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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Campylobacter jejuni (strain RM1221)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi45Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi77Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei79Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei147Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei172Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei176Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei417Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei421Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei527Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei575Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei602Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei890Substrate; via amide nitrogenUniRule annotation1
Binding sitei898Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei915Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:CJE0871
OrganismiCampylobacter jejuni (strain RM1221)
Taxonomic identifieri195099 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Tat-type signalUniRule annotationAdd BLAST30
ChainiPRO_000004598131 – 924Periplasmic nitrate reductaseUniRule annotationAdd BLAST894

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ5HV12
SMRiQ5HV12
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 914Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni209 – 216Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni552 – 553Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni814 – 823Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 2 hits
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5HV12-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRDFIKNT AIASAASVAG LSVPSSMLGA QEEDWKWDKA VCRFCGTGCG
60 70 80 90 100
IMIARKDGKI VATKGDPAAP VNRGLNCIKG YFNAKIMYGE DRLVMPLLRM
110 120 130 140 150
NEKGEFDKKG KFQQVSWQRA FDEMEKQFKK AYNELGVTGI GIFGSGQYTI
160 170 180 190 200
QEGYAALKLA KAGFRTNNID PNARHCMASA VVGFMQTFGV DEPSGCYDDI
210 220 230 240 250
ELTDTIITWG ANMAEMHPIL WSRVSDRKLS NLDKVKVVNL STFSNRTSNI
260 270 280 290 300
ADIEIIFKPN TDLAIWNYIA REIVYNHPEA MDMKFIKDHC VFATGYADIG
310 320 330 340 350
YGMRNNPNHP KFKESEKDTV EKENVITLDD EEATSLSYLG VKAGDKFEMK
360 370 380 390 400
HQGVADKNWE ISFDEFKKGL APYTLEYTAR VAKGDDNESL EDFKKKLQEL
410 420 430 440 450
ANLYIEKNRK VVSFWTMGFN QHTRGSWVNE QAYMVHFLLG KQAKPGSGAF
460 470 480 490 500
SLTGQPSACG TAREVGTFSH RLPADMVVAN PKHREISEKI WKVPAKTINP
510 520 530 540 550
KPGSPYLNIM RDLEDGKIKF AWVQVNNPWQ NTANANHWIA AAREMDNFIV
560 570 580 590 600
VSDCYPGISA KVADLILPSA MIYEKWGAYG NAERRTQHWK QQVLPVGAAM
610 620 630 640 650
SDTWQILEFA KRFKLKEVWK EQKVDNKLTL PSVLEEAKAM GYSEDDTLFD
660 670 680 690 700
VLFANKEAKS FNPNDAIAKG FDNTDVKGDE RKIQGSDGKE FTGYGFFVQK
710 720 730 740 750
YLWEEYRKFG LGHGHDLADF DTYHKVRGLR WPVVNGKETQ WRFNTKFDYY
760 770 780 790 800
AKKAAPNSDF AFYGDFNKML TNGDLIAPKD EKEHSIKNKA KIFFRPFMKA
810 820 830 840 850
PERPSKEYPF WLATGRVLEH WHSGTMTMRV PELYRAVPEA LCYMSEKDGE
860 870 880 890 900
KLGLNQGDLV WVESRRGKVK ARVDMRGRNK PPVGLVYVPW FDENVYINKV
910 920
TLDATCPLSK QTDFKKCAVK IYKA
Length:924
Mass (Da):104,944
Last modified:February 15, 2005 - v1
Checksum:i9DA5E87E0263BC9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000025 Genomic DNA Translation: AAW35208.1
RefSeqiWP_002852404.1, NC_003912.7

Genome annotation databases

EnsemblBacteriaiAAW35208; AAW35208; CJE0871
KEGGicjr:CJE0871

Similar proteinsi

Entry informationi

Entry nameiNAPA_CAMJR
AccessioniPrimary (citable) accession number: Q5HV12
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 15, 2005
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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