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Q5HYJ3

- FA76B_HUMAN

UniProt

Q5HYJ3 - FA76B_HUMAN

Protein

Protein FAM76B

Gene

FAM76B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 3 (18 May 2010)
      Previous versions | rss
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    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein FAM76B
    Gene namesi
    Name:FAM76B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:28492. FAM76B.

    Subcellular locationi

    Nucleus speckle 1 Publication

    GO - Cellular componenti

    1. nuclear membrane Source: HPA
    2. nuclear speck Source: UniProtKB-SubCell
    3. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671838.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed3 Publications
    Chaini2 – 339338Protein FAM76BPRO_0000245763Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine3 Publications
    Modified residuei22 – 221Phosphoserine1 Publication
    Modified residuei148 – 1481Phosphoserine1 Publication
    Modified residuei193 – 1931Phosphoserine5 Publications
    Cross-linki225 – 225Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

    Post-translational modificationi

    Isoform 2 is ubiquitinated at Lys-225.

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ5HYJ3.
    PaxDbiQ5HYJ3.
    PRIDEiQ5HYJ3.

    PTM databases

    PhosphoSiteiQ5HYJ3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ5HYJ3.
    BgeeiQ5HYJ3.
    CleanExiHS_FAM76B.
    GenevestigatoriQ5HYJ3.

    Organism-specific databases

    HPAiHPA036608.

    Interactioni

    Protein-protein interaction databases

    BioGridi126816. 8 interactions.
    IntActiQ5HYJ3. 4 interactions.
    MINTiMINT-1472951.
    STRINGi9606.ENSP00000351631.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5HYJ3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili248 – 32881Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi151 – 16010Poly-Ser
    Compositional biasi167 – 18721His-richAdd
    BLAST
    Compositional biasi171 – 18010Poly-His

    Domaini

    The polyhistidine repeat acts as a targeting signal to nuclear speckles.1 Publication

    Sequence similaritiesi

    Belongs to the FAM76 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG330390.
    HOGENOMiHOG000231316.
    HOVERGENiHBG080224.
    InParanoidiQ5HYJ3.
    OMAiNQRYPFE.
    OrthoDBiEOG7TXKGS.
    PhylomeDBiQ5HYJ3.
    TreeFamiTF313644.

    Family and domain databases

    InterProiIPR029661. FAM76B.
    [Graphical view]
    PANTHERiPTHR22875:SF9. PTHR22875:SF9. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5HYJ3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAASALYACT KCTQRYPFEE LSQGQQLCKE CRIAHPIVKC TYCRSEFQQE    50
    SKTNTICKKC AQNVKQFGTP KPCQYCNIIA AFIGTKCQRC TNSEKKYGPP 100
    QTCEQCKQQC AFDRKEEGRR KVDGKLLCWL CTLSYKRVLQ KTKEQRKSLG 150
    SSHSNSSSSS LTEKDQHHPK HHHHHHHHHH RHSSSHHKIS NLSPEEEQGL 200
    WKQSHKSSAT IQNETPKKKP KLESKPSNGD SSSINQSADS GGTDNFVLIS 250
    QLKEEVMSLK RLLQQRDQTI LEKDKKLTEL KADFQYQESN LRTKMNSMEK 300
    AHKETVEQLQ AKNRELLKQV AALSKGKKFD KSGSILTSP 339
    Length:339
    Mass (Da):38,708
    Last modified:May 18, 2010 - v3
    Checksum:i4ED7FEE05F23790C
    GO
    Isoform 2 (identifier: Q5HYJ3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         231-233: SSS → RSM
         234-339: Missing.

