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Q5HZJ0

- RNC_MOUSE

UniProt

Q5HZJ0 - RNC_MOUSE

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Protein

Ribonuclease 3

Gene

Drosha

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Magnesium or manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1146 – 11461Magnesium or manganeseBy similarity
Sitei1214 – 12141Important for activityBy similarity
Metal bindingi1218 – 12181Magnesium or manganeseBy similarity
Metal bindingi1221 – 12211Magnesium or manganeseBy similarity

GO - Molecular functioni

  1. endoribonuclease activity Source: MGI
  2. lipopolysaccharide binding Source: Ensembl
  3. metal ion binding Source: UniProtKB-KW
  4. poly(A) RNA binding Source: Ensembl
  5. ribonuclease III activity Source: UniProtKB-EC

GO - Biological processi

  1. defense response to Gram-negative bacterium Source: Ensembl
  2. defense response to Gram-positive bacterium Source: Ensembl
  3. miRNA metabolic process Source: MGI
  4. pre-miRNA processing Source: MGI
  5. primary miRNA processing Source: Ensembl
  6. regulation of gene expression Source: MGI
  7. regulation of miRNA metabolic process Source: MGI
  8. ribosome biogenesis Source: UniProtKB-KW
  9. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  10. rRNA catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, RNA-binding

Enzyme and pathway databases

ReactomeiREACT_221178. MicroRNA (miRNA) biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Protein Drosha
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:Drosha
Synonyms:Etohi2, Rn3, Rnasen
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1261425. Drosha.

Subcellular locationi

Nucleus By similarity. Nucleusnucleolus By similarity
Note: A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies (By similarity).By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13731373Ribonuclease 3PRO_0000384374Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei372 – 3721PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ5HZJ0.
PaxDbiQ5HZJ0.
PRIDEiQ5HZJ0.

PTM databases

PhosphoSiteiQ5HZJ0.

Expressioni

Gene expression databases

BgeeiQ5HZJ0.
ExpressionAtlasiQ5HZJ0. baseline and differential.
GenevestigatoriQ5HZJ0.

Interactioni

Subunit structurei

Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8. The microprocessor complex may contain multiple subunit of DGCR8 and DROSHA. Interacts with DGCR8, SP1 and SNIP1 (By similarity). Interacts with SRRT/ARS2.By similarity1 Publication

Protein-protein interaction databases

BioGridi199531. 1 interaction.
IntActiQ5HZJ0. 1 interaction.
STRINGi10090.ENSMUSP00000087762.

Structurei

3D structure databases

ProteinModelPortaliQ5HZJ0.
SMRiQ5HZJ0. Positions 961-1336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini875 – 1055181RNase III 1Add
BLAST
Domaini1106 – 1232127RNase III 2Add
BLAST
Domaini1259 – 133375DRBMAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni489 – 1373885Necessary for interaction with DGCR8 and pri-miRNA processing activityBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi28 – 211184Pro-richAdd
BLAST
Compositional biasi217 – 31599Arg-richAdd
BLAST

Domaini

The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.By similarity

Sequence similaritiesi

Contains 2 RNase III domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0571.
GeneTreeiENSGT00730000111052.
HOGENOMiHOG000122291.
InParanoidiQ5HZJ0.
KOiK03685.
OMAiESYYSND.
OrthoDBiEOG773XF7.
PhylomeDBiQ5HZJ0.
TreeFamiTF314734.

