Reviewed, UniProtKB/Swiss-Prot Q5HZ60 (MCES2_ARATH)

Names and origin

Protein names

Recommended name:
    mRNA cap guanine-N7 methyltransferase 2
    EC=2.1.1.56
Alternative name(s):
    mRNA (guanine-N(7)-)-methyltransferase 2
    mRNA cap methyltransferase 2

Gene names

Ordered Locus Names:	At3g52210
ORF Names:	F4F15.320, T25B15.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	mRNA capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC By similarity.
Catalytic activity	S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the mRNA cap methyltransferase family.
Sequence caution	The sequence CAB41341.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5HZ60-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5HZ60-2)

The sequence of this isoform differs from the canonical sequence as follows:
     60-60: I → IV

Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Isoform 3 (identifier: Q5HZ60-3)

The sequence of this isoform differs from the canonical sequence as follows:
     246-250: RAQFA → SLQAC
     251-354: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	mRNA cap guanine-N7 methyltransferase 2		PRO_0000260152
Sites
	Binding site	21	1	S-adenosyl-L-methionine By similarity
	Binding site	48	1	mRNA cap By similarity
	Binding site	61	1	S-adenosyl-L-methionine By similarity
	Binding site	278	1	mRNA cap By similarity
Natural variations
	Alternative sequence	60	1	I → IV in isoform 2.		VSP_021576
	Alternative sequence	246 – 250	5	RAQFA → SLQAC in isoform 3.		VSP_021577
	Alternative sequence	251 – 354	104	Missing in isoform 3.		VSP_021578
Experimental info
	Sequence conflict	83	1	E → G in BAD43168. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: D6950C807B3966D5	FASTA	354	39,889
	10 20 30 40 50 60 MSGFVVSKPE QSHHRLFDFA KTAIINIFAH PYATVCELYC GGAPETDKWE AAPIGHYIGI 70 80 90 100 110 120 DTSSGISSVR EAWESQRKNY DVEFFEADPS KDDFEIQLQK KLEQADLVSC WRHLQLCFET 130 140 150 160 170 180 EESARRLLTN VACLLKPGGY FFGITPDSST IWAKYQKNVE AYHNRSGAKP NVFPNYIRSE 190 200 210 220 230 240 SYMITFELEE EKFPLFGKRY QLKFSGDNAS EDHCLVHFPS LIRLAREAGL EFVEIQSLTD 250 260 270 280 290 300 FYDDNRAQFA SLLMNAGPNF VDPRGKLLPR AFDLLGLYAT FIFQKPDPDI EPPLTTPIPF 310 320 330 340 350 ESSNNHDERE LPVITVITDA SAPAEDPSQG LGKIVEQKGI LGPGPADLRF SEAI
	Isoform 2. Checksum: 480C5A4DE96E07A5	FASTA	355	39,988
	10 20 30 40 50 60 MSGFVVSKPE QSHHRLFDFA KTAIINIFAH PYATVCELYC GGAPETDKWE AAPIGHYIGI 70 80 90 100 110 120 VDTSSGISSV REAWESQRKN YDVEFFEADP SKDDFEIQLQ KKLEQADLVS CWRHLQLCFE 130 140 150 160 170 180 TEESARRLLT NVACLLKPGG YFFGITPDSS TIWAKYQKNV EAYHNRSGAK PNVFPNYIRS 190 200 210 220 230 240 ESYMITFELE EEKFPLFGKR YQLKFSGDNA SEDHCLVHFP SLIRLAREAG LEFVEIQSLT 250 260 270 280 290 300 DFYDDNRAQF ASLLMNAGPN FVDPRGKLLP RAFDLLGLYA TFIFQKPDPD IEPPLTTPIP 310 320 330 340 350 FESSNNHDER ELPVITVITD ASAPAEDPSQ GLGKIVEQKG ILGPGPADLR FSEAI
	Isoform 3. Checksum: 0367C8B0DF71AEEC	FASTA	250	28,628
	10 20 30 40 50 60 MSGFVVSKPE QSHHRLFDFA KTAIINIFAH PYATVCELYC GGAPETDKWE AAPIGHYIGI 70 80 90 100 110 120 DTSSGISSVR EAWESQRKNY DVEFFEADPS KDDFEIQLQK KLEQADLVSC WRHLQLCFET 130 140 150 160 170 180 EESARRLLTN VACLLKPGGY FFGITPDSST IWAKYQKNVE AYHNRSGAKP NVFPNYIRSE 190 200 210 220 230 240 SYMITFELEE EKFPLFGKRY QLKFSGDNAS EDHCLVHFPS LIRLAREAGL EFVEIQSLTD 250 FYDDNSLQAC

References

[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL049711 Genomic DNA. Translation: CAB41341.1. Sequence problems. AL132972 Genomic DNA. No translation available. AK175405 mRNA. Translation: BAD43168.1. AK176609 mRNA. Translation: BAD44372.1. AK176627 mRNA. Translation: BAD44390.1. BT020464 mRNA. Translation: AAW38965.1. BT020571 mRNA. Translation: AAW78590.1.
IPI	IPI00545771. IPI00656919. IPI00657101.
PIR	T49100.
RefSeq	NP_001030844.1. NP_190789.3.
UniGene	At.68448
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	824386.
GenomeReviews	Gene locus AT3G52210 in contig BA000014_GR.
NMPDR	fig|3702.1.peg.16486.
Organism-specific databases
GeneFarm	4527. 451.
TAIR	At3g52210.
Phylogenomic databases
OMA	Q5HZ60. YVEIQNL.
Enzyme and pathway databases
BRENDA	2.1.1.56. 302.
Family and domain databases
InterPro	IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF03291. Pox_MCEL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCES2_ARATH

Accession

Primary (citable) accession number: Q5HZ60
Secondary accession number(s): Q67Y41, Q682G2, Q9SUY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	February 15, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)