    Note: Ubiquitinated at position 225.Curated

    Show »
    Length:233
    Mass (Da):26,771
    Checksum:i3080A28B11A7C341
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti228 – 2281N → K in CAI46088. (PubMed:17974005)Curated
    Sequence conflicti328 – 3281K → E in CAI46088. (PubMed:17974005)Curated
    Sequence conflicti333 – 3331G → R in CAI46088. (PubMed:17974005)Curated
    Isoform 2 (identifier: Q5HYJ3-2)
    Sequence conflicti232 – 2321S → C in AAH26013. (PubMed:16554811)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei231 – 2333SSS → RSM in isoform 2. 1 PublicationVSP_022679
    Alternative sequencei234 – 339106Missing in isoform 2. 1 PublicationVSP_022680Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX647586 mRNA. Translation: CAI46088.1.
    AP001877 Genomic DNA. No translation available.
    BC026013 mRNA. Translation: AAH26013.1.
    BC028727 mRNA. Translation: AAH28727.1.
    CCDSiCCDS41700.1. [Q5HYJ3-1]
    RefSeqiNP_653265.3. NM_144664.4. [Q5HYJ3-1]
    XP_005273834.1. XM_005273777.2.
    UniGeneiHs.288304.

    Genome annotation databases

    EnsembliENST00000358780; ENSP00000351631; ENSG00000077458. [Q5HYJ3-1]
    GeneIDi143684.
    KEGGihsa:143684.
    UCSCiuc001pfn.2. human. [Q5HYJ3-1]

    Polymorphism databases

    DMDMi296439347.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX647586 mRNA. Translation: CAI46088.1 .
    AP001877 Genomic DNA. No translation available.
    BC026013 mRNA. Translation: AAH26013.1 .
    BC028727 mRNA. Translation: AAH28727.1 .
    CCDSi CCDS41700.1. [Q5HYJ3-1 ]
    RefSeqi NP_653265.3. NM_144664.4. [Q5HYJ3-1 ]
    XP_005273834.1. XM_005273777.2.
    UniGenei Hs.288304.

    3D structure databases

    ProteinModelPortali Q5HYJ3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 126816. 8 interactions.
    IntActi Q5HYJ3. 4 interactions.
    MINTi MINT-1472951.
    STRINGi 9606.ENSP00000351631.

    PTM databases

    PhosphoSitei Q5HYJ3.

    Polymorphism databases

    DMDMi 296439347.

    Proteomic databases

    MaxQBi Q5HYJ3.
    PaxDbi Q5HYJ3.
    PRIDEi Q5HYJ3.

    Protocols and materials databases

    DNASUi 143684.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000358780 ; ENSP00000351631 ; ENSG00000077458 . [Q5HYJ3-1 ]
    GeneIDi 143684.
    KEGGi hsa:143684.
    UCSCi uc001pfn.2. human. [Q5HYJ3-1 ]

    Organism-specific databases

    CTDi 143684.
    GeneCardsi GC11M095502.
    H-InvDB HIX0010043.
    HGNCi HGNC:28492. FAM76B.
    HPAi HPA036608.
    neXtProti NX_Q5HYJ3.
    PharmGKBi PA142671838.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG330390.
    HOGENOMi HOG000231316.
    HOVERGENi HBG080224.
    InParanoidi Q5HYJ3.
    OMAi NQRYPFE.
    OrthoDBi EOG7TXKGS.
    PhylomeDBi Q5HYJ3.
    TreeFami TF313644.

    Miscellaneous databases

    GeneWikii FAM76B.
    GenomeRNAii 143684.
    NextBioi 84772.
    PROi Q5HYJ3.

    Gene expression databases

    ArrayExpressi Q5HYJ3.
    Bgeei Q5HYJ3.
    CleanExi HS_FAM76B.
    Genevestigatori Q5HYJ3.

    Family and domain databases

    InterProi IPR029661. FAM76B.
    [Graphical view ]
    PANTHERi PTHR22875:SF9. PTHR22875:SF9. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Fetal skin.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Testis.
    4. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. "The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells."
      Vasilescu J., Zweitzig D.R., Denis N.J., Smith J.C., Ethier M., Haines D.S., Figeys D.
      J. Proteome Res. 6:298-305(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-225 (ISOFORM 2).
      Tissue: Lung adenocarcinoma.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    9. "Genome-wide analysis of histidine repeats reveals their role in the localization of human proteins to the nuclear speckles compartment."
      Salichs E., Ledda A., Mularoni L., Alba M.M., de la Luna S.
      PLoS Genet. 5:E1000397-E1000397(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148 AND SER-193, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFA76B_HUMAN
    AccessioniPrimary (citable) accession number: Q5HYJ3
    Secondary accession number(s): Q6PIU3, Q8TC53
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 78 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3