Family and domain databases

Gene3Di1.10.1520.10. 3 hits.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 3 hits.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 2 hits.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HZJ0 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQGNTCHRMS YHPGRGCPRG RGGHGARPSA PAFRPQNLRL LHPQQPPAQY
60 70 80 90 100
QYEPPSAPSS SYSNSQAPSF MPPRPDFVPY PPPAAPSAQG PLPPCPVRPP
110 120 130 140 150
YPNHQMRHPF PVPPCFPPMP PPMPCPNNPP ASGAPPGQGT FPFMVPPPSM
160 170 180 190 200
PHPPPPPVMP QQVNYQYPPG YSHSFPPPGF NSYQNNSSSF PPSANSSSTP
210 220 230 240 250
HFRHLPPYSL PKAQNERRSP ERLKHYDDHR HRDHSHGRGE RHRSLERRER
260 270 280 290 300
GRSPERRRPE SRYRSDYDRG RTPPPRHRSY ERSRERDRER HRHREARRSP
310 320 330 340 350
SLERSYKKEY KRSGRSYALP VAPEPAGCTP ELPGEMIKTT ESWAPPPENV
360 370 380 390 400
NHRSPSREKK RARWEEEKDR WSDSQGSGKE KNYTSIKEKE AEEVPPEKTE
410 420 430 440 450
EEEEELLKPV WIRCTHSESY YSSDPMDQVG DSTVVGTSRL RDLYDKFEEE
460 470 480 490 500
LGNRQEKAKA ARPPWEPPKT KLDEDLESSS ESECETDDDS TCSSSSDSEV
510 520 530 540 550
FDVIAEIKRK KAHPDRLHDE LWYNDPGQMN DGPLCKCSAK ARRTGIRHSI
560 570 580 590 600
YPGEEAIKPC RPMTNNAGRL FHYRITVSPP TNFLTDRPTV IEYDDHEYIF
610 620 630 640 650
EGFSMFAHAP LTNIPLCKVI RFNIDYTIHF IEEMMPENFC VKGLELFSLF
660 670 680 690 700
LFRDILELYD WNLKGPLFED SPPCCPRFHF MPRFVRFLPD GGKEVLSMHQ
710 720 730 740 750
ILLYLLRCSK ALVPEEEIAN MLQWEELEWQ KYAEECKGMI VTNPGTKPSS
760 770 780 790 800
VRIDQLDREQ FNPEVITFPI IVHFGIRPAQ LSYAGDPQYQ KLWKSYVKLR
810 820 830 840 850
HLLANSPKVK QTDKQKLAQR EEALQKIRQK NTMRREVTVE LSSQGFWKTG
860 870 880 890 900
IRSDVCQHAM MLPVLTHHIR YHQCLMHLDK LIGYTFQDRC LLQLAMTHPS
910 920 930 940 950
HHLNFGMNPD HARNSLSNCG IRQPKYGDRK VHHMHMRKKG INTLINIMSR
960 970 980 990 1000
LGQDDPTPSR INHNERLEFL GDAVVEFLTS VHLYYLFPSL EEGGLATYRT
1010 1020 1030 1040 1050
AIVQNQHLAM LAKKLELDRF MLYAHGPDLC RESDLRHAMA NCFEALIGAV
1060 1070 1080 1090 1100
YLEGSLEEAK QLFGRLLFND PDLREVWLNY PLHPLQLQEP NTDRQLIETS
1110 1120 1130 1140 1150
PVLQKLTEFE EAIGVIFTHV RLLARAFTLR TVGFNHLTLG HNQRMEFLGD
1160 1170 1180 1190 1200
SIMQLVATEY LFIHFPDHHE GHLTLLRSSL VNNRTQAKVA EELGMQEYAI
1210 1220 1230 1240 1250
TNDKTKRPVA LRTKTLADLL ESFIAALYID KDLEYVHTFM NVCFFPRLKE
1260 1270 1280 1290 1300
FILNQDWNDP KSQLQQCCLT LRTEGKEPDI PLYKTLQTVG PSHARTYTVA
1310 1320 1330 1340 1350
VYFKGERIGC GKGPSIQQAE MGAAMDALEK YNFPQMAHQK RFIERKYRQE
1360 1370
LKEMRWEREH QEREPEEAED IKK
Length:1,373
Mass (Da):158,828
Last modified:February 15, 2005 - v1
Checksum:iED1DBEE2FFD89A6B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK144147 mRNA. Translation: BAE25729.1.
AK148640 mRNA. Translation: BAE28629.1.
BC088999 mRNA. Translation: AAH88999.1.
CCDSiCCDS49583.1.
RefSeqiNP_001123621.1. NM_001130149.1.
NP_081075.3. NM_026799.3.
UniGeneiMm.293142.

Genome annotation databases

EnsembliENSMUST00000090292; ENSMUSP00000087762; ENSMUSG00000022191.
ENSMUST00000169061; ENSMUSP00000129279; ENSMUSG00000022191.
GeneIDi14000.
KEGGimmu:14000.
UCSCiuc007via.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK144147 mRNA. Translation: BAE25729.1 .
AK148640 mRNA. Translation: BAE28629.1 .
BC088999 mRNA. Translation: AAH88999.1 .
CCDSi CCDS49583.1.
RefSeqi NP_001123621.1. NM_001130149.1.
NP_081075.3. NM_026799.3.
UniGenei Mm.293142.

3D structure databases

ProteinModelPortali Q5HZJ0.
SMRi Q5HZJ0. Positions 961-1336.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199531. 1 interaction.
IntActi Q5HZJ0. 1 interaction.
STRINGi 10090.ENSMUSP00000087762.

PTM databases

PhosphoSitei Q5HZJ0.

Proteomic databases

MaxQBi Q5HZJ0.
PaxDbi Q5HZJ0.
PRIDEi Q5HZJ0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000090292 ; ENSMUSP00000087762 ; ENSMUSG00000022191 .
ENSMUST00000169061 ; ENSMUSP00000129279 ; ENSMUSG00000022191 .
GeneIDi 14000.
KEGGi mmu:14000.
UCSCi uc007via.2. mouse.

Organism-specific databases

CTDi 29102.
MGIi MGI:1261425. Drosha.

Phylogenomic databases

eggNOGi COG0571.
GeneTreei ENSGT00730000111052.
HOGENOMi HOG000122291.
InParanoidi Q5HZJ0.
KOi K03685.
OMAi ESYYSND.
OrthoDBi EOG773XF7.
PhylomeDBi Q5HZJ0.
TreeFami TF314734.

Enzyme and pathway databases

Reactomei REACT_221178. MicroRNA (miRNA) biogenesis.

Miscellaneous databases

ChiTaRSi DROSHA. mouse.
NextBioi 284892.
PROi Q5HZJ0.
SOURCEi Search...

Gene expression databases

Bgeei Q5HZJ0.
ExpressionAtlasi Q5HZJ0. baseline and differential.
Genevestigatori Q5HZJ0.

Family and domain databases

Gene3Di 1.10.1520.10. 3 hits.
3.30.160.20. 1 hit.
HAMAPi MF_00104. RNase_III.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 3 hits.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 2 hits.
PS50142. RNASE_3_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryonic stem cell and Sympathetic ganglion.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Embryonic brain.
  3. "Ars2 links the nuclear cap-binding complex to RNA interference and cell proliferation."
    Gruber J.J., Zatechka D.S., Sabin L.R., Yong J., Lum J.J., Kong M., Zong W.-X., Zhang Z., Lau C.-K., Rawlings J., Cherry S., Ihle J.N., Dreyfuss G., Thompson C.B.
    Cell 138:328-339(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRRT.

Entry informationi

Entry nameiRNC_MOUSE
AccessioniPrimary (citable) accession number: Q5HZJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 15, 2005
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3