Q5HXU1 (DNAE2_GLUOX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	GOX2598

Encoded on

Plasmid pGOX1

Organism

Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) [Complete proteome] [HAMAP]

Taxonomic identifier

290633 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconobacter

Protein attributes

Sequence length	901 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 901	901	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103380

Sequences

	Sequence		Length	Mass (Da)
	Q5HXU1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 56F6240D81383D72	FASTA	901	100,168
	10 20 30 40 50 60 MAMYCEPRSE WWTRLPAPGG HRAWIACSSA LSRSAVLAGT PARLPVSTSA FLTHSFSVCA 70 80 90 100 110 120 EQPILLAIDT IAVQRDGCSP SCSRTRRTAR ERTSGENFFT GLLIRPSSQV NRSPLIPGRF 130 140 150 160 170 180 NPTETERTGE SAQETLTRLV QAALPRRYPA GAPPEVETQI AHELRLIGSL SYAPYFLTVN 190 200 210 220 230 240 TIVRHARSLG IVCQGRGSAA NSAVCYVLGI TSIDPVRSGL LFERFISTER QEPPDIDVDF 250 260 270 280 290 300 ESDRREEVIQ WIYRHYGRHR AALCATVQRY QPKGALREVG KVLGLPEDLT GQLSKHIASS 310 320 330 340 350 360 LADPDLCKAR AADLGLNLKD RRLSLTFHLA RLLLGFPRQL GTHPGGFVLT EDRLDELVPL 370 380 390 400 410 420 MPTAMDGRQI IVWDKDDIDV LRFMKVDVLG LGMLGCLRRG FELLHTVYQT RMDLAAIPAE 430 440 450 460 470 480 DPQTYRMVQK ADTLGTFQIE SRAQMSMLPR MKPATFYDLV IQVAIVRPGP IQGDMVHPYL 490 500 510 520 530 540 RRREKLEPVT YPSETLRGIL GKTLGVPLFQ EQAMQVAIHC AGFTPGEADQ LRRAMATFKM 550 560 570 580 590 600 TGGVSPFRDK LVNGMLANGY EQEFAEQTFA QLEGFGSYGF PESHAASFAL IAYASAWMKC 610 620 630 640 650 660 HYPDVFCAAL LNSQPMGFYA PSQIVQDAQR HGVEVRPICI NASRWDCTLE RGRNGRHAAV 670 680 690 700 710 720 RLGFRMVKGL ANGHGAALIA ARMPDYESID DVWRRADVPV AALKCLAEAD AFRVFGQMRR 730 740 750 760 770 780 AALWSIKGLA DTALPLFEAA DRGRNFPLPE VIEPEITLPM MSERASVHED YRATGLSLNG 790 800 810 820 830 840 HPVAFLREGL RKEGIVRCGD LPYLRDGRRI QLTGLVLMRQ RPGTANGTMF VTIEDETGTA 850 860 870 880 890 900 NLIVWKDVQE KYRRPLLASR LLACKGRLQK EGDVIHVVVL SLEDRTPLLQ GSEPLKARDF R

References

[1]

"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans." Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U. Nat. Biotechnol. 23:195-200(2005) [PubMed: 15665824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 621H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000004 Genomic DNA. Translation: AAW59662.1.
RefSeq	YP_190318.1. NC_006672.1.
3D structure databases
ProteinModelPortal	Q5HXU1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3248493.
GenomeReviews	Gene locus GOX2598 in contig CP000004_GR.
KEGG	gox:GOX2598.
NMPDR	fig|290633.1.peg.2527.
PATRIC	32607397. VBIGluOxy81109_0097.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734490.
OMA	RISELCE.
Enzyme and pathway databases
BioCyc	GOXY290633:GOX2598-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. Divergent sequence. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA-bd_OB_tRNA-helicase. IPR004805. PolC_alpha. [Graphical view]
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_GLUOX

Accession

Primary (citable) accession number: Q5HXU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program