Q5HWM9 (PYRE_CAMJR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	CJE0284

Organism

Campylobacter jejuni (strain RM1221) [Complete proteome] [HAMAP]

Taxonomic identifier

195099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000066219
Regions
	Region	113 – 121	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	93	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	117	1	Orotate By similarity
	Binding site	145	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HWM9 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 3F62A93148C9F668	FASTA	202	22,246
	10 20 30 40 50 60 MNLEQIYKDC GAYLEGHFLL SSGKHSQFYL QSAKVLEDPK LAAKLCDELA KIITSYKIEF 70 80 90 100 110 120 DSICSPALGG ILAGYELARA CSKRFIFTER VNKEMTLRRG FEVKKSEKFI ICEDIITTGG 130 140 150 160 170 180 SALESAKIIE SLGGIVVGFA ALANRGFCAV ENLKSPRKDN AKLPENLPLF TLGNFEFEIY 190 200 DETNCPLCKK GSKAIKPGSR GN

References

[1]

"Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species." Fouts D.E., Mongodin E.F., Mandrell R.E., Miller W.G., Rasko D.A., Ravel J., Brinkac L.M., DeBoy R.T., Parker C.T., Daugherty S.C., Dodson R.J., Durkin A.S., Madupu R., Sullivan S.A., Shetty J.U., Ayodeji M.A., Shvartsbeyn A., Schatz M.C. , Badger J.H., Fraser C.M., Nelson K.E. PLoS Biol. 3:72-85(2005) [PubMed: 15660156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RM1221.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000025 Genomic DNA. Translation: AAW34876.1.
RefSeq	YP_178306.1. NC_003912.7.
3D structure databases
ProteinModelPortal	Q5HWM9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HWM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3231046.
GenomeReviews	Gene locus CJE0284 in contig CP000025_GR.
KEGG	cjr:CJE0284.
NMPDR	fig|195099.3.peg.536.
PATRIC	20042266. VBICamJej134361_0282.
TIGR	CJE0284.
Phylogenomic databases
eggNOG	COG0461.
HOGENOM	HBG404341.
OMA	FYLQSAK.
ProtClustDB	PRK00455.
Enzyme and pathway databases
BioCyc	CJEJ195099:CJE_0284-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR023031. Orotate_PribosylTferase. IPR006273. Orotate_PRibTrfase_thermus-typ. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01367. PyrE_Therm. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_CAMJR

Accession

Primary (citable) accession number: Q5HWM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program