Reviewed, UniProtKB/Swiss-Prot Q5HWG3 (BIOD_CAMJR)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	CJE0353

Organism

Campylobacter jejuni (strain RM1221) [Complete proteome] [HAMAP]

Taxonomic identifier

195099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Dethiobiotin synthetase HAMAP MF_00336		PRO_0000302495
Regions
	Nucleotide binding	7 – 15	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HWG3-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: D20A0E57E9EE36B8	FASTA	201	22,983
	10 20 30 40 50 60 MQIYVSGIHT DVGKTHFSAA FCANFNYDYF KLIQAGTPTD SEFIAKFSPK TKIFKEGIFL 70 80 90 100 110 120 QTPASPHLGK IKEKLDYKAL DIILPKSKNL LIELAGGLFS PMDENYTMID FVNIFKHPTI 130 140 150 160 170 180 LVAKYYLGSI NHILLSIEAL KQRNINLLAL VMMGKKDILQ DDFIKNYAKI PIINLDFFDE 190 200 NSILNKDFKE QMQEILQLKI P

References

[1]

"Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species." Fouts D.E., Mongodin E.F., Mandrell R.E., Miller W.G., Rasko D.A., Ravel J., Brinkac L.M., DeBoy R.T., Parker C.T., Daugherty S.C., Dodson R.J., Durkin A.S., Madupu R., Sullivan S.A., Shetty J.U., Ayodeji M.A., Shvartsbeyn A., Schatz M.C. , Badger J.H., Fraser C.M., Nelson K.E. PLoS Biol. 3:72-85(2005) [PubMed: 15660156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000025 Genomic DNA. Translation: AAW34942.1.
RefSeq	YP_178372.1.
3D structure databases
SMR	Q5HWG3. Positions 2-154.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HWG3.
Genome annotation databases
GeneID	3231115.
GenomeReviews	Gene locus CJE0353 in contig CP000025_GR.
KEGG	cjr:CJE0353.
TIGR	CJE0353.
Phylogenomic databases
eggNOG	COG0132.
HOGENOM	HBG650065.
OMA	WKPIQSG.
Enzyme and pathway databases
BioCyc	CJEJ195099:CJE_0353-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CAMJR

Accession

Primary (citable) accession number: Q5HWG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)