Q5HYJ3 (FA76B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein FAM76B

Gene names

Name:

FAM76B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus speckle Ref.8.
Domain	The polyhistidine repeat acts as a targeting signal to nuclear speckles (Ref.8).
Post-translational modification	Isoform 2 is ubiquitinated at Lys-225.
Sequence similarities	Belongs to the FAM76 family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5HYJ3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5HYJ3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     231-233: SSS → RSM
     234-339: Missing.

Note: Ubiquitinated at position 225.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Protein FAM76B		PRO_0000245763
Regions
	Coiled coil	248 – 328	81	Potential
	Compositional bias	151 – 160	10	Poly-Ser
	Compositional bias	167 – 187	21	His-rich
	Compositional bias	171 – 180	10	Poly-His
Amino acid modifications
	Modified residue	22	1	Phosphoserine Ref.6
	Modified residue	148	1	Phosphoserine Ref.10
	Modified residue	193	1	Phosphoserine Ref.4 Ref.7 Ref.9 Ref.10 Ref.12
	Cross-link	225		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5
Natural variations
	Alternative sequence	231 – 233	3	SSS → RSM in isoform 2.		VSP_022679
	Alternative sequence	234 – 339	106	Missing in isoform 2.		VSP_022680
Experimental info
	Sequence conflict	228	1	N → K in CAI46088. Ref.1
	Sequence conflict	328	1	K → E in CAI46088. Ref.1
	Sequence conflict	333	1	G → R in CAI46088. Ref.1
Isoform 2:
	Sequence conflict	232	1	S → C in AAH26013. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 3. Checksum: 4ED7FEE05F23790C	FASTA	339	38,708
	10 20 30 40 50 60 MAASALYACT KCTQRYPFEE LSQGQQLCKE CRIAHPIVKC TYCRSEFQQE SKTNTICKKC 70 80 90 100 110 120 AQNVKQFGTP KPCQYCNIIA AFIGTKCQRC TNSEKKYGPP QTCEQCKQQC AFDRKEEGRR 130 140 150 160 170 180 KVDGKLLCWL CTLSYKRVLQ KTKEQRKSLG SSHSNSSSSS LTEKDQHHPK HHHHHHHHHH 190 200 210 220 230 240 RHSSSHHKIS NLSPEEEQGL WKQSHKSSAT IQNETPKKKP KLESKPSNGD SSSINQSADS 250 260 270 280 290 300 GGTDNFVLIS QLKEEVMSLK RLLQQRDQTI LEKDKKLTEL KADFQYQESN LRTKMNSMEK 310 320 330 AHKETVEQLQ AKNRELLKQV AALSKGKKFD KSGSILTSP
	Isoform 2 [UniParc]. Checksum: 3080A28B11A7C341	FASTA	233	26,771
	10 20 30 40 50 60 MAASALYACT KCTQRYPFEE LSQGQQLCKE CRIAHPIVKC TYCRSEFQQE SKTNTICKKC 70 80 90 100 110 120 AQNVKQFGTP KPCQYCNIIA AFIGTKCQRC TNSEKKYGPP QTCEQCKQQC AFDRKEEGRR 130 140 150 160 170 180 KVDGKLLCWL CTLSYKRVLQ KTKEQRKSLG SSHSNSSSSS LTEKDQHHPK HHHHHHHHHH 190 200 210 220 230 RHSSSHHKIS NLSPEEEQGL WKQSHKSSAT IQNETPKKKP KLESKPSNGD RSM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Fetal skin.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells." Vasilescu J., Zweitzig D.R., Denis N.J., Smith J.C., Ethier M., Haines D.S., Figeys D. J. Proteome Res. 6:298-305(2007) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-225 (ISOFORM 2), MASS SPECTROMETRY. Tissue: Lung adenocarcinoma.
[6]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Genome-wide analysis of histidine repeats reveals their role in the localization of human proteins to the nuclear speckles compartment." Salichs E., Ledda A., Mularoni L., Alba M.M., de la Luna S. PLoS Genet. 5:E1000397-E1000397(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148 AND SER-193, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX647586 mRNA. Translation: CAI46088.1. AP001877 Genomic DNA. No translation available. BC026013 mRNA. Translation: AAH26013.1. BC028727 mRNA. Translation: AAH28727.1.
IPI	IPI00552849. IPI00827638.
RefSeq	NP_653265.3. NM_144664.4.
UniGene	Hs.288304.
3D structure databases
ProteinModelPortal	Q5HYJ3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5HYJ3. 1 interaction.
MINT	MINT-1472951.
STRING	9606.ENSP00000351631.
PTM databases
PhosphoSite	Q5HYJ3.
Polymorphism databases
DMDM	296439347.
Proteomic databases
PaxDb	Q5HYJ3.
PRIDE	Q5HYJ3.
Protocols and materials databases
DNASU	143684.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000358780; ENSP00000351631; ENSG00000077458.
GeneID	143684.
KEGG	hsa:143684.
UCSC	uc001pfn.2. human.
Organism-specific databases
CTD	143684.
GeneCards	GC11M095502.
H-InvDB	HIX0010043.
HGNC	HGNC:28492. FAM76B.
neXtProt	NX_Q5HYJ3.
PharmGKB	PA142671838.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG330390.
HOGENOM	HOG000231316.
HOVERGEN	HBG080224.
InParanoid	Q5HYJ3.
OMA	NQRYPFE.
OrthoDB	EOG4N04FF.
Gene expression databases
ArrayExpress	Q5HYJ3.
Bgee	Q5HYJ3.
CleanEx	HS_FAM76B.
Genevestigator	Q5HYJ3.
GermOnline	ENSG00000077458. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
GenomeRNAi	143684.
NextBio	84772.

Entry information

Entry name

FA76B_HUMAN

Accession

Primary (citable) accession number: Q5HYJ3
Secondary accession number(s): Q6PIU3, Q8TC53

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	May 18, 2010
Last modified:	April 3, 2013
This is version 65 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.