Reviewed, UniProtKB/Swiss-Prot Q5HUR4 (ISPH_CAMJR)

Names and origin

Protein names

Recommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
    EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	CJE0973

Organism

Campylobacter jejuni (strain RM1221) [Complete proteome] [HAMAP]

Taxonomic identifier

195099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity.
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity.
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the ispH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_0000128796

Sequences

	Sequence		Length	Mass (Da)
	Q5HUR4-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: BD428735DC28C73A	FASTA	277	31,523
	10 20 30 40 50 60 MIIELAKNYG FCFGVKRAIK KAEQIKDAAT IGPLIHNNEE ISRLQKNFNV KTLENIQALS 70 80 90 100 110 120 NEKKAIIRTH GITKQDLEEL RKKDIEIFDA TCPFVTKPQQ ICEQMSKEGY EVVIFGDENH 130 140 150 160 170 180 PEVKGVKSYV STKAYVVLDK KELQNIKLPN KIAVVSQTTK KPEHFMEIVN FLILKTKEVR 190 200 210 220 230 240 VFNTICDATF KNQDAIKELS LKSDVMVVVG GKNSANTKQL FLIAKTNCED SYLIETEEEL 250 260 270 KKEWFLDKKH CGISAGASTP DWIIQKVIAK IENFKIN

References

[1]

"Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species." Fouts D.E., Mongodin E.F., Mandrell R.E., Miller W.G., Rasko D.A., Ravel J., Brinkac L.M., DeBoy R.T., Parker C.T., Daugherty S.C., Dodson R.J., Durkin A.S., Madupu R., Sullivan S.A., Shetty J.U., Ayodeji M.A., Shvartsbeyn A., Schatz M.C. , Badger J.H., Fraser C.M., Nelson K.E. PLoS Biol. 3:72-85(2005) [PubMed: 15660156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000025 Genomic DNA. Translation: AAW35306.1.
RefSeq	YP_178971.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HUR4.
Genome annotation databases
GeneID	3231484.
GenomeReviews	Gene locus CJE0973 in contig CP000025_GR.
KEGG	cjr:CJE0973.
TIGR	CJE0973.
Phylogenomic databases
HOGENOM	Q5HUR4.
OMA	KNDICYA.
Enzyme and pathway databases
BioCyc	CJEJ195099:CJE_0973-MON.
Family and domain databases
HAMAP	MF_00191. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. ispH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_CAMJR

Accession

Primary (citable) accession number: Q5HUR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	February 15, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)