 2005 - v1
Checksum:i72623C4668860749
GO
Isoform 2 (identifier: Q5I043-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     429-453: Missing.

Note: No experimental confirmation available.

Show »
Length:1,026
Mass (Da):116,402
Checksum:i4620DBD05FE6E55A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei429 – 45325Missing in isoform 2.
VSP_015581Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti523 – 5231P → L in BAC98190. 1 Publication
Sequence conflicti617 – 6171T → I in BAC98190. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC066033 mRNA. Translation: AAH66033.1.
BC088733 mRNA. Translation: AAH88733.1.
AK052442 mRNA. Translation: BAC34993.1.
AK129380 mRNA. Translation: BAC98190.1.
CCDSiCCDS40613.1. [Q5I043-1]
RefSeqiNP_780691.2. NM_175482.3. [Q5I043-1]
UniGeneiMm.21630.

Genome annotation databases

EnsembliENSMUST00000047349; ENSMUSP00000047467; ENSMUSG00000032267. [Q5I043-1]
GeneIDi235323.
KEGGimmu:235323.
UCSCiuc009pir.1. mouse. [Q5I043-1]
uc009pis.1. mouse. [Q5I043-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC066033 mRNA. Translation: AAH66033.1 .
BC088733 mRNA. Translation: AAH88733.1 .
AK052442 mRNA. Translation: BAC34993.1 .
AK129380 mRNA. Translation: BAC98190.1 .
CCDSi CCDS40613.1. [Q5I043-1 ]
RefSeqi NP_780691.2. NM_175482.3. [Q5I043-1 ]
UniGenei Mm.21630.

3D structure databases

ProteinModelPortali Q5I043.
SMRi Q5I043. Positions 22-132, 162-402, 576-656.
ModBasei Search...

Protein family/group databases

MEROPSi C19.054.

PTM databases

PhosphoSitei Q5I043.

Proteomic databases

MaxQBi Q5I043.
PaxDbi Q5I043.
PRIDEi Q5I043.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047349 ; ENSMUSP00000047467 ; ENSMUSG00000032267 . [Q5I043-1 ]
GeneIDi 235323.
KEGGi mmu:235323.
UCSCi uc009pir.1. mouse. [Q5I043-1 ]
uc009pis.1. mouse. [Q5I043-2 ]

Organism-specific databases

CTDi 57646.
MGIi MGI:2442293. Usp28.
Rougei Search...

Phylogenomic databases

eggNOGi COG5077.
GeneTreei ENSGT00390000016082.
HOGENOMi HOG000007956.
HOVERGENi HBG056030.
InParanoidi Q5I043.
KOi K11849.
OMAi CIASTTQ.
OrthoDBi EOG761BSZ.
PhylomeDBi Q5I043.
TreeFami TF329035.

Miscellaneous databases

NextBioi 382599.
PROi Q5I043.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q5I043.
Bgeei Q5I043.
CleanExi MM_USP28.
Genevestigatori Q5I043.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR003903. Ubiquitin-int_motif.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00726. UIM. 1 hit.
[Graphical view ]
SUPFAMi SSF46934. SSF46934. 1 hit.
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Brain and Embryonic germ cell.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 292-1051.
    Strain: C57BL/6J.
    Tissue: Lung.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 392-1051.
    Tissue: Embryonic tail.

Entry informationi

Entry nameiUBP28_MOUSE
AccessioniPrimary (citable) accession number: Q5I043
Secondary accession number(s): Q6NZP3, Q6ZPP1, Q8BWI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: February 15, 2005
Last modified: July 9, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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